Freeze-frame imaging of synaptic activity using SynTagMA
Standard
Freeze-frame imaging of synaptic activity using SynTagMA. / Perez-Alvarez, Alberto; Fearey, Brenna C; O'Toole, Ryan J; Yang, Wei; Arganda-Carreras, Ignacio; Lamothe-Molina, Paul J; Moeyaert, Benjamien; Mohr, Manuel A; Panzera, Lauren C; Schulze, Christian; Schreiter, Eric R; Wiegert, J Simon; Gee, Christine E; Hoppa, Michael B; Oertner, Thomas G.
in: NAT COMMUN, Jahrgang 11, Nr. 1, 18.05.2020, S. 2464.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Freeze-frame imaging of synaptic activity using SynTagMA
AU - Perez-Alvarez, Alberto
AU - Fearey, Brenna C
AU - O'Toole, Ryan J
AU - Yang, Wei
AU - Arganda-Carreras, Ignacio
AU - Lamothe-Molina, Paul J
AU - Moeyaert, Benjamien
AU - Mohr, Manuel A
AU - Panzera, Lauren C
AU - Schulze, Christian
AU - Schreiter, Eric R
AU - Wiegert, J Simon
AU - Gee, Christine E
AU - Hoppa, Michael B
AU - Oertner, Thomas G
PY - 2020/5/18
Y1 - 2020/5/18
N2 - Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a user-defined time window. Upon 395-405 nm illumination, this genetically encoded marker of activity converts from green to red fluorescence if, and only if, it is bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we show how to identify and track the fluorescence of thousands of individual synapses in an automated fashion. Together, these tools provide an efficient method for repeatedly mapping active neurons and synapses in cell culture, slice preparations, and in vivo during behavior.
AB - Information within the brain travels from neuron to neuron across billions of synapses. At any given moment, only a small subset of neurons and synapses are active, but finding the active synapses in brain tissue has been a technical challenge. Here we introduce SynTagMA to tag active synapses in a user-defined time window. Upon 395-405 nm illumination, this genetically encoded marker of activity converts from green to red fluorescence if, and only if, it is bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we show how to identify and track the fluorescence of thousands of individual synapses in an automated fashion. Together, these tools provide an efficient method for repeatedly mapping active neurons and synapses in cell culture, slice preparations, and in vivo during behavior.
KW - Action Potentials
KW - Animals
KW - Axons/metabolism
KW - Biomarkers/metabolism
KW - Cells, Cultured
KW - Female
KW - Fluorescence
KW - Hippocampus/cytology
KW - Imaging, Three-Dimensional
KW - Light
KW - Male
KW - Mice, Inbred C57BL
KW - Neurons/metabolism
KW - Presynaptic Terminals/metabolism
KW - Rats, Sprague-Dawley
KW - Rats, Wistar
KW - Synapses/physiology
KW - Synaptophysin/metabolism
KW - Time Factors
U2 - 10.1038/s41467-020-16315-4
DO - 10.1038/s41467-020-16315-4
M3 - SCORING: Journal article
C2 - 32424147
VL - 11
SP - 2464
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
IS - 1
ER -