Enzymatic activity of CD26 (dipeptidylpeptidase IV) is not required for its signalling function in T cells
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Enzymatic activity of CD26 (dipeptidylpeptidase IV) is not required for its signalling function in T cells. / Hegen, M; Mittrücker, H W; Hug, R; Demuth, H U; Neubert, K; Barth, A; Fleischer, B.
In: IMMUNOBIOLOGY, Vol. 189, No. 5, 01.12.1993, p. 483-93.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Enzymatic activity of CD26 (dipeptidylpeptidase IV) is not required for its signalling function in T cells
AU - Hegen, M
AU - Mittrücker, H W
AU - Hug, R
AU - Demuth, H U
AU - Neubert, K
AU - Barth, A
AU - Fleischer, B
PY - 1993/12/1
Y1 - 1993/12/1
N2 - CD26 is a proteolytic enzyme (dipeptidylpeptidase IV) expressed on the T cell surface that defines an alternative activation signal for human T lymphocytes. Crosslinking of CD26 via monoclonal antibodies triggers proliferation and cytotoxicity in preactivated T cells. In this study, we used highly specific competitive and irreversible inhibitors of dipeptidylpeptidase IV to study the role of the enzymatic activity in activation of CD26-transfected T cells as well as of CD26-expressing normal human T cell clones. These inhibitors at concentrations that blocked up to 95% of the enzymatic activity, did not specifically inhibit T cell activation neither via TCR/CD3 nor via CD26 itself. This demonstrates that the enzymatic activity of CD26 is not required for its T cell activating properties.
AB - CD26 is a proteolytic enzyme (dipeptidylpeptidase IV) expressed on the T cell surface that defines an alternative activation signal for human T lymphocytes. Crosslinking of CD26 via monoclonal antibodies triggers proliferation and cytotoxicity in preactivated T cells. In this study, we used highly specific competitive and irreversible inhibitors of dipeptidylpeptidase IV to study the role of the enzymatic activity in activation of CD26-transfected T cells as well as of CD26-expressing normal human T cell clones. These inhibitors at concentrations that blocked up to 95% of the enzymatic activity, did not specifically inhibit T cell activation neither via TCR/CD3 nor via CD26 itself. This demonstrates that the enzymatic activity of CD26 is not required for its T cell activating properties.
KW - Animals
KW - Antigens, Differentiation, T-Lymphocyte
KW - Cell Line
KW - Cytotoxicity Tests, Immunologic
KW - Dipeptidyl Peptidase 4
KW - Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
KW - Humans
KW - Interleukin-2
KW - Lymphocyte Activation
KW - Mice
KW - Signal Transduction
KW - T-Lymphocytes
KW - Tumor Cells, Cultured
U2 - 10.1016/S0171-2985(11)80419-5
DO - 10.1016/S0171-2985(11)80419-5
M3 - SCORING: Journal article
C2 - 7907318
VL - 189
SP - 483
EP - 493
JO - IMMUNOBIOLOGY
JF - IMMUNOBIOLOGY
SN - 0171-2985
IS - 5
ER -