Effect of Citrus bergamia juice on human neuroblastoma cells in vitro and in metastatic xenograft models
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Effect of Citrus bergamia juice on human neuroblastoma cells in vitro and in metastatic xenograft models. / Navarra, M; Ursino, M R; Ferlazzo, N; Russo, M; Schumacher, U; Valentiner, U.
In: FITOTERAPIA, Vol. 95, 01.06.2014, p. 83-92.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of Citrus bergamia juice on human neuroblastoma cells in vitro and in metastatic xenograft models
AU - Navarra, M
AU - Ursino, M R
AU - Ferlazzo, N
AU - Russo, M
AU - Schumacher, U
AU - Valentiner, U
N1 - Copyright © 2014. Published by Elsevier B.V.
PY - 2014/6/1
Y1 - 2014/6/1
N2 - Neuroblastoma is the most common extracranial pediatric solid tumor with poor prognosis in children with disseminated stage of disease. A number of studies show that molecules largely distributed in commonly consumed fruits and vegetables may have anti-tumor activity. In this study we evaluate the effect of Citrus bergamia (bergamot) juice (BJ) in vitro and in a spontaneous metastatic neuroblastoma SCID mouse model. Qualitative and quantitative characterizations of BJ flavonoid fractions were performed by RP-HPLC/PDA/MS. We show that BJ significantly affects SK-N-SH and LAN-1 cell proliferation in vitro, but fails to reduce primary tumor weight in vivo. Moreover, BJ reduced cell adhesiveness and invasion of LAN-1 and SK-N-SH cells in vitro and the number of pulmonary metastases under consideration of the number of tumor cells in the blood in mice inoculated with LAN-1 cells in vivo. These effects without any apparent sign of systemic toxicity confirm the potential clinical interest of BJ and lay the basis for further investigation in cancer.
AB - Neuroblastoma is the most common extracranial pediatric solid tumor with poor prognosis in children with disseminated stage of disease. A number of studies show that molecules largely distributed in commonly consumed fruits and vegetables may have anti-tumor activity. In this study we evaluate the effect of Citrus bergamia (bergamot) juice (BJ) in vitro and in a spontaneous metastatic neuroblastoma SCID mouse model. Qualitative and quantitative characterizations of BJ flavonoid fractions were performed by RP-HPLC/PDA/MS. We show that BJ significantly affects SK-N-SH and LAN-1 cell proliferation in vitro, but fails to reduce primary tumor weight in vivo. Moreover, BJ reduced cell adhesiveness and invasion of LAN-1 and SK-N-SH cells in vitro and the number of pulmonary metastases under consideration of the number of tumor cells in the blood in mice inoculated with LAN-1 cells in vivo. These effects without any apparent sign of systemic toxicity confirm the potential clinical interest of BJ and lay the basis for further investigation in cancer.
U2 - 10.1016/j.fitote.2014.02.009
DO - 10.1016/j.fitote.2014.02.009
M3 - SCORING: Journal article
C2 - 24594241
VL - 95
SP - 83
EP - 92
JO - FITOTERAPIA
JF - FITOTERAPIA
SN - 0367-326X
ER -