EBV renders B cells susceptible to HIV-1 in humanized mice
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EBV renders B cells susceptible to HIV-1 in humanized mice. / McHugh, Donal; Myburgh, Renier; Caduff, Nicole; Spohn, Michael; Kok, Yik Lim; Keller, Christian W; Murer, Anita; Chatterjee, Bithi; Rühl, Julia; Engelmann, Christine; Chijioke, Obinna; Quast, Isaak; Shilaih, Mohaned; Strouvelle, Victoria P; Neumann, Kathrin; Menter, Thomas; Dirnhofer, Stephan; Lam, Janice Kp; Hui, Kwai F; Bredl, Simon; Schlaepfer, Erika; Sorce, Silvia; Zbinden, Andrea; Capaul, Riccarda; Lünemann, Jan D; Aguzzi, Adriano; Chiang, Alan Ks; Kempf, Werner; Trkola, Alexandra; Metzner, Karin J; Manz, Markus G; Grundhoff, Adam; Speck, Roberto F; Münz, Christian.
In: LIFE SCI ALLIANCE, Vol. 3, No. 8, 08.2020.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - EBV renders B cells susceptible to HIV-1 in humanized mice
AU - McHugh, Donal
AU - Myburgh, Renier
AU - Caduff, Nicole
AU - Spohn, Michael
AU - Kok, Yik Lim
AU - Keller, Christian W
AU - Murer, Anita
AU - Chatterjee, Bithi
AU - Rühl, Julia
AU - Engelmann, Christine
AU - Chijioke, Obinna
AU - Quast, Isaak
AU - Shilaih, Mohaned
AU - Strouvelle, Victoria P
AU - Neumann, Kathrin
AU - Menter, Thomas
AU - Dirnhofer, Stephan
AU - Lam, Janice Kp
AU - Hui, Kwai F
AU - Bredl, Simon
AU - Schlaepfer, Erika
AU - Sorce, Silvia
AU - Zbinden, Andrea
AU - Capaul, Riccarda
AU - Lünemann, Jan D
AU - Aguzzi, Adriano
AU - Chiang, Alan Ks
AU - Kempf, Werner
AU - Trkola, Alexandra
AU - Metzner, Karin J
AU - Manz, Markus G
AU - Grundhoff, Adam
AU - Speck, Roberto F
AU - Münz, Christian
N1 - © 2020 McHugh et al.
PY - 2020/8
Y1 - 2020/8
N2 - HIV and EBV are human pathogens that cause a considerable burden to worldwide health. In combination, these viruses are linked to AIDS-associated lymphomas. We found that EBV, which transforms B cells, renders them susceptible to HIV-1 infection in a CXCR4 and CD4-dependent manner in vitro and that CXCR4-tropic HIV-1 integrates into the genome of these B cells with the same molecular profile as in autologous CD4+ T cells. In addition, we established a humanized mouse model to investigate the in vivo interactions of EBV and HIV-1 upon coinfection. The respective mice that reconstitute human immune system components upon transplantation with CD34+ human hematopoietic progenitor cells could recapitulate aspects of EBV and HIV immunobiology observed in dual-infected patients. Upon coinfection of humanized mice, EBV/HIV dual-infected B cells could be detected, but were susceptible to CD8+ T-cell-mediated immune control.
AB - HIV and EBV are human pathogens that cause a considerable burden to worldwide health. In combination, these viruses are linked to AIDS-associated lymphomas. We found that EBV, which transforms B cells, renders them susceptible to HIV-1 infection in a CXCR4 and CD4-dependent manner in vitro and that CXCR4-tropic HIV-1 integrates into the genome of these B cells with the same molecular profile as in autologous CD4+ T cells. In addition, we established a humanized mouse model to investigate the in vivo interactions of EBV and HIV-1 upon coinfection. The respective mice that reconstitute human immune system components upon transplantation with CD34+ human hematopoietic progenitor cells could recapitulate aspects of EBV and HIV immunobiology observed in dual-infected patients. Upon coinfection of humanized mice, EBV/HIV dual-infected B cells could be detected, but were susceptible to CD8+ T-cell-mediated immune control.
U2 - 10.26508/lsa.202000640
DO - 10.26508/lsa.202000640
M3 - SCORING: Journal article
C2 - 32576602
VL - 3
JO - LIFE SCI ALLIANCE
JF - LIFE SCI ALLIANCE
SN - 2575-1077
IS - 8
ER -