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Freeze-frame imaging of synaptic activity using SynTagMA

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Freeze-frame imaging of synaptic activity using SynTagMA. / Perez-Alvarez, Alberto; Fearey, Brenna C; O'Toole, Ryan J; Yang, Wei; Arganda-Carreras, Ignacio; Lamothe-Molina, Paul J; Moeyaert, Benjamien; Mohr, Manuel A; Panzera, Lauren C; Schulze, Christian; Schreiter, Eric R; Wiegert, J Simon; Gee, Christine E; Hoppa, Michael B; Oertner, Thomas G.

In: NAT COMMUN, Vol. 11, No. 1, 18.05.2020, p. 2464.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Perez-Alvarez, A, Fearey, BC, O'Toole, RJ, Yang, W, Arganda-Carreras, I, Lamothe-Molina, PJ, Moeyaert, B, Mohr, MA, Panzera, LC, Schulze, C, Schreiter, ER, Wiegert, JS, Gee, CE, Hoppa, MB & Oertner, TG 2020, 'Freeze-frame imaging of synaptic activity using SynTagMA', NAT COMMUN, vol. 11, no. 1, pp. 2464. https://doi.org/10.1038/s41467-020-16315-4

APA

Perez-Alvarez, A., Fearey, B. C., O'Toole, R. J., Yang, W., Arganda-Carreras, I., Lamothe-Molina, P. J., Moeyaert, B., Mohr, M. A., Panzera, L. C., Schulze, C., Schreiter, E. R., Wiegert, J. S., Gee, C. E., Hoppa, M. B., & Oertner, T. G. (2020). Freeze-frame imaging of synaptic activity using SynTagMA. NAT COMMUN, 11(1), 2464. https://doi.org/10.1038/s41467-020-16315-4

Vancouver

Perez-Alvarez A, Fearey BC, O'Toole RJ, Yang W, Arganda-Carreras I, Lamothe-Molina PJ et al. Freeze-frame imaging of synaptic activity using SynTagMA. NAT COMMUN. 2020 May 18;11(1):2464. https://doi.org/10.1038/s41467-020-16315-4

Bibtex

@article{d8ce04b18acb41b0b3b89730c54fc8bc,
title = "Freeze-frame imaging of synaptic activity using SynTagMA",
abstract = "Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a user-defined time window. Upon 395-405 nm illumination, this genetically encoded marker of activity converts from green to red fluorescence if, and only if, it is bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we show how to identify and track the fluorescence of thousands of individual synapses in an automated fashion. Together, these tools provide an efficient method for repeatedly mapping active neurons and synapses in cell culture, slice preparations, and in vivo during behavior.",
keywords = "Action Potentials, Animals, Axons/metabolism, Biomarkers/metabolism, Cells, Cultured, Female, Fluorescence, Hippocampus/cytology, Imaging, Three-Dimensional, Light, Male, Mice, Inbred C57BL, Neurons/metabolism, Presynaptic Terminals/metabolism, Rats, Sprague-Dawley, Rats, Wistar, Synapses/physiology, Synaptophysin/metabolism, Time Factors",
author = "Alberto Perez-Alvarez and Fearey, {Brenna C} and O'Toole, {Ryan J} and Wei Yang and Ignacio Arganda-Carreras and Lamothe-Molina, {Paul J} and Benjamien Moeyaert and Mohr, {Manuel A} and Panzera, {Lauren C} and Christian Schulze and Schreiter, {Eric R} and Wiegert, {J Simon} and Gee, {Christine E} and Hoppa, {Michael B} and Oertner, {Thomas G}",
year = "2020",
month = may,
day = "18",
doi = "10.1038/s41467-020-16315-4",
language = "English",
volume = "11",
pages = "2464",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Freeze-frame imaging of synaptic activity using SynTagMA

AU - Perez-Alvarez, Alberto

AU - Fearey, Brenna C

AU - O'Toole, Ryan J

AU - Yang, Wei

AU - Arganda-Carreras, Ignacio

AU - Lamothe-Molina, Paul J

AU - Moeyaert, Benjamien

AU - Mohr, Manuel A

AU - Panzera, Lauren C

AU - Schulze, Christian

AU - Schreiter, Eric R

AU - Wiegert, J Simon

AU - Gee, Christine E

AU - Hoppa, Michael B

AU - Oertner, Thomas G

PY - 2020/5/18

Y1 - 2020/5/18

N2 - Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a user-defined time window. Upon 395-405 nm illumination, this genetically encoded marker of activity converts from green to red fluorescence if, and only if, it is bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we show how to identify and track the fluorescence of thousands of individual synapses in an automated fashion. Together, these tools provide an efficient method for repeatedly mapping active neurons and synapses in cell culture, slice preparations, and in vivo during behavior.

AB - Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a user-defined time window. Upon 395-405 nm illumination, this genetically encoded marker of activity converts from green to red fluorescence if, and only if, it is bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we show how to identify and track the fluorescence of thousands of individual synapses in an automated fashion. Together, these tools provide an efficient method for repeatedly mapping active neurons and synapses in cell culture, slice preparations, and in vivo during behavior.

KW - Action Potentials

KW - Animals

KW - Axons/metabolism

KW - Biomarkers/metabolism

KW - Cells, Cultured

KW - Female

KW - Fluorescence

KW - Hippocampus/cytology

KW - Imaging, Three-Dimensional

KW - Light

KW - Male

KW - Mice, Inbred C57BL

KW - Neurons/metabolism

KW - Presynaptic Terminals/metabolism

KW - Rats, Sprague-Dawley

KW - Rats, Wistar

KW - Synapses/physiology

KW - Synaptophysin/metabolism

KW - Time Factors

U2 - 10.1038/s41467-020-16315-4

DO - 10.1038/s41467-020-16315-4

M3 - SCORING: Journal article

C2 - 32424147

VL - 11

SP - 2464

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -