Cutting edge
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Cutting edge : role of B lymphocytes in protective immunity against Salmonella typhimurium infection. / Mittrücker, H W; Raupach, B; Köhler, A; Kaufmann, S H.
In: J IMMUNOL, Vol. 164, No. 4, 15.02.2000, p. 1648-52.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Cutting edge
T2 - role of B lymphocytes in protective immunity against Salmonella typhimurium infection
AU - Mittrücker, H W
AU - Raupach, B
AU - Köhler, A
AU - Kaufmann, S H
PY - 2000/2/15
Y1 - 2000/2/15
N2 - Infection of mice with Salmonella typhimurium gives rise to a disease similar to human typhoid fever caused by S. typhi. Since S. typhimurium is a facultative intracellular bacterium, the requirement of B cells in the immune response against S. typhimurium is a longstanding matter of debate. By infecting mice on a susceptible background and deficient in B cells (Igmu-/- mice) with different strains of S. typhimurium, we could for the first time formally clarify the role of B cells in the response against S. typhimurium. Compared with Igmu+/+ mice, LD50 values in Igmu-/- mice were reduced during primary, and particularly secondary, oral infection with virulent S. typhimurium. After systemic infection, Igmu-/- mice cleared attenuated aroA- S. typhimurium, but vaccine-induced protection against systemic infection with virulent S. typhimurium involved both B cell-dependent and -independent effector mechanisms. Thus, B cell-mediated immunity plays a distinct role in control of S. typhimurium in susceptible mice.
AB - Infection of mice with Salmonella typhimurium gives rise to a disease similar to human typhoid fever caused by S. typhi. Since S. typhimurium is a facultative intracellular bacterium, the requirement of B cells in the immune response against S. typhimurium is a longstanding matter of debate. By infecting mice on a susceptible background and deficient in B cells (Igmu-/- mice) with different strains of S. typhimurium, we could for the first time formally clarify the role of B cells in the response against S. typhimurium. Compared with Igmu+/+ mice, LD50 values in Igmu-/- mice were reduced during primary, and particularly secondary, oral infection with virulent S. typhimurium. After systemic infection, Igmu-/- mice cleared attenuated aroA- S. typhimurium, but vaccine-induced protection against systemic infection with virulent S. typhimurium involved both B cell-dependent and -independent effector mechanisms. Thus, B cell-mediated immunity plays a distinct role in control of S. typhimurium in susceptible mice.
KW - Administration, Oral
KW - Animals
KW - Antibodies, Bacterial
KW - B-Lymphocytes
KW - Immunoglobulin mu-Chains
KW - Injections, Intravenous
KW - Lethal Dose 50
KW - Lymphopenia
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - Salmonella Infections, Animal
KW - Salmonella typhimurium
KW - Typhoid Fever
KW - Typhoid-Paratyphoid Vaccines
KW - Vaccines, Attenuated
KW - Virulence
M3 - SCORING: Journal article
C2 - 10657605
VL - 164
SP - 1648
EP - 1652
JO - J IMMUNOL
JF - J IMMUNOL
SN - 0022-1767
IS - 4
ER -