Experimental evaluation of aniline and methyl blue for intraocular surgery
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Experimental evaluation of aniline and methyl blue for intraocular surgery. / Haritoglou, Christos; Priglinger, Siegfried; Liegl, Raffael; May, Christian A; Eibl, Kirsten; Thaler, Sebastian; Kampik, Anselm; Schuettauf, Frank.
In: RETINA-J RET VIT DIS, Vol. 29, No. 9, 10.2009, p. 1266-73.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Experimental evaluation of aniline and methyl blue for intraocular surgery
AU - Haritoglou, Christos
AU - Priglinger, Siegfried
AU - Liegl, Raffael
AU - May, Christian A
AU - Eibl, Kirsten
AU - Thaler, Sebastian
AU - Kampik, Anselm
AU - Schuettauf, Frank
PY - 2009/10
Y1 - 2009/10
N2 - PURPOSE: The purpose of this study was to investigate the biocompatibility of aniline and methyl blue in a well-established cell culture model and assess the staining properties of these dyes at the level of the internal limiting membrane (ILM) in human donor eyes.METHODS: Dye-related toxicity was evaluated by a colorimetric test (MTT) measuring the inhibition of retinal pigment epithelium (ARPE-19 and primary human retinal pigment epithelium) cell proliferation. Cell viability was also quantified based on a two-color fluorescence assay (Life-Dead Assay). Aniline blue and methyl blue at a concentration of 0.2% was applied over the macula during vitrectomy in human donor eyes to evaluate the staining properties at the level of the ILM.RESULTS: Both dyes and dye concentrations of 0.1% and 0.2% showed no toxic effect on ARPE-19 and primary human retinal pigment epithelium cell proliferation for exposure times of 1 and 10 minutes, respectively. Cell viability was also not affected at all. Both dyes provided a good contrast at the level of the ILM and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted.CONCLUSION: Our results indicate that aniline blue and methyl blue might be applicable for intraocular surgery, providing a very good biocompatibility and required selective staining characteristics at the level of the ILM.
AB - PURPOSE: The purpose of this study was to investigate the biocompatibility of aniline and methyl blue in a well-established cell culture model and assess the staining properties of these dyes at the level of the internal limiting membrane (ILM) in human donor eyes.METHODS: Dye-related toxicity was evaluated by a colorimetric test (MTT) measuring the inhibition of retinal pigment epithelium (ARPE-19 and primary human retinal pigment epithelium) cell proliferation. Cell viability was also quantified based on a two-color fluorescence assay (Life-Dead Assay). Aniline blue and methyl blue at a concentration of 0.2% was applied over the macula during vitrectomy in human donor eyes to evaluate the staining properties at the level of the ILM.RESULTS: Both dyes and dye concentrations of 0.1% and 0.2% showed no toxic effect on ARPE-19 and primary human retinal pigment epithelium cell proliferation for exposure times of 1 and 10 minutes, respectively. Cell viability was also not affected at all. Both dyes provided a good contrast at the level of the ILM and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted.CONCLUSION: Our results indicate that aniline blue and methyl blue might be applicable for intraocular surgery, providing a very good biocompatibility and required selective staining characteristics at the level of the ILM.
KW - Adult
KW - Aged
KW - Aniline Compounds/toxicity
KW - Basement Membrane
KW - Benzenesulfonates/toxicity
KW - Cell Line
KW - Cell Proliferation/drug effects
KW - Cell Survival/drug effects
KW - Coloring Agents/toxicity
KW - Fluorescent Dyes/toxicity
KW - Humans
KW - Middle Aged
KW - Retinal Diseases/surgery
KW - Retinal Pigment Epithelium/drug effects
U2 - 10.1097/IAE.0b013e3181b8626f
DO - 10.1097/IAE.0b013e3181b8626f
M3 - SCORING: Journal article
C2 - 19934821
VL - 29
SP - 1266
EP - 1273
JO - RETINA-J RET VIT DIS
JF - RETINA-J RET VIT DIS
SN - 0275-004X
IS - 9
ER -