Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells
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Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells. / Al-Zer, Heba; Apel, Christian; Heiland, Max; Friedrich, Reinhard E; Jung, Ole; Kroeger, Nadja; Eichhorn, Wolfgang; Smeets, Ralf.
in: IN VIVO, Jahrgang 29, Nr. 3, 16.05.2015, S. 319-326.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Enrichment and Schwann Cell Differentiation of Neural Crest-derived Dental Pulp Stem Cells
AU - Al-Zer, Heba
AU - Apel, Christian
AU - Heiland, Max
AU - Friedrich, Reinhard E
AU - Jung, Ole
AU - Kroeger, Nadja
AU - Eichhorn, Wolfgang
AU - Smeets, Ralf
N1 - Copyright © 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
PY - 2015/5/16
Y1 - 2015/5/16
N2 - BACKGROUND/AIM: As already described in previous studies, neural crest stem cells (NCSCs) can be found in adult human dental pulp. The present study investigated the methodology for enrichment and differentiation-induction of the above mentioned cells.MATERIALS AND METHODS: Dental pulp was extracted from human wisdom teeth of four patients and subsequently cultured as explants on fibronectin-coated plates in neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin, l-glutamine and neuregulin-β1. The cells were then characterized by immunofluorescence, while their differentiation-potential was tested by the attempt to induce cells into different lineages, i.e. osteogenic, melanocytic and glial.RESULTS: The enriched cell population expressed nestin, CD271 and SOX10, which are well-known markers for NCSCs. Consequently, the cells were successfully induced to differentiate into osteoblasts, melanocytes and Schwann cells, expressing the corresponding differentiation markers.CONCLUSION: Human adult dental pulp contains a population of stem cells with neural crest ontogeny, which can thus be recruited for multiple regenerative therapies.
AB - BACKGROUND/AIM: As already described in previous studies, neural crest stem cells (NCSCs) can be found in adult human dental pulp. The present study investigated the methodology for enrichment and differentiation-induction of the above mentioned cells.MATERIALS AND METHODS: Dental pulp was extracted from human wisdom teeth of four patients and subsequently cultured as explants on fibronectin-coated plates in neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin, l-glutamine and neuregulin-β1. The cells were then characterized by immunofluorescence, while their differentiation-potential was tested by the attempt to induce cells into different lineages, i.e. osteogenic, melanocytic and glial.RESULTS: The enriched cell population expressed nestin, CD271 and SOX10, which are well-known markers for NCSCs. Consequently, the cells were successfully induced to differentiate into osteoblasts, melanocytes and Schwann cells, expressing the corresponding differentiation markers.CONCLUSION: Human adult dental pulp contains a population of stem cells with neural crest ontogeny, which can thus be recruited for multiple regenerative therapies.
M3 - SCORING: Journal article
C2 - 25977377
VL - 29
SP - 319
EP - 326
JO - IN VIVO
JF - IN VIVO
SN - 0258-851X
IS - 3
ER -