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Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein

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Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein. / Sembritzki, Olivier; Hagel, Christian; Lamszus, Katrin; Deppert, Wolfgang; Bohn, Wolfgang.

in: NEURO-ONCOLOGY, Jahrgang 4, Nr. 3, 07.2002, S. 171-8.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Sembritzki, O, Hagel, C, Lamszus, K, Deppert, W & Bohn, W 2002, 'Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein', NEURO-ONCOLOGY, Jg. 4, Nr. 3, S. 171-8. https://doi.org/10.1093/neuonc/4.3.171

APA

Sembritzki, O., Hagel, C., Lamszus, K., Deppert, W., & Bohn, W. (2002). Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein. NEURO-ONCOLOGY, 4(3), 171-8. https://doi.org/10.1093/neuonc/4.3.171

Vancouver

Sembritzki O, Hagel C, Lamszus K, Deppert W, Bohn W. Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein. NEURO-ONCOLOGY. 2002 Jul;4(3):171-8. https://doi.org/10.1093/neuonc/4.3.171

Bibtex

@article{156109b5e9664d35af92640a86a34c61,
title = "Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein",
abstract = "Cytoplasmic accumulation of wild-type p53 in tumor cells indicates that the tumor suppressor is inactive with regard to growth suppressive functions. Whether this occurs randomly during tumor development or characterizes a certain tumor cell subset is not known. Here we assayed primary glioblastomas for expression and subcellular localization of p53 and determined a correlation with expression of intermediate filament proteins characterizing glial cell development. Sixty-nine percent of the tumors were p53 positive in immunohistochemistry. A significant number of tumors (23%) accumulated wild-type p53 in the cytoplasm, which correlated with the presence of vimentin and glial fibrillary acidic protein, except for 1 case. Tumors with exclusive nuclear p53 contained none or only one of these intermediate filament proteins. In an alternative approach, tumors positive for glial fibrillary acidic protein were screened for expression of p53 and vimentin. Thirty-eight percent of these tumors showed cytoplasmic p53, and all of those also expressed vimentin. Tumors with only nuclear p53 were vimentin negative, except for 1 case. No mutation was detected in p53 exons 5 to 8 in tumors with cytoplasmic p53, suggesting that they express wild-type p53. The data indicate that a cytoplasmic accumulation of wild-type p53 in human primary glioblastomas correlates with a certain intermediate filament protein expression, suggesting that it identifies a certain subset of tumors.",
keywords = "Active Transport, Cell Nucleus, Adult, Aged, Brain Neoplasms/chemistry, Cell Division, Cell Nucleus/chemistry, Cytoplasm/chemistry, Exons/genetics, Female, Gene Expression Regulation, Neoplastic, Genes, p53, Glial Fibrillary Acidic Protein/analysis, Glioblastoma/chemistry, Humans, Ki-67 Antigen/analysis, Male, Middle Aged, Neoplasm Proteins/analysis, Phenotype, Prognosis, Tumor Suppressor Protein p53/analysis, Vimentin/analysis",
author = "Olivier Sembritzki and Christian Hagel and Katrin Lamszus and Wolfgang Deppert and Wolfgang Bohn",
year = "2002",
month = jul,
doi = "10.1093/neuonc/4.3.171",
language = "English",
volume = "4",
pages = "171--8",
journal = "NEURO-ONCOLOGY",
issn = "1522-8517",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Cytoplasmic localization of wild-type p53 in glioblastomas correlates with expression of vimentin and glial fibrillary acidic protein

AU - Sembritzki, Olivier

AU - Hagel, Christian

AU - Lamszus, Katrin

AU - Deppert, Wolfgang

AU - Bohn, Wolfgang

PY - 2002/7

Y1 - 2002/7

N2 - Cytoplasmic accumulation of wild-type p53 in tumor cells indicates that the tumor suppressor is inactive with regard to growth suppressive functions. Whether this occurs randomly during tumor development or characterizes a certain tumor cell subset is not known. Here we assayed primary glioblastomas for expression and subcellular localization of p53 and determined a correlation with expression of intermediate filament proteins characterizing glial cell development. Sixty-nine percent of the tumors were p53 positive in immunohistochemistry. A significant number of tumors (23%) accumulated wild-type p53 in the cytoplasm, which correlated with the presence of vimentin and glial fibrillary acidic protein, except for 1 case. Tumors with exclusive nuclear p53 contained none or only one of these intermediate filament proteins. In an alternative approach, tumors positive for glial fibrillary acidic protein were screened for expression of p53 and vimentin. Thirty-eight percent of these tumors showed cytoplasmic p53, and all of those also expressed vimentin. Tumors with only nuclear p53 were vimentin negative, except for 1 case. No mutation was detected in p53 exons 5 to 8 in tumors with cytoplasmic p53, suggesting that they express wild-type p53. The data indicate that a cytoplasmic accumulation of wild-type p53 in human primary glioblastomas correlates with a certain intermediate filament protein expression, suggesting that it identifies a certain subset of tumors.

AB - Cytoplasmic accumulation of wild-type p53 in tumor cells indicates that the tumor suppressor is inactive with regard to growth suppressive functions. Whether this occurs randomly during tumor development or characterizes a certain tumor cell subset is not known. Here we assayed primary glioblastomas for expression and subcellular localization of p53 and determined a correlation with expression of intermediate filament proteins characterizing glial cell development. Sixty-nine percent of the tumors were p53 positive in immunohistochemistry. A significant number of tumors (23%) accumulated wild-type p53 in the cytoplasm, which correlated with the presence of vimentin and glial fibrillary acidic protein, except for 1 case. Tumors with exclusive nuclear p53 contained none or only one of these intermediate filament proteins. In an alternative approach, tumors positive for glial fibrillary acidic protein were screened for expression of p53 and vimentin. Thirty-eight percent of these tumors showed cytoplasmic p53, and all of those also expressed vimentin. Tumors with only nuclear p53 were vimentin negative, except for 1 case. No mutation was detected in p53 exons 5 to 8 in tumors with cytoplasmic p53, suggesting that they express wild-type p53. The data indicate that a cytoplasmic accumulation of wild-type p53 in human primary glioblastomas correlates with a certain intermediate filament protein expression, suggesting that it identifies a certain subset of tumors.

KW - Active Transport, Cell Nucleus

KW - Adult

KW - Aged

KW - Brain Neoplasms/chemistry

KW - Cell Division

KW - Cell Nucleus/chemistry

KW - Cytoplasm/chemistry

KW - Exons/genetics

KW - Female

KW - Gene Expression Regulation, Neoplastic

KW - Genes, p53

KW - Glial Fibrillary Acidic Protein/analysis

KW - Glioblastoma/chemistry

KW - Humans

KW - Ki-67 Antigen/analysis

KW - Male

KW - Middle Aged

KW - Neoplasm Proteins/analysis

KW - Phenotype

KW - Prognosis

KW - Tumor Suppressor Protein p53/analysis

KW - Vimentin/analysis

U2 - 10.1093/neuonc/4.3.171

DO - 10.1093/neuonc/4.3.171

M3 - SCORING: Journal article

C2 - 12084347

VL - 4

SP - 171

EP - 178

JO - NEURO-ONCOLOGY

JF - NEURO-ONCOLOGY

SN - 1522-8517

IS - 3

ER -