Even short-time storage in physiological saline solution impairs endothelial vascular function of saphenous vein grafts
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Even short-time storage in physiological saline solution impairs endothelial vascular function of saphenous vein grafts. / Wilbring, Manuel; Tugtekin, Sems Malte; Zatschler, Birgit; Ebner, Anette; Reichenspurner, Hermann; Matschke, Klaus; Deussen, Andreas.
in: EUR J CARDIO-THORAC, Jahrgang 40, Nr. 4, 10.2011, S. 811-815.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Even short-time storage in physiological saline solution impairs endothelial vascular function of saphenous vein grafts
AU - Wilbring, Manuel
AU - Tugtekin, Sems Malte
AU - Zatschler, Birgit
AU - Ebner, Anette
AU - Reichenspurner, Hermann
AU - Matschke, Klaus
AU - Deussen, Andreas
N1 - Published by Elsevier B.V.
PY - 2011/10
Y1 - 2011/10
N2 - OBJECTIVES: A faultless endothelial layer is decisive for vascular function and therewith graft's patency. Functional impairment of the endothelium increases risk of graft thrombosis, intimal hyperplasia, and consecutive accelerated graft atherosclerosis. Storage solutions for intra-operatively harvested saphenous vein segments (SVS) might have significant impact on endothelial function. We investigated the impact of short-time storage in physiological saline solution (PSS) and a potassium-chloride- and N-acetylhistidine-enriched storage solution on venous endothelial function.METHODS: Intra-operatively isolated SVSs (n=19) were stored in different storage solutions for 90 min. They were then immediately studied in tissue bath at 36°C with continuous oxygen insufflation. Following preconstriction with norepinephrine, dose-response relaxation curves of bradykinine (Brad) and sodium nitroprusside (SNP) were determined. We compared developed maximum wall tension, vessel constriction kinetics, endothelial cell- and smooth muscle cell (SMC)-dependent vasodilatory function.RESULTS: Maximum vessel wall tension was reduced significantly in PSS-stored vessels (10.1 ± 9.8 mN mm(-1) vs 3.5 ± 3.4 mN mm(-1); p=0.0372). Endothelium-derived vasodilatory function was likewise significantly reduced after short-time storage (20.6 ± 34.4% vs 35.0 ± 27.0%; p=0.0437). SNP-mediated SMC-vasodilatory function was maintained equally well in both groups (88.2 ± 21.8% vs 83.0 ± 30.6% in PSS; p=n.s.).CONCLUSION: Even short-time storage in PSS significantly impairs endothelial vascular function. Concerning the essential role of a faultless endothelial layer, the quite common use of PSS as a storage solution for SVSs in CABG surgery has to be discussed critically.
AB - OBJECTIVES: A faultless endothelial layer is decisive for vascular function and therewith graft's patency. Functional impairment of the endothelium increases risk of graft thrombosis, intimal hyperplasia, and consecutive accelerated graft atherosclerosis. Storage solutions for intra-operatively harvested saphenous vein segments (SVS) might have significant impact on endothelial function. We investigated the impact of short-time storage in physiological saline solution (PSS) and a potassium-chloride- and N-acetylhistidine-enriched storage solution on venous endothelial function.METHODS: Intra-operatively isolated SVSs (n=19) were stored in different storage solutions for 90 min. They were then immediately studied in tissue bath at 36°C with continuous oxygen insufflation. Following preconstriction with norepinephrine, dose-response relaxation curves of bradykinine (Brad) and sodium nitroprusside (SNP) were determined. We compared developed maximum wall tension, vessel constriction kinetics, endothelial cell- and smooth muscle cell (SMC)-dependent vasodilatory function.RESULTS: Maximum vessel wall tension was reduced significantly in PSS-stored vessels (10.1 ± 9.8 mN mm(-1) vs 3.5 ± 3.4 mN mm(-1); p=0.0372). Endothelium-derived vasodilatory function was likewise significantly reduced after short-time storage (20.6 ± 34.4% vs 35.0 ± 27.0%; p=0.0437). SNP-mediated SMC-vasodilatory function was maintained equally well in both groups (88.2 ± 21.8% vs 83.0 ± 30.6% in PSS; p=n.s.).CONCLUSION: Even short-time storage in PSS significantly impairs endothelial vascular function. Concerning the essential role of a faultless endothelial layer, the quite common use of PSS as a storage solution for SVSs in CABG surgery has to be discussed critically.
KW - Aged
KW - Bradykinin/pharmacology
KW - Coronary Artery Bypass/methods
KW - Dose-Response Relationship, Drug
KW - Endothelium, Vascular/drug effects
KW - Female
KW - Humans
KW - Male
KW - Middle Aged
KW - Organ Preservation Solutions/pharmacology
KW - Saphenous Vein/drug effects
KW - Sodium Chloride/pharmacology
KW - Time Factors
KW - Tissue Culture Techniques
KW - Tissue Preservation/methods
KW - Tissue and Organ Harvesting/methods
KW - Vascular Patency/drug effects
KW - Vasodilation/drug effects
KW - Vasodilator Agents/pharmacology
U2 - 10.1016/j.ejcts.2011.01.024
DO - 10.1016/j.ejcts.2011.01.024
M3 - SCORING: Journal article
C2 - 21376620
VL - 40
SP - 811
EP - 815
JO - EUR J CARDIO-THORAC
JF - EUR J CARDIO-THORAC
SN - 1010-7940
IS - 4
ER -