Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso
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Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso. / Al-Emran, Hassan M; Hahn, Andreas; Baum, Jana; Cruz Espinoza, Ligia Maria; Deerin, Jessica; Im, Justin; Ibrango, Samuel; Kabore, Leon Parfait; von Kalckreuth, Vera; Konings, Frank; Marks, Florian; Sampo, Emmanuel; Panzner, Ursula; Park, Se Eun; Pak, Gi Deok; Schütt-Gerowitt, Heidi; Vinnemeier, Christof David; Warren, Michelle; Soura, Abdramane Bassiahi.
In: CLIN INFECT DIS, Vol. 62 , No. Suppl 1, 15.03.2016, p. S37-41.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso
AU - Al-Emran, Hassan M
AU - Hahn, Andreas
AU - Baum, Jana
AU - Cruz Espinoza, Ligia Maria
AU - Deerin, Jessica
AU - Im, Justin
AU - Ibrango, Samuel
AU - Kabore, Leon Parfait
AU - von Kalckreuth, Vera
AU - Konings, Frank
AU - Marks, Florian
AU - Sampo, Emmanuel
AU - Panzner, Ursula
AU - Park, Se Eun
AU - Pak, Gi Deok
AU - Schütt-Gerowitt, Heidi
AU - Vinnemeier, Christof David
AU - Warren, Michelle
AU - Soura, Abdramane Bassiahi
N1 - © The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
PY - 2016/3/15
Y1 - 2016/3/15
N2 - BACKGROUND: Globally, there are an estimated 22 million cases of Salmonella enterica serovar Typhi infection each year. However, this figure is likely to be an underestimate due to the low sensitivity of blood culture in S. Typhi diagnosis. The aim of this study was to diagnose S. Typhi by conventional polymerase chain reaction (PCR) using patient's blood preserved with ethylenediamine tetraacetic acid (EDTA).METHODS: From April 2012 to September 2013, typhoid fever surveillance was conducted in Polesgo and Nioko, 2 dry slum areas in Ouagadougou, Burkina Faso. Blood culture was performed for febrile patients using an automated blood culture system. Additional blood was collected in EDTA tubes from those patients and preserved at -80°C. DNA was extracted from EDTA blood and PCR was performed to identify presence of S. Typhi. Randomly selected PCR products were further sequenced to identify S. Typhi-specific amplicons.RESULTS: Of 1674 patients, S. Typhi was isolated from 18 (1.1%) individuals by blood culture. EDTA blood was collected from 1578 patients, of which 298 EDTA samples were tested by PCR. Salmonella Typhi-specific DNA was identified in 44 (14.8%) samples. The sensitivity of S. Typhi-specific PCR from EDTA blood was 89% (74%-100%) among the blood culture-positive cases. Sixteen S. Typhi-positive PCR products were sequenced, and 13 retrieved the sequence of a S. Typhi-specific amplicon.CONCLUSIONS: These findings suggest that blood culture-based diagnoses of S. Typhi underestimate the burden of typhoid fever in Burkina Faso. PCR could be considered as an alternative method for the identification and diagnosis of S. Typhi in blood samples.
AB - BACKGROUND: Globally, there are an estimated 22 million cases of Salmonella enterica serovar Typhi infection each year. However, this figure is likely to be an underestimate due to the low sensitivity of blood culture in S. Typhi diagnosis. The aim of this study was to diagnose S. Typhi by conventional polymerase chain reaction (PCR) using patient's blood preserved with ethylenediamine tetraacetic acid (EDTA).METHODS: From April 2012 to September 2013, typhoid fever surveillance was conducted in Polesgo and Nioko, 2 dry slum areas in Ouagadougou, Burkina Faso. Blood culture was performed for febrile patients using an automated blood culture system. Additional blood was collected in EDTA tubes from those patients and preserved at -80°C. DNA was extracted from EDTA blood and PCR was performed to identify presence of S. Typhi. Randomly selected PCR products were further sequenced to identify S. Typhi-specific amplicons.RESULTS: Of 1674 patients, S. Typhi was isolated from 18 (1.1%) individuals by blood culture. EDTA blood was collected from 1578 patients, of which 298 EDTA samples were tested by PCR. Salmonella Typhi-specific DNA was identified in 44 (14.8%) samples. The sensitivity of S. Typhi-specific PCR from EDTA blood was 89% (74%-100%) among the blood culture-positive cases. Sixteen S. Typhi-positive PCR products were sequenced, and 13 retrieved the sequence of a S. Typhi-specific amplicon.CONCLUSIONS: These findings suggest that blood culture-based diagnoses of S. Typhi underestimate the burden of typhoid fever in Burkina Faso. PCR could be considered as an alternative method for the identification and diagnosis of S. Typhi in blood samples.
KW - Adolescent
KW - Adult
KW - Bacteremia
KW - Burkina Faso
KW - Child
KW - Child, Preschool
KW - Cohort Studies
KW - DNA, Bacterial
KW - Edetic Acid
KW - Female
KW - Fever
KW - Humans
KW - Infant
KW - Infant, Newborn
KW - Male
KW - Molecular Typing
KW - Polymerase Chain Reaction
KW - Public Health Surveillance
KW - Salmonella typhi
KW - Typhoid Fever
KW - Young Adult
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1093/cid/civ770
DO - 10.1093/cid/civ770
M3 - SCORING: Journal article
C2 - 26933018
VL - 62
SP - S37-41
JO - CLIN INFECT DIS
JF - CLIN INFECT DIS
SN - 1058-4838
IS - Suppl 1
ER -