Emergence of virus escape mutants after immunization with epitope vaccine
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Emergence of virus escape mutants after immunization with epitope vaccine. / Weidt, G; Deppert, W; Utermöhlen, O; Heukeshoven, J; Lehmann-Grube, F.
In: J VIROL, Vol. 69, No. 11, 11.1995, p. 7147-51.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Emergence of virus escape mutants after immunization with epitope vaccine
AU - Weidt, G
AU - Deppert, W
AU - Utermöhlen, O
AU - Heukeshoven, J
AU - Lehmann-Grube, F
PY - 1995/11
Y1 - 1995/11
N2 - BALB/c and C57BL/6J mice were immunized with recombinant vaccines consisting of lymphocytic choriomeningitis virus CD8+ T-lymphocyte epitopes and a carrier protein. During challenge infection with WE strain lymphocytic choriomeningitis virus, mutants with alterations in distinct amino acid residues of the epitopic nonapeptides appeared and multiplied. Splenocytes from WE-infected BALB/c mice lysed cells coated with the WE-type epitope; lysis was considerably less effective when the epitopic nonapeptide with which the syngeneic cells had been sensitized was the mutated form. Neither target was lysed by splenocytes from BALB/c mice infected with the variant virus. Mutants were not detected in F1 hybrid mice immunized with two viral epitopes that were restricted by class I molecules of both parents.
AB - BALB/c and C57BL/6J mice were immunized with recombinant vaccines consisting of lymphocytic choriomeningitis virus CD8+ T-lymphocyte epitopes and a carrier protein. During challenge infection with WE strain lymphocytic choriomeningitis virus, mutants with alterations in distinct amino acid residues of the epitopic nonapeptides appeared and multiplied. Splenocytes from WE-infected BALB/c mice lysed cells coated with the WE-type epitope; lysis was considerably less effective when the epitopic nonapeptide with which the syngeneic cells had been sensitized was the mutated form. Neither target was lysed by splenocytes from BALB/c mice infected with the variant virus. Mutants were not detected in F1 hybrid mice immunized with two viral epitopes that were restricted by class I molecules of both parents.
KW - Amino Acid Sequence
KW - Animals
KW - Base Sequence
KW - CD8-Positive T-Lymphocytes
KW - Capsid
KW - DNA Primers
KW - DNA, Complementary
KW - DNA, Viral
KW - Epitopes
KW - Female
KW - Lymphocytic choriomeningitis virus
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, Inbred C57BL
KW - Mice, Inbred Strains
KW - Molecular Sequence Data
KW - Mutagenesis
KW - Polymerase Chain Reaction
KW - Vaccines, Synthetic
KW - Viral Core Proteins
KW - Viral Vaccines
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 7474135
VL - 69
SP - 7147
EP - 7151
JO - J VIROL
JF - J VIROL
SN - 0022-538X
IS - 11
ER -