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Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry

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Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry. / Dubben, H H.

in: Cell Tissue Kinet, Jahrgang 23, Nr. 2, 01.03.1990, S. 89-97.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Dubben, HH 1990, 'Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry', Cell Tissue Kinet, Jg. 23, Nr. 2, S. 89-97.

APA

Dubben, H. H. (1990). Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry. Cell Tissue Kinet, 23(2), 89-97.

Vancouver

Dubben HH. Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry. Cell Tissue Kinet. 1990 Mär 1;23(2):89-97.

Bibtex

@article{a0ce56a2bcf94e4391d00bbc13635220,
title = "Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry",
abstract = "The aim of this study was to investigate the use of a nucleolar antigen to discriminate between proliferating and resting cells. Antinucleolar antibodies (Si87) were obtained from a scleroderma patient. The specificity of immunostaining was verified and morphological changes in nucleoli were monitored using a fluorescence microscope. Fluorescence of propidium iodide-stained DNA and nucleolar immunofluorescence were measured by flow cytometry. Following phytohaemagglutinin stimulation the number of nucleoli of normal human peripheral blood lymphocytes increased about 3-fold, accompanied by enlargement of nucleolar size. Simultaneously a mean increase in total immunofluorescence per cell by a factor of three was detected. The method developed and applied here allows a discrimination between resting and proliferating human lymphocytes on the basis of their nucleolar antigen content.",
keywords = "Animals, Antibodies, Antibodies, Antinuclear, Antigens, Cell Division, Cell Nucleolus, Cells, Cultured, Deoxyribonucleases, Flow Cytometry, Fluorescent Antibody Technique, Humans, Lymphocytes, Mice, Microscopy, Fluorescence, Phytohemagglutinins, Ribonucleases, Scleroderma, Systemic",
author = "Dubben, {H H}",
year = "1990",
month = mar,
day = "1",
language = "English",
volume = "23",
pages = "89--97",
number = "2",

}

RIS

TY - JOUR

T1 - Different nucleolar antigen expression in resting and proliferating human lymphocytes as studied by fluorescence microscopy and flow cytometry

AU - Dubben, H H

PY - 1990/3/1

Y1 - 1990/3/1

N2 - The aim of this study was to investigate the use of a nucleolar antigen to discriminate between proliferating and resting cells. Antinucleolar antibodies (Si87) were obtained from a scleroderma patient. The specificity of immunostaining was verified and morphological changes in nucleoli were monitored using a fluorescence microscope. Fluorescence of propidium iodide-stained DNA and nucleolar immunofluorescence were measured by flow cytometry. Following phytohaemagglutinin stimulation the number of nucleoli of normal human peripheral blood lymphocytes increased about 3-fold, accompanied by enlargement of nucleolar size. Simultaneously a mean increase in total immunofluorescence per cell by a factor of three was detected. The method developed and applied here allows a discrimination between resting and proliferating human lymphocytes on the basis of their nucleolar antigen content.

AB - The aim of this study was to investigate the use of a nucleolar antigen to discriminate between proliferating and resting cells. Antinucleolar antibodies (Si87) were obtained from a scleroderma patient. The specificity of immunostaining was verified and morphological changes in nucleoli were monitored using a fluorescence microscope. Fluorescence of propidium iodide-stained DNA and nucleolar immunofluorescence were measured by flow cytometry. Following phytohaemagglutinin stimulation the number of nucleoli of normal human peripheral blood lymphocytes increased about 3-fold, accompanied by enlargement of nucleolar size. Simultaneously a mean increase in total immunofluorescence per cell by a factor of three was detected. The method developed and applied here allows a discrimination between resting and proliferating human lymphocytes on the basis of their nucleolar antigen content.

KW - Animals

KW - Antibodies

KW - Antibodies, Antinuclear

KW - Antigens

KW - Cell Division

KW - Cell Nucleolus

KW - Cells, Cultured

KW - Deoxyribonucleases

KW - Flow Cytometry

KW - Fluorescent Antibody Technique

KW - Humans

KW - Lymphocytes

KW - Mice

KW - Microscopy, Fluorescence

KW - Phytohemagglutinins

KW - Ribonucleases

KW - Scleroderma, Systemic

M3 - SCORING: Journal article

C2 - 2180573

VL - 23

SP - 89

EP - 97

IS - 2

ER -