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Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins

Standard

Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins. / Paulin-Levasseur, M; Scherbarth, A; Giese, G; Röser, K; Bohn, W; Traub, P.

in: J CELL SCI, Jahrgang 92 ( Pt 3), 03.1989, S. 361-70.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Paulin-Levasseur, M, Scherbarth, A, Giese, G, Röser, K, Bohn, W & Traub, P 1989, 'Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins', J CELL SCI, Jg. 92 ( Pt 3), S. 361-70.

APA

Paulin-Levasseur, M., Scherbarth, A., Giese, G., Röser, K., Bohn, W., & Traub, P. (1989). Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins. J CELL SCI, 92 ( Pt 3), 361-70.

Vancouver

Paulin-Levasseur M, Scherbarth A, Giese G, Röser K, Bohn W, Traub P. Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins. J CELL SCI. 1989 Mär;92 ( Pt 3):361-70.

Bibtex

@article{3f14577c32984838b1ea45e324265b16,
title = "Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins",
abstract = "Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC-11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma-derived cells SW-13 and the clone C6-M-D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA-treated MPC-11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW-13 and C6-M-D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells.",
keywords = "Adrenal Cortex Neoplasms, Animals, Cell Nucleus, Cytoplasm, Fluorescent Antibody Technique, Humans, Immunoblotting, Intermediate Filament Proteins, Lamin Type B, Lamins, Mice, Nuclear Proteins, Plasmacytoma, Rats, Tetradecanoylphorbol Acetate, Tumor Cells, Cultured, Journal Article, Research Support, Non-U.S. Gov't",
author = "M Paulin-Levasseur and A Scherbarth and G Giese and K R{\"o}ser and W Bohn and P Traub",
year = "1989",
month = mar,
language = "English",
volume = "92 ( Pt 3)",
pages = "361--70",
journal = "J CELL SCI",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",

}

RIS

TY - JOUR

T1 - Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins

AU - Paulin-Levasseur, M

AU - Scherbarth, A

AU - Giese, G

AU - Röser, K

AU - Bohn, W

AU - Traub, P

PY - 1989/3

Y1 - 1989/3

N2 - Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC-11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma-derived cells SW-13 and the clone C6-M-D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA-treated MPC-11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW-13 and C6-M-D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells.

AB - Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC-11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma-derived cells SW-13 and the clone C6-M-D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA-treated MPC-11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW-13 and C6-M-D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells.

KW - Adrenal Cortex Neoplasms

KW - Animals

KW - Cell Nucleus

KW - Cytoplasm

KW - Fluorescent Antibody Technique

KW - Humans

KW - Immunoblotting

KW - Intermediate Filament Proteins

KW - Lamin Type B

KW - Lamins

KW - Mice

KW - Nuclear Proteins

KW - Plasmacytoma

KW - Rats

KW - Tetradecanoylphorbol Acetate

KW - Tumor Cells, Cultured

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 2687302

VL - 92 ( Pt 3)

SP - 361

EP - 370

JO - J CELL SCI

JF - J CELL SCI

SN - 0021-9533

ER -