Even short-time storage in physiological saline solution impairs endothelial vascular function of saphenous vein grafts

TY - JOUR

T1 - Even short-time storage in physiological saline solution impairs endothelial vascular function of saphenous vein grafts

AU - Wilbring, Manuel

AU - Tugtekin, Sems Malte

AU - Zatschler, Birgit

AU - Ebner, Anette

AU - Reichenspurner, Hermann

AU - Matschke, Klaus

AU - Deussen, Andreas

N1 - Published by Elsevier B.V.

PY - 2011/10

Y1 - 2011/10

N2 - OBJECTIVES: A faultless endothelial layer is decisive for vascular function and therewith graft's patency. Functional impairment of the endothelium increases risk of graft thrombosis, intimal hyperplasia, and consecutive accelerated graft atherosclerosis. Storage solutions for intra-operatively harvested saphenous vein segments (SVS) might have significant impact on endothelial function. We investigated the impact of short-time storage in physiological saline solution (PSS) and a potassium-chloride- and N-acetylhistidine-enriched storage solution on venous endothelial function.METHODS: Intra-operatively isolated SVSs (n=19) were stored in different storage solutions for 90 min. They were then immediately studied in tissue bath at 36°C with continuous oxygen insufflation. Following preconstriction with norepinephrine, dose-response relaxation curves of bradykinine (Brad) and sodium nitroprusside (SNP) were determined. We compared developed maximum wall tension, vessel constriction kinetics, endothelial cell- and smooth muscle cell (SMC)-dependent vasodilatory function.RESULTS: Maximum vessel wall tension was reduced significantly in PSS-stored vessels (10.1 ± 9.8 mN mm(-1) vs 3.5 ± 3.4 mN mm(-1); p=0.0372). Endothelium-derived vasodilatory function was likewise significantly reduced after short-time storage (20.6 ± 34.4% vs 35.0 ± 27.0%; p=0.0437). SNP-mediated SMC-vasodilatory function was maintained equally well in both groups (88.2 ± 21.8% vs 83.0 ± 30.6% in PSS; p=n.s.).CONCLUSION: Even short-time storage in PSS significantly impairs endothelial vascular function. Concerning the essential role of a faultless endothelial layer, the quite common use of PSS as a storage solution for SVSs in CABG surgery has to be discussed critically.

AB - OBJECTIVES: A faultless endothelial layer is decisive for vascular function and therewith graft's patency. Functional impairment of the endothelium increases risk of graft thrombosis, intimal hyperplasia, and consecutive accelerated graft atherosclerosis. Storage solutions for intra-operatively harvested saphenous vein segments (SVS) might have significant impact on endothelial function. We investigated the impact of short-time storage in physiological saline solution (PSS) and a potassium-chloride- and N-acetylhistidine-enriched storage solution on venous endothelial function.METHODS: Intra-operatively isolated SVSs (n=19) were stored in different storage solutions for 90 min. They were then immediately studied in tissue bath at 36°C with continuous oxygen insufflation. Following preconstriction with norepinephrine, dose-response relaxation curves of bradykinine (Brad) and sodium nitroprusside (SNP) were determined. We compared developed maximum wall tension, vessel constriction kinetics, endothelial cell- and smooth muscle cell (SMC)-dependent vasodilatory function.RESULTS: Maximum vessel wall tension was reduced significantly in PSS-stored vessels (10.1 ± 9.8 mN mm(-1) vs 3.5 ± 3.4 mN mm(-1); p=0.0372). Endothelium-derived vasodilatory function was likewise significantly reduced after short-time storage (20.6 ± 34.4% vs 35.0 ± 27.0%; p=0.0437). SNP-mediated SMC-vasodilatory function was maintained equally well in both groups (88.2 ± 21.8% vs 83.0 ± 30.6% in PSS; p=n.s.).CONCLUSION: Even short-time storage in PSS significantly impairs endothelial vascular function. Concerning the essential role of a faultless endothelial layer, the quite common use of PSS as a storage solution for SVSs in CABG surgery has to be discussed critically.

KW - Aged

KW - Bradykinin/pharmacology

KW - Coronary Artery Bypass/methods

KW - Dose-Response Relationship, Drug

KW - Endothelium, Vascular/drug effects

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Organ Preservation Solutions/pharmacology

KW - Saphenous Vein/drug effects

KW - Sodium Chloride/pharmacology

KW - Time Factors

KW - Tissue Culture Techniques

KW - Tissue Preservation/methods

KW - Tissue and Organ Harvesting/methods

KW - Vascular Patency/drug effects

KW - Vasodilation/drug effects

KW - Vasodilator Agents/pharmacology

U2 - 10.1016/j.ejcts.2011.01.024

DO - 10.1016/j.ejcts.2011.01.024

M3 - SCORING: Journal article

C2 - 21376620

VL - 40

SP - 811

EP - 815

JO - EUR J CARDIO-THORAC

JF - EUR J CARDIO-THORAC

SN - 1010-7940

IS - 4

ER -