Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein
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Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein. / Sembritzki, Olivier; Hagel, Christian; Lamszus, Katrin; Deppert, Wolfgang; Bohn, Wolfgang.
In: NEURO-ONCOLOGY, Vol. 4, No. 3, 07.2002, p. 171-8.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein
AU - Sembritzki, Olivier
AU - Hagel, Christian
AU - Lamszus, Katrin
AU - Deppert, Wolfgang
AU - Bohn, Wolfgang
PY - 2002/7
Y1 - 2002/7
N2 - Cytoplasmic accumulation of wild-type p53 in tumor cells indicates that the tumor suppressor is inactive with regard to growth suppressive functions. Whether this occurs randomly during tumor development or characterizes a certain tumor cell subset is not known. Here we assayed primary glioblastomas for expression and subcellular localization of p53 and determined a correlation with expression of intermediate filament proteins characterizing glial cell development. Sixty-nine percent of the tumors were p53 positive in immunohistochemistry. A significant number of tumors (23%) accumulated wild-type p53 in the cytoplasm, which correlated with the presence of vimentin and glial fibrillary acidic protein, except for 1 case. Tumors with exclusive nuclear p53 contained none or only one of these intermediate filament proteins. In an alternative approach, tumors positive for glial fibrillary acidic protein were screened for expression of p53 and vimentin. Thirty-eight percent of these tumors showed cytoplasmic p53, and all of those also expressed vimentin. Tumors with only nuclear p53 were vimentin negative, except for 1 case. No mutation was detected in p53 exons 5 to 8 in tumors with cytoplasmic p53, suggesting that they express wild-type p53. The data indicate that a cytoplasmic accumulation of wild-type p53 in human primary glioblastomas correlates with a certain intermediate filament protein expression, suggesting that it identifies a certain subset of tumors.
AB - Cytoplasmic accumulation of wild-type p53 in tumor cells indicates that the tumor suppressor is inactive with regard to growth suppressive functions. Whether this occurs randomly during tumor development or characterizes a certain tumor cell subset is not known. Here we assayed primary glioblastomas for expression and subcellular localization of p53 and determined a correlation with expression of intermediate filament proteins characterizing glial cell development. Sixty-nine percent of the tumors were p53 positive in immunohistochemistry. A significant number of tumors (23%) accumulated wild-type p53 in the cytoplasm, which correlated with the presence of vimentin and glial fibrillary acidic protein, except for 1 case. Tumors with exclusive nuclear p53 contained none or only one of these intermediate filament proteins. In an alternative approach, tumors positive for glial fibrillary acidic protein were screened for expression of p53 and vimentin. Thirty-eight percent of these tumors showed cytoplasmic p53, and all of those also expressed vimentin. Tumors with only nuclear p53 were vimentin negative, except for 1 case. No mutation was detected in p53 exons 5 to 8 in tumors with cytoplasmic p53, suggesting that they express wild-type p53. The data indicate that a cytoplasmic accumulation of wild-type p53 in human primary glioblastomas correlates with a certain intermediate filament protein expression, suggesting that it identifies a certain subset of tumors.
KW - Active Transport, Cell Nucleus
KW - Adult
KW - Aged
KW - Brain Neoplasms/chemistry
KW - Cell Division
KW - Cell Nucleus/chemistry
KW - Cytoplasm/chemistry
KW - Exons/genetics
KW - Female
KW - Gene Expression Regulation, Neoplastic
KW - Genes, p53
KW - Glial Fibrillary Acidic Protein/analysis
KW - Glioblastoma/chemistry
KW - Humans
KW - Ki-67 Antigen/analysis
KW - Male
KW - Middle Aged
KW - Neoplasm Proteins/analysis
KW - Phenotype
KW - Prognosis
KW - Tumor Suppressor Protein p53/analysis
KW - Vimentin/analysis
U2 - 10.1093/neuonc/4.3.171
DO - 10.1093/neuonc/4.3.171
M3 - SCORING: Journal article
C2 - 12084347
VL - 4
SP - 171
EP - 178
JO - NEURO-ONCOLOGY
JF - NEURO-ONCOLOGY
SN - 1522-8517
IS - 3
ER -