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Dynamic expression of LIM cofactors in the developing mouse neural tube.

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Dynamic expression of LIM cofactors in the developing mouse neural tube. / Ostendorff, Heather P; Tursun, Baris; Cornils, Kerstin; Schlüter, Anne; Drung, Alexander; Güngör, Cenap; Bach, Ingolf.

In: DEV DYNAM, Vol. 235, No. 3, 3, 2006, p. 786-791.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ostendorff, HP, Tursun, B, Cornils, K, Schlüter, A, Drung, A, Güngör, C & Bach, I 2006, 'Dynamic expression of LIM cofactors in the developing mouse neural tube.', DEV DYNAM, vol. 235, no. 3, 3, pp. 786-791. <http://www.ncbi.nlm.nih.gov/pubmed/16395690?dopt=Citation>

APA

Ostendorff, H. P., Tursun, B., Cornils, K., Schlüter, A., Drung, A., Güngör, C., & Bach, I. (2006). Dynamic expression of LIM cofactors in the developing mouse neural tube. DEV DYNAM, 235(3), 786-791. [3]. http://www.ncbi.nlm.nih.gov/pubmed/16395690?dopt=Citation

Vancouver

Ostendorff HP, Tursun B, Cornils K, Schlüter A, Drung A, Güngör C et al. Dynamic expression of LIM cofactors in the developing mouse neural tube. DEV DYNAM. 2006;235(3):786-791. 3.

Bibtex

@article{6e501645ad8b4724a46b50b08d9c275a,
title = "Dynamic expression of LIM cofactors in the developing mouse neural tube.",
abstract = "The developmental regulation of LIM homeodomain transcription factors (LIM-HD) by the LIM domain-binding cofactors CLIM/Ldb/NLI and RLIM has been demonstrated. Whereas CLIM cofactors are thought to be required for at least some of the in vivo functions of LIM-HD proteins, the ubiquitin ligase RLIM functions as a negative regulator by its ability to target CLIM cofactors for proteasomal degradation. In this report, we have investigated and compared the protein expression of both factors in the developing mouse neural tube. We co-localize both proteins in many tissues and, although widely expressed, we detect high levels of both cofactors in specific neural tube regions, e.g., in the ventral neural tube, where motor neurons reside. The mostly ubiquitous distribution of RLIM- and CLIM-encoding mRNA differs from the more specific expression of both cofactors at the protein level, indicating post-transcriptional regulation. Furthermore, we show that both cofactors not only co-localize with each other but also with Isl and Lhx3 LIM-HD proteins in developing ventral neural tube neurons. Our results demonstrate the dynamic expression of cofactors participating in the regulation of LIM-HD proteins during the development of the neural tube in mice and suggest additional post-transcriptional regulation in the nuclear LIM-HD protein network.",
keywords = "Animals, Central Nervous System/chemistry/*embryology, DNA-Binding Proteins/analysis/genetics/*metabolism, Homeodomain Proteins/analysis/genetics/*metabolism, LIM Domain Proteins, LIM-Homeodomain Proteins, Metalloproteins/analysis, Mice/*embryology/genetics/metabolism, RNA, Messenger/analysis/metabolism, Repressor Proteins/analysis/genetics/*metabolism, Transcription Factors/analysis/genetics/*metabolism, Ubiquitin-Protein Ligases, Animals, Central Nervous System/chemistry/*embryology, DNA-Binding Proteins/analysis/genetics/*metabolism, Homeodomain Proteins/analysis/genetics/*metabolism, LIM Domain Proteins, LIM-Homeodomain Proteins, Metalloproteins/analysis, Mice/*embryology/genetics/metabolism, RNA, Messenger/analysis/metabolism, Repressor Proteins/analysis/genetics/*metabolism, Transcription Factors/analysis/genetics/*metabolism, Ubiquitin-Protein Ligases",
author = "Ostendorff, {Heather P} and Baris Tursun and Kerstin Cornils and Anne Schl{\"u}ter and Alexander Drung and Cenap G{\"u}ng{\"o}r and Ingolf Bach",
year = "2006",
language = "English",
volume = "235",
pages = "786--791",
journal = "DEV DYNAM",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Dynamic expression of LIM cofactors in the developing mouse neural tube.

AU - Ostendorff, Heather P

AU - Tursun, Baris

AU - Cornils, Kerstin

AU - Schlüter, Anne

AU - Drung, Alexander

AU - Güngör, Cenap

AU - Bach, Ingolf

PY - 2006

Y1 - 2006

N2 - The developmental regulation of LIM homeodomain transcription factors (LIM-HD) by the LIM domain-binding cofactors CLIM/Ldb/NLI and RLIM has been demonstrated. Whereas CLIM cofactors are thought to be required for at least some of the in vivo functions of LIM-HD proteins, the ubiquitin ligase RLIM functions as a negative regulator by its ability to target CLIM cofactors for proteasomal degradation. In this report, we have investigated and compared the protein expression of both factors in the developing mouse neural tube. We co-localize both proteins in many tissues and, although widely expressed, we detect high levels of both cofactors in specific neural tube regions, e.g., in the ventral neural tube, where motor neurons reside. The mostly ubiquitous distribution of RLIM- and CLIM-encoding mRNA differs from the more specific expression of both cofactors at the protein level, indicating post-transcriptional regulation. Furthermore, we show that both cofactors not only co-localize with each other but also with Isl and Lhx3 LIM-HD proteins in developing ventral neural tube neurons. Our results demonstrate the dynamic expression of cofactors participating in the regulation of LIM-HD proteins during the development of the neural tube in mice and suggest additional post-transcriptional regulation in the nuclear LIM-HD protein network.

AB - The developmental regulation of LIM homeodomain transcription factors (LIM-HD) by the LIM domain-binding cofactors CLIM/Ldb/NLI and RLIM has been demonstrated. Whereas CLIM cofactors are thought to be required for at least some of the in vivo functions of LIM-HD proteins, the ubiquitin ligase RLIM functions as a negative regulator by its ability to target CLIM cofactors for proteasomal degradation. In this report, we have investigated and compared the protein expression of both factors in the developing mouse neural tube. We co-localize both proteins in many tissues and, although widely expressed, we detect high levels of both cofactors in specific neural tube regions, e.g., in the ventral neural tube, where motor neurons reside. The mostly ubiquitous distribution of RLIM- and CLIM-encoding mRNA differs from the more specific expression of both cofactors at the protein level, indicating post-transcriptional regulation. Furthermore, we show that both cofactors not only co-localize with each other but also with Isl and Lhx3 LIM-HD proteins in developing ventral neural tube neurons. Our results demonstrate the dynamic expression of cofactors participating in the regulation of LIM-HD proteins during the development of the neural tube in mice and suggest additional post-transcriptional regulation in the nuclear LIM-HD protein network.

KW - Animals

KW - Central Nervous System/chemistry/embryology

KW - DNA-Binding Proteins/analysis/genetics/metabolism

KW - Homeodomain Proteins/analysis/genetics/metabolism

KW - LIM Domain Proteins

KW - LIM-Homeodomain Proteins

KW - Metalloproteins/analysis

KW - Mice/embryology/genetics/metabolism

KW - RNA, Messenger/analysis/metabolism

KW - Repressor Proteins/analysis/genetics/metabolism

KW - Transcription Factors/analysis/genetics/metabolism

KW - Ubiquitin-Protein Ligases

KW - Animals

KW - Central Nervous System/chemistry/embryology

KW - DNA-Binding Proteins/analysis/genetics/metabolism

KW - Homeodomain Proteins/analysis/genetics/metabolism

KW - LIM Domain Proteins

KW - LIM-Homeodomain Proteins

KW - Metalloproteins/analysis

KW - Mice/embryology/genetics/metabolism

KW - RNA, Messenger/analysis/metabolism

KW - Repressor Proteins/analysis/genetics/metabolism

KW - Transcription Factors/analysis/genetics/metabolism

KW - Ubiquitin-Protein Ligases

M3 - SCORING: Journal article

VL - 235

SP - 786

EP - 791

JO - DEV DYNAM

JF - DEV DYNAM

SN - 1058-8388

IS - 3

M1 - 3

ER -