DNA binding, protein interaction and differential expression of the human germ cell nuclear factor
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DNA binding, protein interaction and differential expression of the human germ cell nuclear factor. / Schmitz, T P; Süsens, U; Borgmeyer, U.
in: Biochim Biophys Acta, Jahrgang 1446, Nr. 3, 03.09.1999, S. 173-80.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - DNA binding, protein interaction and differential expression of the human germ cell nuclear factor
AU - Schmitz, T P
AU - Süsens, U
AU - Borgmeyer, U
PY - 1999/9/3
Y1 - 1999/9/3
N2 - The mouse germ cell nuclear factor (mGCNF) is an orphan nuclear receptor implicated in diverse biological processes, including gametogenesis, embryonic development and embryonal carcinoma cell differentiation. We have examined the binding and regulation of the human orthologue, hGCNF, expressed in the teratocarcinoma-derived cell line NTera-2/clone D1 (NT2/D1). Binding of GCNF to the direct repeat of the sequence -AGGTCA- (DR-0) is conserved in mammalia. The formation of interspecies dimers of the in vitro synthesized proteins suggests that cellular GCNF binding is mediated by homodimers. Both the mouse and the human protein bind in concert with cellular factors to DNA. Treatment of NT2/D1 cells with all-trans retinoic acid (atRA) is accompanied first by an up-regulation followed later by a down-regulation of hGCNF and its mRNA. Temporary up-regulation in NT2/D1 cells after treatment with atRA suggests that hGCNF is important for human neural determination and differentiation.
AB - The mouse germ cell nuclear factor (mGCNF) is an orphan nuclear receptor implicated in diverse biological processes, including gametogenesis, embryonic development and embryonal carcinoma cell differentiation. We have examined the binding and regulation of the human orthologue, hGCNF, expressed in the teratocarcinoma-derived cell line NTera-2/clone D1 (NT2/D1). Binding of GCNF to the direct repeat of the sequence -AGGTCA- (DR-0) is conserved in mammalia. The formation of interspecies dimers of the in vitro synthesized proteins suggests that cellular GCNF binding is mediated by homodimers. Both the mouse and the human protein bind in concert with cellular factors to DNA. Treatment of NT2/D1 cells with all-trans retinoic acid (atRA) is accompanied first by an up-regulation followed later by a down-regulation of hGCNF and its mRNA. Temporary up-regulation in NT2/D1 cells after treatment with atRA suggests that hGCNF is important for human neural determination and differentiation.
KW - Animals
KW - COS Cells
KW - Cell Extracts
KW - DNA
KW - DNA-Binding Proteins
KW - Dimerization
KW - Gene Expression Regulation
KW - Humans
KW - Nuclear Receptor Subfamily 6, Group A, Member 1
KW - RNA, Messenger
KW - Receptors, Cytoplasmic and Nuclear
KW - Recombinant Proteins
KW - Tretinoin
KW - Tumor Cells, Cultured
M3 - SCORING: Journal article
C2 - 10524192
VL - 1446
SP - 173
EP - 180
JO - Biochim Biophys Acta
JF - Biochim Biophys Acta
SN - 0006-3002
IS - 3
ER -