Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.
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Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms. / Beck-Bornholdt, Hans-Peter.
in: Cell Tissue Kinet, Jahrgang 10, Nr. 3, 3, 1977, S. 265-268.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.
AU - Beck-Bornholdt, Hans-Peter
PY - 1977
Y1 - 1977
N2 - Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.
AB - Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.
U2 - 10.1111/j.1365-2184.1977.tb00294.x
DO - 10.1111/j.1365-2184.1977.tb00294.x
M3 - SCORING: Zeitschriftenaufsatz
VL - 10
SP - 265
EP - 268
IS - 3
M1 - 3
ER -