Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins
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Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins. / Paulin-Levasseur, M; Scherbarth, A; Giese, G; Röser, K; Bohn, W; Traub, P.
In: J CELL SCI, Vol. 92 ( Pt 3), 03.1989, p. 361-70.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins
AU - Paulin-Levasseur, M
AU - Scherbarth, A
AU - Giese, G
AU - Röser, K
AU - Bohn, W
AU - Traub, P
PY - 1989/3
Y1 - 1989/3
N2 - Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC-11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma-derived cells SW-13 and the clone C6-M-D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA-treated MPC-11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW-13 and C6-M-D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells.
AB - Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC-11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma-derived cells SW-13 and the clone C6-M-D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA-treated MPC-11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW-13 and C6-M-D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells.
KW - Adrenal Cortex Neoplasms
KW - Animals
KW - Cell Nucleus
KW - Cytoplasm
KW - Fluorescent Antibody Technique
KW - Humans
KW - Immunoblotting
KW - Intermediate Filament Proteins
KW - Lamin Type B
KW - Lamins
KW - Mice
KW - Nuclear Proteins
KW - Plasmacytoma
KW - Rats
KW - Tetradecanoylphorbol Acetate
KW - Tumor Cells, Cultured
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 2687302
VL - 92 ( Pt 3)
SP - 361
EP - 370
JO - J CELL SCI
JF - J CELL SCI
SN - 0021-9533
ER -