PortalPublikationenFrom morphology to biochemical state - intravital multiphoto...

From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin

Standard

From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin. / Huck, Volker; Gorzelanny, Christian; Thomas, Kai; Getova, Valentina; Niemeyer, Verena; Zens, Katharina; Unnerstall, Tim R; Feger, Julia S; Fallah, Mohammad A; Metze, Dieter; Ständer, Sonja; Luger, Thomas A; Koenig, Karsten; Mess, Christian; Schneider, Stefan W.

in: SCI REP-UK, Jahrgang 6, 23.03.2016, S. 22789.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Huck, V, Gorzelanny, C, Thomas, K, Getova, V, Niemeyer, V, Zens, K, Unnerstall, TR, Feger, JS, Fallah, MA, Metze, D, Ständer, S, Luger, TA, Koenig, K, Mess, C & Schneider, SW 2016, 'From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin', SCI REP-UK, Jg. 6, S. 22789. https://doi.org/10.1038/srep22789

APA

Huck, V., Gorzelanny, C., Thomas, K., Getova, V., Niemeyer, V., Zens, K., Unnerstall, T. R., Feger, J. S., Fallah, M. A., Metze, D., Ständer, S., Luger, T. A., Koenig, K., Mess, C., & Schneider, S. W. (2016). From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin. SCI REP-UK, 6, 22789. https://doi.org/10.1038/srep22789

Vancouver

Huck V, Gorzelanny C, Thomas K, Getova V, Niemeyer V, Zens K et al. From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin. SCI REP-UK. 2016 Mär 23;6:22789. https://doi.org/10.1038/srep22789

Bibtex

@article{4911d5df38014e1ca27705029a5c2881,
title = "From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin",
abstract = "The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients' bedsides. These 'optical biopsies' generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.",
keywords = "Algorithms, Biopsy, Cell Nucleus, Cells, Cultured, Dermatitis, Atopic, Humans, Keratinocytes, Microscopy, Fluorescence, Multiphoton, Mitochondria, Skin, Tomography, Optical, Journal Article, Research Support, Non-U.S. Gov't",
author = "Volker Huck and Christian Gorzelanny and Kai Thomas and Valentina Getova and Verena Niemeyer and Katharina Zens and Unnerstall, {Tim R} and Feger, {Julia S} and Fallah, {Mohammad A} and Dieter Metze and Sonja St{\"a}nder and Luger, {Thomas A} and Karsten Koenig and Christian Mess and Schneider, {Stefan W}",
year = "2016",
month = mar,
day = "23",
doi = "10.1038/srep22789",
language = "English",
volume = "6",
pages = "22789",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin

AU - Huck, Volker

AU - Gorzelanny, Christian

AU - Thomas, Kai

AU - Getova, Valentina

AU - Niemeyer, Verena

AU - Zens, Katharina

AU - Unnerstall, Tim R

AU - Feger, Julia S

AU - Fallah, Mohammad A

AU - Metze, Dieter

AU - Ständer, Sonja

AU - Luger, Thomas A

AU - Koenig, Karsten

AU - Mess, Christian

AU - Schneider, Stefan W

PY - 2016/3/23

Y1 - 2016/3/23

N2 - The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients' bedsides. These 'optical biopsies' generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.

AB - The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients' bedsides. These 'optical biopsies' generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.

KW - Algorithms

KW - Biopsy

KW - Cell Nucleus

KW - Cells, Cultured

KW - Dermatitis, Atopic

KW - Humans

KW - Keratinocytes

KW - Microscopy, Fluorescence, Multiphoton

KW - Mitochondria

KW - Skin

KW - Tomography, Optical

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/srep22789

DO - 10.1038/srep22789

M3 - SCORING: Journal article

C2 - 27004454

VL - 6

SP - 22789

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

ER -