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Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso

Standard

Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso. / Al-Emran, Hassan M; Hahn, Andreas; Baum, Jana; Cruz Espinoza, Ligia Maria; Deerin, Jessica; Im, Justin; Ibrango, Samuel; Kabore, Leon Parfait; von Kalckreuth, Vera; Konings, Frank; Marks, Florian; Sampo, Emmanuel; Panzner, Ursula; Park, Se Eun; Pak, Gi Deok; Schütt-Gerowitt, Heidi; Vinnemeier, Christof David; Warren, Michelle; Soura, Abdramane Bassiahi.

in: CLIN INFECT DIS, Jahrgang 62 , Nr. Suppl 1, 15.03.2016, S. S37-41.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Al-Emran, HM, Hahn, A, Baum, J, Cruz Espinoza, LM, Deerin, J, Im, J, Ibrango, S, Kabore, LP, von Kalckreuth, V, Konings, F, Marks, F, Sampo, E, Panzner, U, Park, SE, Pak, GD, Schütt-Gerowitt, H, Vinnemeier, CD, Warren, M & Soura, AB 2016, 'Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso', CLIN INFECT DIS, Jg. 62 , Nr. Suppl 1, S. S37-41. https://doi.org/10.1093/cid/civ770

APA

Al-Emran, H. M., Hahn, A., Baum, J., Cruz Espinoza, L. M., Deerin, J., Im, J., Ibrango, S., Kabore, L. P., von Kalckreuth, V., Konings, F., Marks, F., Sampo, E., Panzner, U., Park, S. E., Pak, G. D., Schütt-Gerowitt, H., Vinnemeier, C. D., Warren, M., & Soura, A. B. (2016). Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso. CLIN INFECT DIS, 62 (Suppl 1), S37-41. https://doi.org/10.1093/cid/civ770

Vancouver

Al-Emran HM, Hahn A, Baum J, Cruz Espinoza LM, Deerin J, Im J et al. Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso. CLIN INFECT DIS. 2016 Mär 15;62 (Suppl 1):S37-41. https://doi.org/10.1093/cid/civ770

Bibtex

@article{90c46eec43c241e5892182f489a88ed7,
title = "Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso",
abstract = "BACKGROUND: Globally, there are an estimated 22 million cases of Salmonella enterica serovar Typhi infection each year. However, this figure is likely to be an underestimate due to the low sensitivity of blood culture in S. Typhi diagnosis. The aim of this study was to diagnose S. Typhi by conventional polymerase chain reaction (PCR) using patient's blood preserved with ethylenediamine tetraacetic acid (EDTA).METHODS: From April 2012 to September 2013, typhoid fever surveillance was conducted in Polesgo and Nioko, 2 dry slum areas in Ouagadougou, Burkina Faso. Blood culture was performed for febrile patients using an automated blood culture system. Additional blood was collected in EDTA tubes from those patients and preserved at -80°C. DNA was extracted from EDTA blood and PCR was performed to identify presence of S. Typhi. Randomly selected PCR products were further sequenced to identify S. Typhi-specific amplicons.RESULTS: Of 1674 patients, S. Typhi was isolated from 18 (1.1%) individuals by blood culture. EDTA blood was collected from 1578 patients, of which 298 EDTA samples were tested by PCR. Salmonella Typhi-specific DNA was identified in 44 (14.8%) samples. The sensitivity of S. Typhi-specific PCR from EDTA blood was 89% (74%-100%) among the blood culture-positive cases. Sixteen S. Typhi-positive PCR products were sequenced, and 13 retrieved the sequence of a S. Typhi-specific amplicon.CONCLUSIONS: These findings suggest that blood culture-based diagnoses of S. Typhi underestimate the burden of typhoid fever in Burkina Faso. PCR could be considered as an alternative method for the identification and diagnosis of S. Typhi in blood samples.",
keywords = "Adolescent, Adult, Bacteremia, Burkina Faso, Child, Child, Preschool, Cohort Studies, DNA, Bacterial, Edetic Acid, Female, Fever, Humans, Infant, Infant, Newborn, Male, Molecular Typing, Polymerase Chain Reaction, Public Health Surveillance, Salmonella typhi, Typhoid Fever, Young Adult, Journal Article, Research Support, Non-U.S. Gov't",
author = "Al-Emran, {Hassan M} and Andreas Hahn and Jana Baum and {Cruz Espinoza}, {Ligia Maria} and Jessica Deerin and Justin Im and Samuel Ibrango and Kabore, {Leon Parfait} and {von Kalckreuth}, Vera and Frank Konings and Florian Marks and Emmanuel Sampo and Ursula Panzner and Park, {Se Eun} and Pak, {Gi Deok} and Heidi Sch{\"u}tt-Gerowitt and Vinnemeier, {Christof David} and Michelle Warren and Soura, {Abdramane Bassiahi}",
note = "{\textcopyright} The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.",
year = "2016",
month = mar,
day = "15",
doi = "10.1093/cid/civ770",
language = "English",
volume = "62 ",
pages = "S37--41",
journal = "CLIN INFECT DIS",
issn = "1058-4838",
publisher = "Oxford University Press",
number = "Suppl 1",

}

RIS

TY - JOUR

T1 - Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso

AU - Al-Emran, Hassan M

AU - Hahn, Andreas

AU - Baum, Jana

AU - Cruz Espinoza, Ligia Maria

AU - Deerin, Jessica

AU - Im, Justin

AU - Ibrango, Samuel

AU - Kabore, Leon Parfait

AU - von Kalckreuth, Vera

AU - Konings, Frank

AU - Marks, Florian

AU - Sampo, Emmanuel

AU - Panzner, Ursula

AU - Park, Se Eun

AU - Pak, Gi Deok

AU - Schütt-Gerowitt, Heidi

AU - Vinnemeier, Christof David

AU - Warren, Michelle

AU - Soura, Abdramane Bassiahi

N1 - © The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

PY - 2016/3/15

Y1 - 2016/3/15

N2 - BACKGROUND: Globally, there are an estimated 22 million cases of Salmonella enterica serovar Typhi infection each year. However, this figure is likely to be an underestimate due to the low sensitivity of blood culture in S. Typhi diagnosis. The aim of this study was to diagnose S. Typhi by conventional polymerase chain reaction (PCR) using patient's blood preserved with ethylenediamine tetraacetic acid (EDTA).METHODS: From April 2012 to September 2013, typhoid fever surveillance was conducted in Polesgo and Nioko, 2 dry slum areas in Ouagadougou, Burkina Faso. Blood culture was performed for febrile patients using an automated blood culture system. Additional blood was collected in EDTA tubes from those patients and preserved at -80°C. DNA was extracted from EDTA blood and PCR was performed to identify presence of S. Typhi. Randomly selected PCR products were further sequenced to identify S. Typhi-specific amplicons.RESULTS: Of 1674 patients, S. Typhi was isolated from 18 (1.1%) individuals by blood culture. EDTA blood was collected from 1578 patients, of which 298 EDTA samples were tested by PCR. Salmonella Typhi-specific DNA was identified in 44 (14.8%) samples. The sensitivity of S. Typhi-specific PCR from EDTA blood was 89% (74%-100%) among the blood culture-positive cases. Sixteen S. Typhi-positive PCR products were sequenced, and 13 retrieved the sequence of a S. Typhi-specific amplicon.CONCLUSIONS: These findings suggest that blood culture-based diagnoses of S. Typhi underestimate the burden of typhoid fever in Burkina Faso. PCR could be considered as an alternative method for the identification and diagnosis of S. Typhi in blood samples.

AB - BACKGROUND: Globally, there are an estimated 22 million cases of Salmonella enterica serovar Typhi infection each year. However, this figure is likely to be an underestimate due to the low sensitivity of blood culture in S. Typhi diagnosis. The aim of this study was to diagnose S. Typhi by conventional polymerase chain reaction (PCR) using patient's blood preserved with ethylenediamine tetraacetic acid (EDTA).METHODS: From April 2012 to September 2013, typhoid fever surveillance was conducted in Polesgo and Nioko, 2 dry slum areas in Ouagadougou, Burkina Faso. Blood culture was performed for febrile patients using an automated blood culture system. Additional blood was collected in EDTA tubes from those patients and preserved at -80°C. DNA was extracted from EDTA blood and PCR was performed to identify presence of S. Typhi. Randomly selected PCR products were further sequenced to identify S. Typhi-specific amplicons.RESULTS: Of 1674 patients, S. Typhi was isolated from 18 (1.1%) individuals by blood culture. EDTA blood was collected from 1578 patients, of which 298 EDTA samples were tested by PCR. Salmonella Typhi-specific DNA was identified in 44 (14.8%) samples. The sensitivity of S. Typhi-specific PCR from EDTA blood was 89% (74%-100%) among the blood culture-positive cases. Sixteen S. Typhi-positive PCR products were sequenced, and 13 retrieved the sequence of a S. Typhi-specific amplicon.CONCLUSIONS: These findings suggest that blood culture-based diagnoses of S. Typhi underestimate the burden of typhoid fever in Burkina Faso. PCR could be considered as an alternative method for the identification and diagnosis of S. Typhi in blood samples.

KW - Adolescent

KW - Adult

KW - Bacteremia

KW - Burkina Faso

KW - Child

KW - Child, Preschool

KW - Cohort Studies

KW - DNA, Bacterial

KW - Edetic Acid

KW - Female

KW - Fever

KW - Humans

KW - Infant

KW - Infant, Newborn

KW - Male

KW - Molecular Typing

KW - Polymerase Chain Reaction

KW - Public Health Surveillance

KW - Salmonella typhi

KW - Typhoid Fever

KW - Young Adult

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1093/cid/civ770

DO - 10.1093/cid/civ770

M3 - SCORING: Journal article

C2 - 26933018

VL - 62

SP - S37-41

JO - CLIN INFECT DIS

JF - CLIN INFECT DIS

SN - 1058-4838

IS - Suppl 1

ER -