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Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing

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Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing. / Gestewitz, Martin; Praetorius, Mark; Albrecht, Tobias.

In: BIOTECH HISTOCHEM, Vol. 97, No. 5, 07.2022, p. 347-354.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Gestewitz, M, Praetorius, M & Albrecht, T 2022, 'Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing', BIOTECH HISTOCHEM, vol. 97, no. 5, pp. 347-354. https://doi.org/10.1080/10520295.2021.1986743

APA

Gestewitz, M., Praetorius, M., & Albrecht, T. (2022). Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing. BIOTECH HISTOCHEM, 97(5), 347-354. https://doi.org/10.1080/10520295.2021.1986743

Vancouver

Gestewitz M, Praetorius M, Albrecht T. Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing. BIOTECH HISTOCHEM. 2022 Jul;97(5):347-354. https://doi.org/10.1080/10520295.2021.1986743

Bibtex

@article{bdef362d782240eeaa24606f7d51abde,
title = "Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing",
abstract = "Owing to the density of the surrounding bone, preparation for histological preparation of the delicate inner ear is difficult and time-consuming; long preparation time increases the risk of deterioration. We present an EDTA decalcification method using an ultrasonic bath and microscopic removal of tissue along defined anatomical landmarks. Our method is rapid and enables preservation of delicate inner ear structures while minimizing the bony capsule. The reduction in volume made possible by our method creates ideal conditions for further processing with digital imaging techniques, in which the resolution is increased with reduced volume.",
author = "Martin Gestewitz and Mark Praetorius and Tobias Albrecht",
year = "2022",
month = jul,
doi = "10.1080/10520295.2021.1986743",
language = "English",
volume = "97",
pages = "347--354",
journal = "BIOTECH HISTOCHEM",
issn = "1052-0295",
publisher = "informa healthcare",
number = "5",

}

RIS

TY - JOUR

T1 - Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing

AU - Gestewitz, Martin

AU - Praetorius, Mark

AU - Albrecht, Tobias

PY - 2022/7

Y1 - 2022/7

N2 - Owing to the density of the surrounding bone, preparation for histological preparation of the delicate inner ear is difficult and time-consuming; long preparation time increases the risk of deterioration. We present an EDTA decalcification method using an ultrasonic bath and microscopic removal of tissue along defined anatomical landmarks. Our method is rapid and enables preservation of delicate inner ear structures while minimizing the bony capsule. The reduction in volume made possible by our method creates ideal conditions for further processing with digital imaging techniques, in which the resolution is increased with reduced volume.

AB - Owing to the density of the surrounding bone, preparation for histological preparation of the delicate inner ear is difficult and time-consuming; long preparation time increases the risk of deterioration. We present an EDTA decalcification method using an ultrasonic bath and microscopic removal of tissue along defined anatomical landmarks. Our method is rapid and enables preservation of delicate inner ear structures while minimizing the bony capsule. The reduction in volume made possible by our method creates ideal conditions for further processing with digital imaging techniques, in which the resolution is increased with reduced volume.

U2 - 10.1080/10520295.2021.1986743

DO - 10.1080/10520295.2021.1986743

M3 - SCORING: Journal article

C2 - 34607476

VL - 97

SP - 347

EP - 354

JO - BIOTECH HISTOCHEM

JF - BIOTECH HISTOCHEM

SN - 1052-0295

IS - 5

ER -