Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry
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TY - JOUR
T1 - Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry
AU - Dubben, H H
PY - 1990/3/1
Y1 - 1990/3/1
N2 - The aim of this study was to investigate the use of a nucleolar antigen to discriminate between proliferating and resting cells. Antinucleolar antibodies (Si87) were obtained from a scleroderma patient. The specificity of immunostaining was verified and morphological changes in nucleoli were monitored using a fluorescence microscope. Fluorescence of propidium iodide-stained DNA and nucleolar immunofluorescence were measured by flow cytometry. Following phytohaemagglutinin stimulation the number of nucleoli of normal human peripheral blood lymphocytes increased about 3-fold, accompanied by enlargement of nucleolar size. Simultaneously a mean increase in total immunofluorescence per cell by a factor of three was detected. The method developed and applied here allows a discrimination between resting and proliferating human lymphocytes on the basis of their nucleolar antigen content.
AB - The aim of this study was to investigate the use of a nucleolar antigen to discriminate between proliferating and resting cells. Antinucleolar antibodies (Si87) were obtained from a scleroderma patient. The specificity of immunostaining was verified and morphological changes in nucleoli were monitored using a fluorescence microscope. Fluorescence of propidium iodide-stained DNA and nucleolar immunofluorescence were measured by flow cytometry. Following phytohaemagglutinin stimulation the number of nucleoli of normal human peripheral blood lymphocytes increased about 3-fold, accompanied by enlargement of nucleolar size. Simultaneously a mean increase in total immunofluorescence per cell by a factor of three was detected. The method developed and applied here allows a discrimination between resting and proliferating human lymphocytes on the basis of their nucleolar antigen content.
KW - Animals
KW - Antibodies
KW - Antibodies, Antinuclear
KW - Antigens
KW - Cell Division
KW - Cell Nucleolus
KW - Cells, Cultured
KW - Deoxyribonucleases
KW - Flow Cytometry
KW - Fluorescent Antibody Technique
KW - Humans
KW - Lymphocytes
KW - Mice
KW - Microscopy, Fluorescence
KW - Phytohemagglutinins
KW - Ribonucleases
KW - Scleroderma, Systemic
M3 - SCORING: Journal article
C2 - 2180573
VL - 23
SP - 89
EP - 97
IS - 2
ER -