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Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics

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Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics. / Terzoli, Sara; Marzano, Paolo; Cazzetta, Valentina; Piazza, Rocco; Sandrock, Inga; Ravens, Sarina; Tan, Likai; Prinz, Immo; Balin, Simone; Calvi, Michela; Carletti, Anna; Cancellara, Assunta; Coianiz, Nicolò; Franzese, Sara; Frigo, Alessandro; Voza, Antonio; Calcaterra, Francesca; Di Vito, Clara; Della Bella, Silvia; Mikulak, Joanna; Mavilio, Domenico.

In: NPJ VACCINES, Vol. 9, No. 1, 20.03.2024, p. 63.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Terzoli, S, Marzano, P, Cazzetta, V, Piazza, R, Sandrock, I, Ravens, S, Tan, L, Prinz, I, Balin, S, Calvi, M, Carletti, A, Cancellara, A, Coianiz, N, Franzese, S, Frigo, A, Voza, A, Calcaterra, F, Di Vito, C, Della Bella, S, Mikulak, J & Mavilio, D 2024, 'Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics', NPJ VACCINES, vol. 9, no. 1, pp. 63. https://doi.org/10.1038/s41541-024-00853-9

APA

Terzoli, S., Marzano, P., Cazzetta, V., Piazza, R., Sandrock, I., Ravens, S., Tan, L., Prinz, I., Balin, S., Calvi, M., Carletti, A., Cancellara, A., Coianiz, N., Franzese, S., Frigo, A., Voza, A., Calcaterra, F., Di Vito, C., Della Bella, S., ... Mavilio, D. (2024). Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics. NPJ VACCINES, 9(1), 63. https://doi.org/10.1038/s41541-024-00853-9

Vancouver

Terzoli S, Marzano P, Cazzetta V, Piazza R, Sandrock I, Ravens S et al. Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics. NPJ VACCINES. 2024 Mar 20;9(1):63. https://doi.org/10.1038/s41541-024-00853-9

Bibtex

@article{be17219ed135459ba23e5dea2af98c23,
title = "Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics",
abstract = "γδ T cells provide rapid cellular immunity against pathogens. Here, we conducted matched single-cell RNA-sequencing and γδ-TCR-sequencing to delineate the molecular changes in γδ T cells during a longitudinal study following mRNA SARS-CoV-2 vaccination. While the first dose of vaccine primes Vδ2 T cells, it is the second administration that significantly boosts their immune response. Specifically, the second vaccination uncovers memory features of Vδ2 T cells, shaped by the induction of AP-1 family transcription factors and characterized by a convergent central memory signature, clonal expansion, and an enhanced effector potential. This temporally distinct effector response of Vδ2 T cells was also confirmed in vitro upon stimulation with SARS-CoV-2 spike-peptides. Indeed, the second challenge triggers a significantly higher production of IFNγ by Vδ2 T cells. Collectively, our findings suggest that mRNA SARS-CoV-2 vaccination might benefit from the establishment of long-lasting central memory Vδ2 T cells to confer protection against SARS-CoV-2 infection.",
author = "Sara Terzoli and Paolo Marzano and Valentina Cazzetta and Rocco Piazza and Inga Sandrock and Sarina Ravens and Likai Tan and Immo Prinz and Simone Balin and Michela Calvi and Anna Carletti and Assunta Cancellara and Nicol{\`o} Coianiz and Sara Franzese and Alessandro Frigo and Antonio Voza and Francesca Calcaterra and {Di Vito}, Clara and {Della Bella}, Silvia and Joanna Mikulak and Domenico Mavilio",
note = "{\textcopyright} 2024. The Author(s).",
year = "2024",
month = mar,
day = "20",
doi = "10.1038/s41541-024-00853-9",
language = "English",
volume = "9",
pages = "63",
journal = "NPJ VACCINES",
issn = "2059-0105",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics

AU - Terzoli, Sara

AU - Marzano, Paolo

AU - Cazzetta, Valentina

AU - Piazza, Rocco

AU - Sandrock, Inga

AU - Ravens, Sarina

AU - Tan, Likai

AU - Prinz, Immo

AU - Balin, Simone

AU - Calvi, Michela

AU - Carletti, Anna

AU - Cancellara, Assunta

AU - Coianiz, Nicolò

AU - Franzese, Sara

AU - Frigo, Alessandro

AU - Voza, Antonio

AU - Calcaterra, Francesca

AU - Di Vito, Clara

AU - Della Bella, Silvia

AU - Mikulak, Joanna

AU - Mavilio, Domenico

N1 - © 2024. The Author(s).

PY - 2024/3/20

Y1 - 2024/3/20

N2 - γδ T cells provide rapid cellular immunity against pathogens. Here, we conducted matched single-cell RNA-sequencing and γδ-TCR-sequencing to delineate the molecular changes in γδ T cells during a longitudinal study following mRNA SARS-CoV-2 vaccination. While the first dose of vaccine primes Vδ2 T cells, it is the second administration that significantly boosts their immune response. Specifically, the second vaccination uncovers memory features of Vδ2 T cells, shaped by the induction of AP-1 family transcription factors and characterized by a convergent central memory signature, clonal expansion, and an enhanced effector potential. This temporally distinct effector response of Vδ2 T cells was also confirmed in vitro upon stimulation with SARS-CoV-2 spike-peptides. Indeed, the second challenge triggers a significantly higher production of IFNγ by Vδ2 T cells. Collectively, our findings suggest that mRNA SARS-CoV-2 vaccination might benefit from the establishment of long-lasting central memory Vδ2 T cells to confer protection against SARS-CoV-2 infection.

AB - γδ T cells provide rapid cellular immunity against pathogens. Here, we conducted matched single-cell RNA-sequencing and γδ-TCR-sequencing to delineate the molecular changes in γδ T cells during a longitudinal study following mRNA SARS-CoV-2 vaccination. While the first dose of vaccine primes Vδ2 T cells, it is the second administration that significantly boosts their immune response. Specifically, the second vaccination uncovers memory features of Vδ2 T cells, shaped by the induction of AP-1 family transcription factors and characterized by a convergent central memory signature, clonal expansion, and an enhanced effector potential. This temporally distinct effector response of Vδ2 T cells was also confirmed in vitro upon stimulation with SARS-CoV-2 spike-peptides. Indeed, the second challenge triggers a significantly higher production of IFNγ by Vδ2 T cells. Collectively, our findings suggest that mRNA SARS-CoV-2 vaccination might benefit from the establishment of long-lasting central memory Vδ2 T cells to confer protection against SARS-CoV-2 infection.

U2 - 10.1038/s41541-024-00853-9

DO - 10.1038/s41541-024-00853-9

M3 - SCORING: Journal article

C2 - 38509155

VL - 9

SP - 63

JO - NPJ VACCINES

JF - NPJ VACCINES

SN - 2059-0105

IS - 1

ER -