From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin
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From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin. / Huck, Volker; Gorzelanny, Christian; Thomas, Kai; Getova, Valentina; Niemeyer, Verena; Zens, Katharina; Unnerstall, Tim R; Feger, Julia S; Fallah, Mohammad A; Metze, Dieter; Ständer, Sonja; Luger, Thomas A; Koenig, Karsten; Mess, Christian; Schneider, Stefan W.
In: SCI REP-UK, Vol. 6, 23.03.2016, p. 22789.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin
AU - Huck, Volker
AU - Gorzelanny, Christian
AU - Thomas, Kai
AU - Getova, Valentina
AU - Niemeyer, Verena
AU - Zens, Katharina
AU - Unnerstall, Tim R
AU - Feger, Julia S
AU - Fallah, Mohammad A
AU - Metze, Dieter
AU - Ständer, Sonja
AU - Luger, Thomas A
AU - Koenig, Karsten
AU - Mess, Christian
AU - Schneider, Stefan W
PY - 2016/3/23
Y1 - 2016/3/23
N2 - The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients' bedsides. These 'optical biopsies' generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.
AB - The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients' bedsides. These 'optical biopsies' generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.
KW - Algorithms
KW - Biopsy
KW - Cell Nucleus
KW - Cells, Cultured
KW - Dermatitis, Atopic
KW - Humans
KW - Keratinocytes
KW - Microscopy, Fluorescence, Multiphoton
KW - Mitochondria
KW - Skin
KW - Tomography, Optical
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1038/srep22789
DO - 10.1038/srep22789
M3 - SCORING: Journal article
C2 - 27004454
VL - 6
SP - 22789
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
ER -