Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing
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Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing. / Gestewitz, Martin; Praetorius, Mark; Albrecht, Tobias.
in: BIOTECH HISTOCHEM, Jahrgang 97, Nr. 5, 07.2022, S. 347-354.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Decalcification method for nondestructive exposure of human inner ear specimens using ultrasonic EDTA scrubbing
AU - Gestewitz, Martin
AU - Praetorius, Mark
AU - Albrecht, Tobias
PY - 2022/7
Y1 - 2022/7
N2 - Owing to the density of the surrounding bone, preparation for histological preparation of the delicate inner ear is difficult and time-consuming; long preparation time increases the risk of deterioration. We present an EDTA decalcification method using an ultrasonic bath and microscopic removal of tissue along defined anatomical landmarks. Our method is rapid and enables preservation of delicate inner ear structures while minimizing the bony capsule. The reduction in volume made possible by our method creates ideal conditions for further processing with digital imaging techniques, in which the resolution is increased with reduced volume.
AB - Owing to the density of the surrounding bone, preparation for histological preparation of the delicate inner ear is difficult and time-consuming; long preparation time increases the risk of deterioration. We present an EDTA decalcification method using an ultrasonic bath and microscopic removal of tissue along defined anatomical landmarks. Our method is rapid and enables preservation of delicate inner ear structures while minimizing the bony capsule. The reduction in volume made possible by our method creates ideal conditions for further processing with digital imaging techniques, in which the resolution is increased with reduced volume.
U2 - 10.1080/10520295.2021.1986743
DO - 10.1080/10520295.2021.1986743
M3 - SCORING: Journal article
C2 - 34607476
VL - 97
SP - 347
EP - 354
JO - BIOTECH HISTOCHEM
JF - BIOTECH HISTOCHEM
SN - 1052-0295
IS - 5
ER -