Experimental evaluation of aniline and methyl blue for intraocular surgery

TY - JOUR

T1 - Experimental evaluation of aniline and methyl blue for intraocular surgery

AU - Haritoglou, Christos

AU - Priglinger, Siegfried

AU - Liegl, Raffael

AU - May, Christian A

AU - Eibl, Kirsten

AU - Thaler, Sebastian

AU - Kampik, Anselm

AU - Schuettauf, Frank

PY - 2009/10

Y1 - 2009/10

N2 - PURPOSE: The purpose of this study was to investigate the biocompatibility of aniline and methyl blue in a well-established cell culture model and assess the staining properties of these dyes at the level of the internal limiting membrane (ILM) in human donor eyes.METHODS: Dye-related toxicity was evaluated by a colorimetric test (MTT) measuring the inhibition of retinal pigment epithelium (ARPE-19 and primary human retinal pigment epithelium) cell proliferation. Cell viability was also quantified based on a two-color fluorescence assay (Life-Dead Assay). Aniline blue and methyl blue at a concentration of 0.2% was applied over the macula during vitrectomy in human donor eyes to evaluate the staining properties at the level of the ILM.RESULTS: Both dyes and dye concentrations of 0.1% and 0.2% showed no toxic effect on ARPE-19 and primary human retinal pigment epithelium cell proliferation for exposure times of 1 and 10 minutes, respectively. Cell viability was also not affected at all. Both dyes provided a good contrast at the level of the ILM and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted.CONCLUSION: Our results indicate that aniline blue and methyl blue might be applicable for intraocular surgery, providing a very good biocompatibility and required selective staining characteristics at the level of the ILM.

AB - PURPOSE: The purpose of this study was to investigate the biocompatibility of aniline and methyl blue in a well-established cell culture model and assess the staining properties of these dyes at the level of the internal limiting membrane (ILM) in human donor eyes.METHODS: Dye-related toxicity was evaluated by a colorimetric test (MTT) measuring the inhibition of retinal pigment epithelium (ARPE-19 and primary human retinal pigment epithelium) cell proliferation. Cell viability was also quantified based on a two-color fluorescence assay (Life-Dead Assay). Aniline blue and methyl blue at a concentration of 0.2% was applied over the macula during vitrectomy in human donor eyes to evaluate the staining properties at the level of the ILM.RESULTS: Both dyes and dye concentrations of 0.1% and 0.2% showed no toxic effect on ARPE-19 and primary human retinal pigment epithelium cell proliferation for exposure times of 1 and 10 minutes, respectively. Cell viability was also not affected at all. Both dyes provided a good contrast at the level of the ILM and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted.CONCLUSION: Our results indicate that aniline blue and methyl blue might be applicable for intraocular surgery, providing a very good biocompatibility and required selective staining characteristics at the level of the ILM.

KW - Adult

KW - Aged

KW - Aniline Compounds/toxicity

KW - Basement Membrane

KW - Benzenesulfonates/toxicity

KW - Cell Line

KW - Cell Proliferation/drug effects

KW - Cell Survival/drug effects

KW - Coloring Agents/toxicity

KW - Fluorescent Dyes/toxicity

KW - Humans

KW - Middle Aged

KW - Retinal Diseases/surgery

KW - Retinal Pigment Epithelium/drug effects

U2 - 10.1097/IAE.0b013e3181b8626f

DO - 10.1097/IAE.0b013e3181b8626f

M3 - SCORING: Journal article

C2 - 19934821

VL - 29

SP - 1266

EP - 1273

JO - RETINA-J RET VIT DIS

JF - RETINA-J RET VIT DIS

SN - 0275-004X

IS - 9

ER -