Ebola virus infection kinetics in chimeric mice reveal a key role of T cells as barriers for virus dissemination
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Ebola virus infection kinetics in chimeric mice reveal a key role of T cells as barriers for virus dissemination. / Lüdtke, Anja; Ruibal, Paula; Wozniak, David M; Pallasch, Elisa; Wurr, Stephanie; Bockholt, Sabrina; Gómez-Medina, Sergio; Qiu, Xiangguo; Kobinger, Gary P; Rodríguez, Estefanía; Günther, Stephan; Krasemann, Susanne; Idoyaga, Juliana; Oestereich, Lisa; Muñoz-Fontela, César.
In: SCI REP-UK, Vol. 7, 03.03.2017, p. 43776.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Ebola virus infection kinetics in chimeric mice reveal a key role of T cells as barriers for virus dissemination
AU - Lüdtke, Anja
AU - Ruibal, Paula
AU - Wozniak, David M
AU - Pallasch, Elisa
AU - Wurr, Stephanie
AU - Bockholt, Sabrina
AU - Gómez-Medina, Sergio
AU - Qiu, Xiangguo
AU - Kobinger, Gary P
AU - Rodríguez, Estefanía
AU - Günther, Stephan
AU - Krasemann, Susanne
AU - Idoyaga, Juliana
AU - Oestereich, Lisa
AU - Muñoz-Fontela, César
PY - 2017/3/3
Y1 - 2017/3/3
N2 - Ebola virus (EBOV) causes severe systemic disease in humans and non-human primates characterized by high levels of viremia and virus titers in peripheral organs. The natural portals of virus entry are the mucosal surfaces and the skin where macrophages and dendritic cells (DCs) are primary EBOV targets. Due to the migratory properties of DCs, EBOV infection of these cells has been proposed as a necessary step for virus dissemination via draining lymph nodes and blood. Here we utilize chimeric mice with competent hematopoietic-driven immunity, to show that EBOV primarily infects CD11b(+) DCs in non-lymphoid and lymphoid tissues, but spares the main cross-presenting CD103(+) DC subset. Furthermore, depletion of CD8 and CD4 T cells resulted in loss of early control of virus replication, viremia and fatal Ebola virus disease (EVD). Thus, our findings point out at T cell function as a key determinant of EVD progress and outcome.
AB - Ebola virus (EBOV) causes severe systemic disease in humans and non-human primates characterized by high levels of viremia and virus titers in peripheral organs. The natural portals of virus entry are the mucosal surfaces and the skin where macrophages and dendritic cells (DCs) are primary EBOV targets. Due to the migratory properties of DCs, EBOV infection of these cells has been proposed as a necessary step for virus dissemination via draining lymph nodes and blood. Here we utilize chimeric mice with competent hematopoietic-driven immunity, to show that EBOV primarily infects CD11b(+) DCs in non-lymphoid and lymphoid tissues, but spares the main cross-presenting CD103(+) DC subset. Furthermore, depletion of CD8 and CD4 T cells resulted in loss of early control of virus replication, viremia and fatal Ebola virus disease (EVD). Thus, our findings point out at T cell function as a key determinant of EVD progress and outcome.
KW - Journal Article
U2 - 10.1038/srep43776
DO - 10.1038/srep43776
M3 - SCORING: Journal article
C2 - 28256637
VL - 7
SP - 43776
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
ER -