Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes.
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Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes. / Richter, W; Eiermann, Thomas; Scherbaum, W A.
in: HYBRIDOMA, Jahrgang 9, Nr. 1, 1, 1990, S. 1-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes.
AU - Richter, W
AU - Eiermann, Thomas
AU - Scherbaum, W A
PY - 1990
Y1 - 1990
N2 - Plating efficiencies of EBV transformed human B cells seeded in single cell cultures are far lower (less than 1%) than observed in T cell cloning experiments. This report describes the stimulatory effect of several crude as well as recombinant growth factors on proliferation of EBV transformed B cells measured by [3H]thymidine uptake. Supernatant of LPS activated monocytes (HSF) and recombinant interleukin 4 (rIL-4), but not recombinant IL-1 beta, IL-2, IL-6, TNF alpha, GM-CSF, and interferon gamma increased [3H]thymidine incorporation. The combination of HSF and rIL-4 was found to be synergistic on B cell proliferation. Plating efficiency of EBV transformed B cells at limiting dilution was improved by HSF, but not by the combination of HSF and rIL-4.
AB - Plating efficiencies of EBV transformed human B cells seeded in single cell cultures are far lower (less than 1%) than observed in T cell cloning experiments. This report describes the stimulatory effect of several crude as well as recombinant growth factors on proliferation of EBV transformed B cells measured by [3H]thymidine uptake. Supernatant of LPS activated monocytes (HSF) and recombinant interleukin 4 (rIL-4), but not recombinant IL-1 beta, IL-2, IL-6, TNF alpha, GM-CSF, and interferon gamma increased [3H]thymidine incorporation. The combination of HSF and rIL-4 was found to be synergistic on B cell proliferation. Plating efficiency of EBV transformed B cells at limiting dilution was improved by HSF, but not by the combination of HSF and rIL-4.
M3 - SCORING: Zeitschriftenaufsatz
VL - 9
SP - 1
EP - 8
IS - 1
M1 - 1
ER -