Dexamethasone increases Na+/K+ ATPase activity in insulin secreting cells through SGK1.
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Dexamethasone increases Na+/K+ ATPase activity in insulin secreting cells through SGK1. / Ullrich, Susanne; Zhang, Ying; Avram, Diana; Ranta, Felicia; Kuhl, Dietmar; Häring, Hans-Ulrich; Lang, Florian.
In: BIOCHEM BIOPH RES CO, Vol. 352, No. 3, 3, 2007, p. 662-667.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Dexamethasone increases Na+/K+ ATPase activity in insulin secreting cells through SGK1.
AU - Ullrich, Susanne
AU - Zhang, Ying
AU - Avram, Diana
AU - Ranta, Felicia
AU - Kuhl, Dietmar
AU - Häring, Hans-Ulrich
AU - Lang, Florian
PY - 2007
Y1 - 2007
N2 - Glucocorticoids blunt insulin release, an effect partially due to activation of Kv channels. Similar to those channels Na+/K+ ATPase activity repolarizes the plasma membrane. The present study explored whether glucocorticoids increase the Na+/K+ ATPase activity in pancreatic beta-cells. The glucocorticoid dexamethasone (100 nmol/l for 1 day) significantly increased Na+/K+ ATPase alpha1/beta1-subunit transcript levels and ouabain-sensitive outward current reflecting Na+/K+ ATPase activity in INS-1 cells, effects blunted by glucocorticoid-receptor-blocker RU487 (1 micromol/l). Dexamethasone (100 nmol/l) increased K+ current in beta-cells from wild type mice but not from knockout mice lacking functional serum and glucocorticoid inducible kinase SGK1. Thus, glucocorticoids indeed up-regulate Na+/K+ ATPase activity, an effect requiring SGK1.
AB - Glucocorticoids blunt insulin release, an effect partially due to activation of Kv channels. Similar to those channels Na+/K+ ATPase activity repolarizes the plasma membrane. The present study explored whether glucocorticoids increase the Na+/K+ ATPase activity in pancreatic beta-cells. The glucocorticoid dexamethasone (100 nmol/l for 1 day) significantly increased Na+/K+ ATPase alpha1/beta1-subunit transcript levels and ouabain-sensitive outward current reflecting Na+/K+ ATPase activity in INS-1 cells, effects blunted by glucocorticoid-receptor-blocker RU487 (1 micromol/l). Dexamethasone (100 nmol/l) increased K+ current in beta-cells from wild type mice but not from knockout mice lacking functional serum and glucocorticoid inducible kinase SGK1. Thus, glucocorticoids indeed up-regulate Na+/K+ ATPase activity, an effect requiring SGK1.
M3 - SCORING: Zeitschriftenaufsatz
VL - 352
SP - 662
EP - 667
JO - BIOCHEM BIOPH RES CO
JF - BIOCHEM BIOPH RES CO
SN - 0006-291X
IS - 3
M1 - 3
ER -