Endothelin-1 mRNA and protein in vascular wall cells is increased by reactive oxygen species
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Endothelin-1 mRNA and protein in vascular wall cells is increased by reactive oxygen species. / Kaehler, Jan; Sill, Bjoern; Koester, Ralf; Mittmann, Clemens; Orzechowski, Hans-Dieter; Muenzel, Thomas; Meinertz, Thomas.
In: CLIN SCI, Vol. 103, No. Suppl 48, 08.2002, p. 176S-178S.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Endothelin-1 mRNA and protein in vascular wall cells is increased by reactive oxygen species
AU - Kaehler, Jan
AU - Sill, Bjoern
AU - Koester, Ralf
AU - Mittmann, Clemens
AU - Orzechowski, Hans-Dieter
AU - Muenzel, Thomas
AU - Meinertz, Thomas
PY - 2002/8
Y1 - 2002/8
N2 - A dysregulated metabolism of oxygen-derived free radicals, nitric oxide and endothelin-1(ET-1) in conditions such as hypercholesterolaemia or hypertension may promote the development of atherosclerosis. We therefore subjected cultured human umbilical vein endothelial cells and coronary artery smooth muscle cells to oxidative stress induced by xanthine oxidase or hydrogen peroxide and observed alterations in ET-1 metabolism. Incubation with oxygen-derived free radicals increased preproET-1 promoter activity, ET-1 mRNA synthesis and big ET-1 concentrations in both cell types. This interaction of oxidative stress and ET-1 expression may be relevant in atherogenic conditions such as hypercholesterolaemia and hypertension since our data indicate that oxidative stress further aggravates the injurious effects attributed to ET-1.
AB - A dysregulated metabolism of oxygen-derived free radicals, nitric oxide and endothelin-1(ET-1) in conditions such as hypercholesterolaemia or hypertension may promote the development of atherosclerosis. We therefore subjected cultured human umbilical vein endothelial cells and coronary artery smooth muscle cells to oxidative stress induced by xanthine oxidase or hydrogen peroxide and observed alterations in ET-1 metabolism. Incubation with oxygen-derived free radicals increased preproET-1 promoter activity, ET-1 mRNA synthesis and big ET-1 concentrations in both cell types. This interaction of oxidative stress and ET-1 expression may be relevant in atherogenic conditions such as hypercholesterolaemia and hypertension since our data indicate that oxidative stress further aggravates the injurious effects attributed to ET-1.
KW - Cells, Cultured
KW - Coronary Vessels
KW - Endothelin-1/genetics
KW - Endothelins/metabolism
KW - Endothelium, Vascular/metabolism
KW - Humans
KW - Hydrogen Peroxide/pharmacology
KW - Muscle, Smooth, Vascular/metabolism
KW - Protein Precursors/metabolism
KW - RNA, Messenger/metabolism
KW - Reactive Oxygen Species/pharmacology
KW - Stimulation, Chemical
KW - Umbilical Veins
KW - Xanthine Oxidase/pharmacology
U2 - 10.1042/CS103S176S
DO - 10.1042/CS103S176S
M3 - SCORING: Journal article
C2 - 12193080
VL - 103
SP - 176S-178S
JO - CLIN SCI
JF - CLIN SCI
SN - 0143-5221
IS - Suppl 48
ER -