Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension.
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Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension. / Nagel-Heyer, Stephanie; Goepfert, Christiane; Adamietz, Peter; Meenen, Norbert; Pörtner, Ralf.
In: J BIOTECHNOL, Vol. 121, No. 4, 4, 2006, p. 486-497.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Cultivation of three-dimensional cartilage-carrier-constructs under reduced oxygen tension.
AU - Nagel-Heyer, Stephanie
AU - Goepfert, Christiane
AU - Adamietz, Peter
AU - Meenen, Norbert
AU - Pörtner, Ralf
PY - 2006
Y1 - 2006
N2 - Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. Experiments with different oxygen concentrations (21, 10 and 5%, v/v O(2)) were performed and the constructs were compared qualitatively and quantitatively. The appearance of the cartilage obtained under reduced oxygen tension seemed to be closer to native cartilage with respect to shape of the cells, distribution of the cells within the matrix, smoothness of the surface, etc. The thickness of the cartilage formed by free swelling was always in the same range as for native cartilage (approximately 1mm). Qualitatively the most stable attachment of the cartilage on top of the carrier was found for 10% O(2) (v/v). Especially at 5% O(2) (v/v) the attachment between cartilage and carrier was not sufficient. The constructs generated at lower oxygen tensions had a significantly higher amount of glycosaminoglycan per DNA, but still significantly less when compared to native cartilage. Furthermore, the cultivated cartilage contained a large amount of collagen type II. The experiments proved the applied concept for generation of cartilage-carrier-constructs and the usefulness of cultivation under reduced oxygen tension.
AB - Three-dimensional cartilage-carrier-constructs were produced according to a standard protocol from chondrocytes of an adult mini-pig. Experiments with different oxygen concentrations (21, 10 and 5%, v/v O(2)) were performed and the constructs were compared qualitatively and quantitatively. The appearance of the cartilage obtained under reduced oxygen tension seemed to be closer to native cartilage with respect to shape of the cells, distribution of the cells within the matrix, smoothness of the surface, etc. The thickness of the cartilage formed by free swelling was always in the same range as for native cartilage (approximately 1mm). Qualitatively the most stable attachment of the cartilage on top of the carrier was found for 10% O(2) (v/v). Especially at 5% O(2) (v/v) the attachment between cartilage and carrier was not sufficient. The constructs generated at lower oxygen tensions had a significantly higher amount of glycosaminoglycan per DNA, but still significantly less when compared to native cartilage. Furthermore, the cultivated cartilage contained a large amount of collagen type II. The experiments proved the applied concept for generation of cartilage-carrier-constructs and the usefulness of cultivation under reduced oxygen tension.
KW - Animals
KW - Swine
KW - Swine, Miniature
KW - Cartilage/cytology/physiology
KW - Cell Culture Techniques/methods
KW - Chondrocytes/cytology/physiology
KW - Oxygen
KW - Prostheses and Implants
KW - Tissue Engineering/methods
KW - Animals
KW - Swine
KW - Swine, Miniature
KW - Cartilage/cytology/physiology
KW - Cell Culture Techniques/methods
KW - Chondrocytes/cytology/physiology
KW - Oxygen
KW - Prostheses and Implants
KW - Tissue Engineering/methods
M3 - SCORING: Journal article
VL - 121
SP - 486
EP - 497
JO - J BIOTECHNOL
JF - J BIOTECHNOL
SN - 0168-1656
IS - 4
M1 - 4
ER -
