Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells

Heba Al-Zer; Christian Apel; Max Heiland; Reinhard E Friedrich; Ole Jung; Nadja Kroeger; Wolfgang Eichhorn; Ralf Smeets

Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells

Standard

Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells. / Al-Zer, Heba; Apel, Christian; Heiland, Max; Friedrich, Reinhard E; Jung, Ole; Kroeger, Nadja; Eichhorn, Wolfgang; Smeets, Ralf.

In: IN VIVO, Vol. 29, No. 3, 16.05.2015, p. 319-326.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Al-Zer, H, Apel, C, Heiland, M, Friedrich, RE, Jung, O, Kroeger, N, Eichhorn, W & Smeets, R 2015, 'Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells', IN VIVO, vol. 29, no. 3, pp. 319-326.

APA

Al-Zer, H., Apel, C., Heiland, M., Friedrich, R. E., Jung, O., Kroeger, N., Eichhorn, W., & Smeets, R. (2015). Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells. IN VIVO, 29(3), 319-326.

Vancouver

Al-Zer H, Apel C, Heiland M, Friedrich RE, Jung O, Kroeger N et al. Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells. IN VIVO. 2015 May 16;29(3):319-326.

Bibtex

@article{06278c705aeb4bf3a6534c713a8f0356,

title = "Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells",

abstract = "BACKGROUND/AIM: As already described in previous studies, neural crest stem cells (NCSCs) can be found in adult human dental pulp. The present study investigated the methodology for enrichment and differentiation-induction of the above mentioned cells.MATERIALS AND METHODS: Dental pulp was extracted from human wisdom teeth of four patients and subsequently cultured as explants on fibronectin-coated plates in neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin, l-glutamine and neuregulin-β1. The cells were then characterized by immunofluorescence, while their differentiation-potential was tested by the attempt to induce cells into different lineages, i.e. osteogenic, melanocytic and glial.RESULTS: The enriched cell population expressed nestin, CD271 and SOX10, which are well-known markers for NCSCs. Consequently, the cells were successfully induced to differentiate into osteoblasts, melanocytes and Schwann cells, expressing the corresponding differentiation markers.CONCLUSION: Human adult dental pulp contains a population of stem cells with neural crest ontogeny, which can thus be recruited for multiple regenerative therapies.",

author = "Heba Al-Zer and Christian Apel and Max Heiland and Friedrich, {Reinhard E} and Ole Jung and Nadja Kroeger and Wolfgang Eichhorn and Ralf Smeets",

year = "2015",

month = may,

day = "16",

language = "English",

volume = "29",

pages = "319--326",

journal = "IN VIVO",

issn = "0258-851X",

publisher = "International Institute of Anticancer Research",

number = "3",

}

RIS

TY - JOUR

T1 - Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells

AU - Al-Zer, Heba

AU - Apel, Christian

AU - Heiland, Max

AU - Friedrich, Reinhard E

AU - Jung, Ole

AU - Kroeger, Nadja

AU - Eichhorn, Wolfgang

AU - Smeets, Ralf

PY - 2015/5/16

Y1 - 2015/5/16

N2 - BACKGROUND/AIM: As already described in previous studies, neural crest stem cells (NCSCs) can be found in adult human dental pulp. The present study investigated the methodology for enrichment and differentiation-induction of the above mentioned cells.MATERIALS AND METHODS: Dental pulp was extracted from human wisdom teeth of four patients and subsequently cultured as explants on fibronectin-coated plates in neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin, l-glutamine and neuregulin-β1. The cells were then characterized by immunofluorescence, while their differentiation-potential was tested by the attempt to induce cells into different lineages, i.e. osteogenic, melanocytic and glial.RESULTS: The enriched cell population expressed nestin, CD271 and SOX10, which are well-known markers for NCSCs. Consequently, the cells were successfully induced to differentiate into osteoblasts, melanocytes and Schwann cells, expressing the corresponding differentiation markers.CONCLUSION: Human adult dental pulp contains a population of stem cells with neural crest ontogeny, which can thus be recruited for multiple regenerative therapies.

AB - BACKGROUND/AIM: As already described in previous studies, neural crest stem cells (NCSCs) can be found in adult human dental pulp. The present study investigated the methodology for enrichment and differentiation-induction of the above mentioned cells.MATERIALS AND METHODS: Dental pulp was extracted from human wisdom teeth of four patients and subsequently cultured as explants on fibronectin-coated plates in neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin, l-glutamine and neuregulin-β1. The cells were then characterized by immunofluorescence, while their differentiation-potential was tested by the attempt to induce cells into different lineages, i.e. osteogenic, melanocytic and glial.RESULTS: The enriched cell population expressed nestin, CD271 and SOX10, which are well-known markers for NCSCs. Consequently, the cells were successfully induced to differentiate into osteoblasts, melanocytes and Schwann cells, expressing the corresponding differentiation markers.CONCLUSION: Human adult dental pulp contains a population of stem cells with neural crest ontogeny, which can thus be recruited for multiple regenerative therapies.

M3 - SCORING: Journal article

C2 - 25977377

VL - 29

SP - 319

EP - 326

JO - IN VIVO

JF - IN VIVO

SN - 0258-851X

IS - 3

ER -