Endocrine controls of primary adult human stem cell biology: thyroid hormones stimulate keratin 15 expression, apoptosis, and differentiation in human hair follicle epithelial stem cells in situ and in vitro
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Endocrine controls of primary adult human stem cell biology: thyroid hormones stimulate keratin 15 expression, apoptosis, and differentiation in human hair follicle epithelial stem cells in situ and in vitro. / Tiede, S; Bohm, K; Meier, N; Funk, W; Paus, R.
In: EUR J CELL BIOL, Vol. 89, No. 10, 10.2010, p. 769-77.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Endocrine controls of primary adult human stem cell biology: thyroid hormones stimulate keratin 15 expression, apoptosis, and differentiation in human hair follicle epithelial stem cells in situ and in vitro
AU - Tiede, S
AU - Bohm, K
AU - Meier, N
AU - Funk, W
AU - Paus, R
N1 - Copyright (c) 2010 Elsevier GmbH. All rights reserved.
PY - 2010/10
Y1 - 2010/10
N2 - Here we demonstrate that physiological concentrations of the thyroid hormones T3 and T4 enhance the KERATIN 15 promoter activity and expression in epithelial stem cells of adult human scalp hair follicles in situ and in vitro. Additionally, T3 and T4 stimulate expression of the immuno-inhibitory surface molecule CD200. Subsequently, T3 and T4 induce apoptosis and differentiation and inhibit clonal growth of these progenitor cells in vitro. These data suggest that human hair follicle bulge-derived epithelial stem cells underlie profound, previously unknown hormonal regulation by thyroid hormones, and show that primary human keratin 15-GFP+ progenitor cells can be exploited to further elucidate fundamental endocrine controls of human epithelial stem cells.
AB - Here we demonstrate that physiological concentrations of the thyroid hormones T3 and T4 enhance the KERATIN 15 promoter activity and expression in epithelial stem cells of adult human scalp hair follicles in situ and in vitro. Additionally, T3 and T4 stimulate expression of the immuno-inhibitory surface molecule CD200. Subsequently, T3 and T4 induce apoptosis and differentiation and inhibit clonal growth of these progenitor cells in vitro. These data suggest that human hair follicle bulge-derived epithelial stem cells underlie profound, previously unknown hormonal regulation by thyroid hormones, and show that primary human keratin 15-GFP+ progenitor cells can be exploited to further elucidate fundamental endocrine controls of human epithelial stem cells.
KW - Adult
KW - Apoptosis
KW - Cell Differentiation
KW - Cell Line
KW - Cell Proliferation
KW - Cells, Cultured
KW - Epithelial Cells
KW - Hair Follicle
KW - Humans
KW - Keratin-15
KW - Organ Culture Techniques
KW - Stem Cells
KW - Thyroxine
KW - Triiodothyronine
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.ejcb.2010.06.002
DO - 10.1016/j.ejcb.2010.06.002
M3 - SCORING: Journal article
C2 - 20656376
VL - 89
SP - 769
EP - 777
JO - EUR J CELL BIOL
JF - EUR J CELL BIOL
SN - 0171-9335
IS - 10
ER -