Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics
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Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics. / Terzoli, Sara; Marzano, Paolo; Cazzetta, Valentina; Piazza, Rocco; Sandrock, Inga; Ravens, Sarina; Tan, Likai; Prinz, Immo; Balin, Simone; Calvi, Michela; Carletti, Anna; Cancellara, Assunta; Coianiz, Nicolò; Franzese, Sara; Frigo, Alessandro; Voza, Antonio; Calcaterra, Francesca; Di Vito, Clara; Della Bella, Silvia; Mikulak, Joanna; Mavilio, Domenico.
in: NPJ VACCINES, Jahrgang 9, Nr. 1, 20.03.2024, S. 63.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Expansion of memory Vδ2 T cells following SARS-CoV-2 vaccination revealed by temporal single-cell transcriptomics
AU - Terzoli, Sara
AU - Marzano, Paolo
AU - Cazzetta, Valentina
AU - Piazza, Rocco
AU - Sandrock, Inga
AU - Ravens, Sarina
AU - Tan, Likai
AU - Prinz, Immo
AU - Balin, Simone
AU - Calvi, Michela
AU - Carletti, Anna
AU - Cancellara, Assunta
AU - Coianiz, Nicolò
AU - Franzese, Sara
AU - Frigo, Alessandro
AU - Voza, Antonio
AU - Calcaterra, Francesca
AU - Di Vito, Clara
AU - Della Bella, Silvia
AU - Mikulak, Joanna
AU - Mavilio, Domenico
N1 - © 2024. The Author(s).
PY - 2024/3/20
Y1 - 2024/3/20
N2 - γδ T cells provide rapid cellular immunity against pathogens. Here, we conducted matched single-cell RNA-sequencing and γδ-TCR-sequencing to delineate the molecular changes in γδ T cells during a longitudinal study following mRNA SARS-CoV-2 vaccination. While the first dose of vaccine primes Vδ2 T cells, it is the second administration that significantly boosts their immune response. Specifically, the second vaccination uncovers memory features of Vδ2 T cells, shaped by the induction of AP-1 family transcription factors and characterized by a convergent central memory signature, clonal expansion, and an enhanced effector potential. This temporally distinct effector response of Vδ2 T cells was also confirmed in vitro upon stimulation with SARS-CoV-2 spike-peptides. Indeed, the second challenge triggers a significantly higher production of IFNγ by Vδ2 T cells. Collectively, our findings suggest that mRNA SARS-CoV-2 vaccination might benefit from the establishment of long-lasting central memory Vδ2 T cells to confer protection against SARS-CoV-2 infection.
AB - γδ T cells provide rapid cellular immunity against pathogens. Here, we conducted matched single-cell RNA-sequencing and γδ-TCR-sequencing to delineate the molecular changes in γδ T cells during a longitudinal study following mRNA SARS-CoV-2 vaccination. While the first dose of vaccine primes Vδ2 T cells, it is the second administration that significantly boosts their immune response. Specifically, the second vaccination uncovers memory features of Vδ2 T cells, shaped by the induction of AP-1 family transcription factors and characterized by a convergent central memory signature, clonal expansion, and an enhanced effector potential. This temporally distinct effector response of Vδ2 T cells was also confirmed in vitro upon stimulation with SARS-CoV-2 spike-peptides. Indeed, the second challenge triggers a significantly higher production of IFNγ by Vδ2 T cells. Collectively, our findings suggest that mRNA SARS-CoV-2 vaccination might benefit from the establishment of long-lasting central memory Vδ2 T cells to confer protection against SARS-CoV-2 infection.
U2 - 10.1038/s41541-024-00853-9
DO - 10.1038/s41541-024-00853-9
M3 - SCORING: Journal article
C2 - 38509155
VL - 9
SP - 63
JO - NPJ VACCINES
JF - NPJ VACCINES
SN - 2059-0105
IS - 1
ER -