Egfr amplification specific gene expression in phyllodes tumours of the breast.
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Egfr amplification specific gene expression in phyllodes tumours of the breast. / Agelopoulos, Konstantin; Kersting, Christian; Korsching, Eberhard; Schmidt, Hartmut; Kuijper, Arno; August, Christian; Wülfing, Pia; Tio, Joke; Boecker, Werner; Diest, van; Brandt, Burkhard; Brandt, Burkhard; Buerger, Horst.
In: MOL CELL ONCOL, Vol. 29, No. 6, 6, 2007, p. 443-451.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Egfr amplification specific gene expression in phyllodes tumours of the breast.
AU - Agelopoulos, Konstantin
AU - Kersting, Christian
AU - Korsching, Eberhard
AU - Schmidt, Hartmut
AU - Kuijper, Arno
AU - August, Christian
AU - Wülfing, Pia
AU - Tio, Joke
AU - Boecker, Werner
AU - Diest, van
AU - Brandt, Burkhard
AU - Brandt, Burkhard
AU - Buerger, Horst
PY - 2007
Y1 - 2007
N2 - BACKGROUND: Recently, we were able to show that amplifications of the epidermal growth factor receptor (egfr) gene and the overexpression of EGFR were associated with the initiation and progression of phyllodes tumours. METHODS: In order to gain further insights into regulation mechanisms associated with egfr amplifications and EGFR expression in phyllodes tumours, we performed global gene expression analysis (Affymetrix A133.2) on a series of 10 phyllodes tumours, of these three with and seven without amplifications of an important regulatory repeat in intron 1 of egfr (CA-SSR I). The results were verified and extended by means of immunohistochemistry using the tissue microarray method on an extensively characterized series of 58 phyllodes tumours with antibodies against caveolin-1, eps15, EGF, TGF-alpha, pErk, pAkt and mdm2. RESULTS: We were able to show that the presence of egfr CA-SSR I amplifications in phyllodes tumours was associated with 230 differentially expressed genes. Caveolin-1 and eps15, involved in EGFR turnover and signalling, were regulated differentially on the RNA and protein level proportionally to egfr gene dosage. Further immunohistochemical analysis revealed that the expression of caveolin-1 and eps15 were also significantly correlated with the expression of pAkt (p
AB - BACKGROUND: Recently, we were able to show that amplifications of the epidermal growth factor receptor (egfr) gene and the overexpression of EGFR were associated with the initiation and progression of phyllodes tumours. METHODS: In order to gain further insights into regulation mechanisms associated with egfr amplifications and EGFR expression in phyllodes tumours, we performed global gene expression analysis (Affymetrix A133.2) on a series of 10 phyllodes tumours, of these three with and seven without amplifications of an important regulatory repeat in intron 1 of egfr (CA-SSR I). The results were verified and extended by means of immunohistochemistry using the tissue microarray method on an extensively characterized series of 58 phyllodes tumours with antibodies against caveolin-1, eps15, EGF, TGF-alpha, pErk, pAkt and mdm2. RESULTS: We were able to show that the presence of egfr CA-SSR I amplifications in phyllodes tumours was associated with 230 differentially expressed genes. Caveolin-1 and eps15, involved in EGFR turnover and signalling, were regulated differentially on the RNA and protein level proportionally to egfr gene dosage. Further immunohistochemical analysis revealed that the expression of caveolin-1 and eps15 were also significantly correlated with the expression of pAkt (p
U2 - 10.1155/2007/754712
DO - 10.1155/2007/754712
M3 - SCORING: Zeitschriftenaufsatz
VL - 29
SP - 443
EP - 451
JO - MOL CELL ONCOL
JF - MOL CELL ONCOL
SN - 2372-3556
IS - 6
M1 - 6
ER -