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DNA binding, protein interaction and differential expression of the human germ cell nuclear factor

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DNA binding, protein interaction and differential expression of the human germ cell nuclear factor. / Schmitz, T P; Süsens, U; Borgmeyer, U.

In: Biochim Biophys Acta, Vol. 1446, No. 3, 03.09.1999, p. 173-80.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schmitz, TP, Süsens, U & Borgmeyer, U 1999, 'DNA binding, protein interaction and differential expression of the human germ cell nuclear factor', Biochim Biophys Acta, vol. 1446, no. 3, pp. 173-80.

APA

Schmitz, T. P., Süsens, U., & Borgmeyer, U. (1999). DNA binding, protein interaction and differential expression of the human germ cell nuclear factor. Biochim Biophys Acta, 1446(3), 173-80.

Vancouver

Schmitz TP, Süsens U, Borgmeyer U. DNA binding, protein interaction and differential expression of the human germ cell nuclear factor. Biochim Biophys Acta. 1999 Sep 3;1446(3):173-80.

Bibtex

@article{ef67ddbd69f24f09a2015e33ee66c964,
title = "DNA binding, protein interaction and differential expression of the human germ cell nuclear factor",
abstract = "The mouse germ cell nuclear factor (mGCNF) is an orphan nuclear receptor implicated in diverse biological processes, including gametogenesis, embryonic development and embryonal carcinoma cell differentiation. We have examined the binding and regulation of the human orthologue, hGCNF, expressed in the teratocarcinoma-derived cell line NTera-2/clone D1 (NT2/D1). Binding of GCNF to the direct repeat of the sequence -AGGTCA- (DR-0) is conserved in mammalia. The formation of interspecies dimers of the in vitro synthesized proteins suggests that cellular GCNF binding is mediated by homodimers. Both the mouse and the human protein bind in concert with cellular factors to DNA. Treatment of NT2/D1 cells with all-trans retinoic acid (atRA) is accompanied first by an up-regulation followed later by a down-regulation of hGCNF and its mRNA. Temporary up-regulation in NT2/D1 cells after treatment with atRA suggests that hGCNF is important for human neural determination and differentiation.",
keywords = "Animals, COS Cells, Cell Extracts, DNA, DNA-Binding Proteins, Dimerization, Gene Expression Regulation, Humans, Nuclear Receptor Subfamily 6, Group A, Member 1, RNA, Messenger, Receptors, Cytoplasmic and Nuclear, Recombinant Proteins, Tretinoin, Tumor Cells, Cultured",
author = "Schmitz, {T P} and U S{\"u}sens and U Borgmeyer",
year = "1999",
month = sep,
day = "3",
language = "English",
volume = "1446",
pages = "173--80",
journal = "Biochim Biophys Acta",
issn = "0006-3002",
number = "3",

}

RIS

TY - JOUR

T1 - DNA binding, protein interaction and differential expression of the human germ cell nuclear factor

AU - Schmitz, T P

AU - Süsens, U

AU - Borgmeyer, U

PY - 1999/9/3

Y1 - 1999/9/3

N2 - The mouse germ cell nuclear factor (mGCNF) is an orphan nuclear receptor implicated in diverse biological processes, including gametogenesis, embryonic development and embryonal carcinoma cell differentiation. We have examined the binding and regulation of the human orthologue, hGCNF, expressed in the teratocarcinoma-derived cell line NTera-2/clone D1 (NT2/D1). Binding of GCNF to the direct repeat of the sequence -AGGTCA- (DR-0) is conserved in mammalia. The formation of interspecies dimers of the in vitro synthesized proteins suggests that cellular GCNF binding is mediated by homodimers. Both the mouse and the human protein bind in concert with cellular factors to DNA. Treatment of NT2/D1 cells with all-trans retinoic acid (atRA) is accompanied first by an up-regulation followed later by a down-regulation of hGCNF and its mRNA. Temporary up-regulation in NT2/D1 cells after treatment with atRA suggests that hGCNF is important for human neural determination and differentiation.

AB - The mouse germ cell nuclear factor (mGCNF) is an orphan nuclear receptor implicated in diverse biological processes, including gametogenesis, embryonic development and embryonal carcinoma cell differentiation. We have examined the binding and regulation of the human orthologue, hGCNF, expressed in the teratocarcinoma-derived cell line NTera-2/clone D1 (NT2/D1). Binding of GCNF to the direct repeat of the sequence -AGGTCA- (DR-0) is conserved in mammalia. The formation of interspecies dimers of the in vitro synthesized proteins suggests that cellular GCNF binding is mediated by homodimers. Both the mouse and the human protein bind in concert with cellular factors to DNA. Treatment of NT2/D1 cells with all-trans retinoic acid (atRA) is accompanied first by an up-regulation followed later by a down-regulation of hGCNF and its mRNA. Temporary up-regulation in NT2/D1 cells after treatment with atRA suggests that hGCNF is important for human neural determination and differentiation.

KW - Animals

KW - COS Cells

KW - Cell Extracts

KW - DNA

KW - DNA-Binding Proteins

KW - Dimerization

KW - Gene Expression Regulation

KW - Humans

KW - Nuclear Receptor Subfamily 6, Group A, Member 1

KW - RNA, Messenger

KW - Receptors, Cytoplasmic and Nuclear

KW - Recombinant Proteins

KW - Tretinoin

KW - Tumor Cells, Cultured

M3 - SCORING: Journal article

C2 - 10524192

VL - 1446

SP - 173

EP - 180

JO - Biochim Biophys Acta

JF - Biochim Biophys Acta

SN - 0006-3002

IS - 3

ER -