Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes.

Standard

Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes. / Richter, W; Eiermann, Thomas; Scherbaum, W A.

In: HYBRIDOMA, Vol. 9, No. 1, 1, 1990, p. 1-8.

Research output: SCORING: Contribution to journalSCORING: Journal articleResearchpeer-review

Harvard

APA

Vancouver

Richter W, Eiermann T, Scherbaum WA. Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes. HYBRIDOMA. 1990;9(1):1-8. 1.

Bibtex

@article{36f6cc8052b14f4fa8ffc19edc349db2,
title = "Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes.",
abstract = "Plating efficiencies of EBV transformed human B cells seeded in single cell cultures are far lower (less than 1%) than observed in T cell cloning experiments. This report describes the stimulatory effect of several crude as well as recombinant growth factors on proliferation of EBV transformed B cells measured by [3H]thymidine uptake. Supernatant of LPS activated monocytes (HSF) and recombinant interleukin 4 (rIL-4), but not recombinant IL-1 beta, IL-2, IL-6, TNF alpha, GM-CSF, and interferon gamma increased [3H]thymidine incorporation. The combination of HSF and rIL-4 was found to be synergistic on B cell proliferation. Plating efficiency of EBV transformed B cells at limiting dilution was improved by HSF, but not by the combination of HSF and rIL-4.",
author = "W Richter and Thomas Eiermann and Scherbaum, {W A}",
year = "1990",
language = "Deutsch",
volume = "9",
pages = "1--8",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes.

AU - Richter, W

AU - Eiermann, Thomas

AU - Scherbaum, W A

PY - 1990

Y1 - 1990

N2 - Plating efficiencies of EBV transformed human B cells seeded in single cell cultures are far lower (less than 1%) than observed in T cell cloning experiments. This report describes the stimulatory effect of several crude as well as recombinant growth factors on proliferation of EBV transformed B cells measured by [3H]thymidine uptake. Supernatant of LPS activated monocytes (HSF) and recombinant interleukin 4 (rIL-4), but not recombinant IL-1 beta, IL-2, IL-6, TNF alpha, GM-CSF, and interferon gamma increased [3H]thymidine incorporation. The combination of HSF and rIL-4 was found to be synergistic on B cell proliferation. Plating efficiency of EBV transformed B cells at limiting dilution was improved by HSF, but not by the combination of HSF and rIL-4.

AB - Plating efficiencies of EBV transformed human B cells seeded in single cell cultures are far lower (less than 1%) than observed in T cell cloning experiments. This report describes the stimulatory effect of several crude as well as recombinant growth factors on proliferation of EBV transformed B cells measured by [3H]thymidine uptake. Supernatant of LPS activated monocytes (HSF) and recombinant interleukin 4 (rIL-4), but not recombinant IL-1 beta, IL-2, IL-6, TNF alpha, GM-CSF, and interferon gamma increased [3H]thymidine incorporation. The combination of HSF and rIL-4 was found to be synergistic on B cell proliferation. Plating efficiency of EBV transformed B cells at limiting dilution was improved by HSF, but not by the combination of HSF and rIL-4.

M3 - SCORING: Zeitschriftenaufsatz

VL - 9

SP - 1

EP - 8

IS - 1

M1 - 1

ER -