Dynamic expression of LIM cofactors in the developing mouse neural tube.
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Dynamic expression of LIM cofactors in the developing mouse neural tube. / Ostendorff, Heather P; Tursun, Baris; Cornils, Kerstin; Schlüter, Anne; Drung, Alexander; Güngör, Cenap; Bach, Ingolf.
in: DEV DYNAM, Jahrgang 235, Nr. 3, 3, 2006, S. 786-791.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Dynamic expression of LIM cofactors in the developing mouse neural tube.
AU - Ostendorff, Heather P
AU - Tursun, Baris
AU - Cornils, Kerstin
AU - Schlüter, Anne
AU - Drung, Alexander
AU - Güngör, Cenap
AU - Bach, Ingolf
PY - 2006
Y1 - 2006
N2 - The developmental regulation of LIM homeodomain transcription factors (LIM-HD) by the LIM domain-binding cofactors CLIM/Ldb/NLI and RLIM has been demonstrated. Whereas CLIM cofactors are thought to be required for at least some of the in vivo functions of LIM-HD proteins, the ubiquitin ligase RLIM functions as a negative regulator by its ability to target CLIM cofactors for proteasomal degradation. In this report, we have investigated and compared the protein expression of both factors in the developing mouse neural tube. We co-localize both proteins in many tissues and, although widely expressed, we detect high levels of both cofactors in specific neural tube regions, e.g., in the ventral neural tube, where motor neurons reside. The mostly ubiquitous distribution of RLIM- and CLIM-encoding mRNA differs from the more specific expression of both cofactors at the protein level, indicating post-transcriptional regulation. Furthermore, we show that both cofactors not only co-localize with each other but also with Isl and Lhx3 LIM-HD proteins in developing ventral neural tube neurons. Our results demonstrate the dynamic expression of cofactors participating in the regulation of LIM-HD proteins during the development of the neural tube in mice and suggest additional post-transcriptional regulation in the nuclear LIM-HD protein network.
AB - The developmental regulation of LIM homeodomain transcription factors (LIM-HD) by the LIM domain-binding cofactors CLIM/Ldb/NLI and RLIM has been demonstrated. Whereas CLIM cofactors are thought to be required for at least some of the in vivo functions of LIM-HD proteins, the ubiquitin ligase RLIM functions as a negative regulator by its ability to target CLIM cofactors for proteasomal degradation. In this report, we have investigated and compared the protein expression of both factors in the developing mouse neural tube. We co-localize both proteins in many tissues and, although widely expressed, we detect high levels of both cofactors in specific neural tube regions, e.g., in the ventral neural tube, where motor neurons reside. The mostly ubiquitous distribution of RLIM- and CLIM-encoding mRNA differs from the more specific expression of both cofactors at the protein level, indicating post-transcriptional regulation. Furthermore, we show that both cofactors not only co-localize with each other but also with Isl and Lhx3 LIM-HD proteins in developing ventral neural tube neurons. Our results demonstrate the dynamic expression of cofactors participating in the regulation of LIM-HD proteins during the development of the neural tube in mice and suggest additional post-transcriptional regulation in the nuclear LIM-HD protein network.
KW - Animals
KW - Central Nervous System/chemistry/embryology
KW - DNA-Binding Proteins/analysis/genetics/metabolism
KW - Homeodomain Proteins/analysis/genetics/metabolism
KW - LIM Domain Proteins
KW - LIM-Homeodomain Proteins
KW - Metalloproteins/analysis
KW - Mice/embryology/genetics/metabolism
KW - RNA, Messenger/analysis/metabolism
KW - Repressor Proteins/analysis/genetics/metabolism
KW - Transcription Factors/analysis/genetics/metabolism
KW - Ubiquitin-Protein Ligases
KW - Animals
KW - Central Nervous System/chemistry/embryology
KW - DNA-Binding Proteins/analysis/genetics/metabolism
KW - Homeodomain Proteins/analysis/genetics/metabolism
KW - LIM Domain Proteins
KW - LIM-Homeodomain Proteins
KW - Metalloproteins/analysis
KW - Mice/embryology/genetics/metabolism
KW - RNA, Messenger/analysis/metabolism
KW - Repressor Proteins/analysis/genetics/metabolism
KW - Transcription Factors/analysis/genetics/metabolism
KW - Ubiquitin-Protein Ligases
M3 - SCORING: Journal article
VL - 235
SP - 786
EP - 791
JO - DEV DYNAM
JF - DEV DYNAM
SN - 1058-8388
IS - 3
M1 - 3
ER -