Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.

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Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms. / Beck-Bornholdt, Hans-Peter.

In: Cell Tissue Kinet, Vol. 10, No. 3, 3, 1977, p. 265-268.

Research output: SCORING: Contribution to journalSCORING: Journal articleResearchpeer-review

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Beck-Bornholdt, H-P 1977, 'Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.', Cell Tissue Kinet, vol. 10, no. 3, 3, pp. 265-268. https://doi.org/10.1111/j.1365-2184.1977.tb00294.x

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Bibtex

@article{41cbeba28721486089e8e0a682d5accc,
title = "Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.",
abstract = "Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.",
author = "Hans-Peter Beck-Bornholdt",
year = "1977",
doi = "10.1111/j.1365-2184.1977.tb00294.x",
language = "Deutsch",
volume = "10",
pages = "265--268",
number = "3",

}

RIS

TY - JOUR

T1 - Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.

AU - Beck-Bornholdt, Hans-Peter

PY - 1977

Y1 - 1977

N2 - Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.

AB - Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.

U2 - 10.1111/j.1365-2184.1977.tb00294.x

DO - 10.1111/j.1365-2184.1977.tb00294.x

M3 - SCORING: Zeitschriftenaufsatz

VL - 10

SP - 265

EP - 268

IS - 3

M1 - 3

ER -