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Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon

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Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon. / Essone, Paulin N; Adegbite, Bayode R; Mbadinga, Marien J M; Mbouna, Armel V; Lotola-Mougeni, Fabrice; Alabi, Ayodele; Edoa, Jean R; Lell, Bertrand; Alabi, Abraham S; Adegnika, Ayola A; Ramharter, Michael; Siawaya, Joel F D; Grobusch, Martin P; Kremsner, Peter G; Agnandji, Selidji T.

In: INFECTION, Vol. 50, No. 4, 08.2022, p. 897-905.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Essone, PN, Adegbite, BR, Mbadinga, MJM, Mbouna, AV, Lotola-Mougeni, F, Alabi, A, Edoa, JR, Lell, B, Alabi, AS, Adegnika, AA, Ramharter, M, Siawaya, JFD, Grobusch, MP, Kremsner, PG & Agnandji, ST 2022, 'Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon', INFECTION, vol. 50, no. 4, pp. 897-905. https://doi.org/10.1007/s15010-022-01760-8

APA

Essone, P. N., Adegbite, B. R., Mbadinga, M. J. M., Mbouna, A. V., Lotola-Mougeni, F., Alabi, A., Edoa, J. R., Lell, B., Alabi, A. S., Adegnika, A. A., Ramharter, M., Siawaya, J. F. D., Grobusch, M. P., Kremsner, P. G., & Agnandji, S. T. (2022). Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon. INFECTION, 50(4), 897-905. https://doi.org/10.1007/s15010-022-01760-8

Vancouver

Essone PN, Adegbite BR, Mbadinga MJM, Mbouna AV, Lotola-Mougeni F, Alabi A et al. Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon. INFECTION. 2022 Aug;50(4):897-905. https://doi.org/10.1007/s15010-022-01760-8

Bibtex

@article{6ae02fc3a4174fcab7b989159c35bea0,
title = "Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambar{\'e}n{\'e}, Gabon",
abstract = "BACKGROUND: The present study aimed to evaluate the diagnostic utility of creatine kinase-MB (CK-MB), hepcidin (HEPC), phospholipase A2 group IIA (PLa2G2A), and myosin-binding protein C (MYBPC1) for tuberculosis (TB). These four biomarkers are differentially regulated between quiescent Mycobacterium tuberculosis (Mtb) infected individuals (non-progressors to TB disease) and Mtb-infected TB disease progressors 6 months before the onset of symptoms.METHODS: We enrolled samples from patients experiencing moderate-to-severe pulmonary infections diseases including 23 TB cases confirmed by smear microscopy and culture, and 34 TB-negative cases. For each participant, the serum levels of the four biomarkers were measured using ELISA.RESULTS: The levels of CK-MB and HEPC were significantly reduced in patients with active TB disease. CK-MB median level was 2045 pg/ml (1455-4000 pg/ml) in active TB cases and 3245 pg/ml (1645-4000 pg/ml) in non-TB pulmonary diseases. Using the receiver operating characteristic curve (ROC) analysis, HEPC and CK-MB had the Area Under the Curve (AUC) of 79% (95% CI 67-91%) and 81% (95% CI 69-93%), respectively. Both markers correlated with TB diagnosis as a single marker. PLa2G2A and MYBPC1 with AUCs of 48% (95% CI 36-65%) and 62% (95% CI 48-76%) did not performed well as single biomarkers. The three markers'model (CK-MB-HEPC-PLa2G2A) had the highest diagnostic accuracy at 82% (95% CI 56-82%) after cross-validation.CONCLUSION: CK-MB and HEPC levels were statistically different between confirmed TB cases and non-TB cases. This study yields promising results for the rapid diagnosis of TB disease using a single marker or three biomarkers model.",
keywords = "Biomarkers, Creatine Kinase, MB Form, Early Diagnosis, Gabon, Hepcidins, Humans, Mycobacterium tuberculosis, ROC Curve, Sensitivity and Specificity, Tuberculosis/diagnosis, Tuberculosis, Pulmonary/diagnosis",
author = "Essone, {Paulin N} and Adegbite, {Bayode R} and Mbadinga, {Marien J M} and Mbouna, {Armel V} and Fabrice Lotola-Mougeni and Ayodele Alabi and Edoa, {Jean R} and Bertrand Lell and Alabi, {Abraham S} and Adegnika, {Ayola A} and Michael Ramharter and Siawaya, {Joel F D} and Grobusch, {Martin P} and Kremsner, {Peter G} and Agnandji, {Selidji T}",
note = "{\textcopyright} 2022. The Author(s).",
year = "2022",
month = aug,
doi = "10.1007/s15010-022-01760-8",
language = "English",
volume = "50",
pages = "897--905",
journal = "INFECTION",
issn = "0300-8126",
publisher = "Urban und Vogel",
number = "4",

}

RIS

TY - JOUR

T1 - Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon

AU - Essone, Paulin N

AU - Adegbite, Bayode R

AU - Mbadinga, Marien J M

AU - Mbouna, Armel V

AU - Lotola-Mougeni, Fabrice

AU - Alabi, Ayodele

AU - Edoa, Jean R

AU - Lell, Bertrand

AU - Alabi, Abraham S

AU - Adegnika, Ayola A

AU - Ramharter, Michael

AU - Siawaya, Joel F D

AU - Grobusch, Martin P

AU - Kremsner, Peter G

AU - Agnandji, Selidji T

N1 - © 2022. The Author(s).

PY - 2022/8

Y1 - 2022/8

N2 - BACKGROUND: The present study aimed to evaluate the diagnostic utility of creatine kinase-MB (CK-MB), hepcidin (HEPC), phospholipase A2 group IIA (PLa2G2A), and myosin-binding protein C (MYBPC1) for tuberculosis (TB). These four biomarkers are differentially regulated between quiescent Mycobacterium tuberculosis (Mtb) infected individuals (non-progressors to TB disease) and Mtb-infected TB disease progressors 6 months before the onset of symptoms.METHODS: We enrolled samples from patients experiencing moderate-to-severe pulmonary infections diseases including 23 TB cases confirmed by smear microscopy and culture, and 34 TB-negative cases. For each participant, the serum levels of the four biomarkers were measured using ELISA.RESULTS: The levels of CK-MB and HEPC were significantly reduced in patients with active TB disease. CK-MB median level was 2045 pg/ml (1455-4000 pg/ml) in active TB cases and 3245 pg/ml (1645-4000 pg/ml) in non-TB pulmonary diseases. Using the receiver operating characteristic curve (ROC) analysis, HEPC and CK-MB had the Area Under the Curve (AUC) of 79% (95% CI 67-91%) and 81% (95% CI 69-93%), respectively. Both markers correlated with TB diagnosis as a single marker. PLa2G2A and MYBPC1 with AUCs of 48% (95% CI 36-65%) and 62% (95% CI 48-76%) did not performed well as single biomarkers. The three markers'model (CK-MB-HEPC-PLa2G2A) had the highest diagnostic accuracy at 82% (95% CI 56-82%) after cross-validation.CONCLUSION: CK-MB and HEPC levels were statistically different between confirmed TB cases and non-TB cases. This study yields promising results for the rapid diagnosis of TB disease using a single marker or three biomarkers model.

AB - BACKGROUND: The present study aimed to evaluate the diagnostic utility of creatine kinase-MB (CK-MB), hepcidin (HEPC), phospholipase A2 group IIA (PLa2G2A), and myosin-binding protein C (MYBPC1) for tuberculosis (TB). These four biomarkers are differentially regulated between quiescent Mycobacterium tuberculosis (Mtb) infected individuals (non-progressors to TB disease) and Mtb-infected TB disease progressors 6 months before the onset of symptoms.METHODS: We enrolled samples from patients experiencing moderate-to-severe pulmonary infections diseases including 23 TB cases confirmed by smear microscopy and culture, and 34 TB-negative cases. For each participant, the serum levels of the four biomarkers were measured using ELISA.RESULTS: The levels of CK-MB and HEPC were significantly reduced in patients with active TB disease. CK-MB median level was 2045 pg/ml (1455-4000 pg/ml) in active TB cases and 3245 pg/ml (1645-4000 pg/ml) in non-TB pulmonary diseases. Using the receiver operating characteristic curve (ROC) analysis, HEPC and CK-MB had the Area Under the Curve (AUC) of 79% (95% CI 67-91%) and 81% (95% CI 69-93%), respectively. Both markers correlated with TB diagnosis as a single marker. PLa2G2A and MYBPC1 with AUCs of 48% (95% CI 36-65%) and 62% (95% CI 48-76%) did not performed well as single biomarkers. The three markers'model (CK-MB-HEPC-PLa2G2A) had the highest diagnostic accuracy at 82% (95% CI 56-82%) after cross-validation.CONCLUSION: CK-MB and HEPC levels were statistically different between confirmed TB cases and non-TB cases. This study yields promising results for the rapid diagnosis of TB disease using a single marker or three biomarkers model.

KW - Biomarkers

KW - Creatine Kinase, MB Form

KW - Early Diagnosis

KW - Gabon

KW - Hepcidins

KW - Humans

KW - Mycobacterium tuberculosis

KW - ROC Curve

KW - Sensitivity and Specificity

KW - Tuberculosis/diagnosis

KW - Tuberculosis, Pulmonary/diagnosis

U2 - 10.1007/s15010-022-01760-8

DO - 10.1007/s15010-022-01760-8

M3 - SCORING: Journal article

C2 - 35133607

VL - 50

SP - 897

EP - 905

JO - INFECTION

JF - INFECTION

SN - 0300-8126

IS - 4

ER -