Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon
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Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon. / Essone, Paulin N; Adegbite, Bayode R; Mbadinga, Marien J M; Mbouna, Armel V; Lotola-Mougeni, Fabrice; Alabi, Ayodele; Edoa, Jean R; Lell, Bertrand; Alabi, Abraham S; Adegnika, Ayola A; Ramharter, Michael; Siawaya, Joel F D; Grobusch, Martin P; Kremsner, Peter G; Agnandji, Selidji T.
in: INFECTION, Jahrgang 50, Nr. 4, 08.2022, S. 897-905.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Creatine kinase-(MB) and hepcidin as candidate biomarkers for early diagnosis of pulmonary tuberculosis: a proof-of-concept study in Lambaréné, Gabon
AU - Essone, Paulin N
AU - Adegbite, Bayode R
AU - Mbadinga, Marien J M
AU - Mbouna, Armel V
AU - Lotola-Mougeni, Fabrice
AU - Alabi, Ayodele
AU - Edoa, Jean R
AU - Lell, Bertrand
AU - Alabi, Abraham S
AU - Adegnika, Ayola A
AU - Ramharter, Michael
AU - Siawaya, Joel F D
AU - Grobusch, Martin P
AU - Kremsner, Peter G
AU - Agnandji, Selidji T
N1 - © 2022. The Author(s).
PY - 2022/8
Y1 - 2022/8
N2 - BACKGROUND: The present study aimed to evaluate the diagnostic utility of creatine kinase-MB (CK-MB), hepcidin (HEPC), phospholipase A2 group IIA (PLa2G2A), and myosin-binding protein C (MYBPC1) for tuberculosis (TB). These four biomarkers are differentially regulated between quiescent Mycobacterium tuberculosis (Mtb) infected individuals (non-progressors to TB disease) and Mtb-infected TB disease progressors 6 months before the onset of symptoms.METHODS: We enrolled samples from patients experiencing moderate-to-severe pulmonary infections diseases including 23 TB cases confirmed by smear microscopy and culture, and 34 TB-negative cases. For each participant, the serum levels of the four biomarkers were measured using ELISA.RESULTS: The levels of CK-MB and HEPC were significantly reduced in patients with active TB disease. CK-MB median level was 2045 pg/ml (1455-4000 pg/ml) in active TB cases and 3245 pg/ml (1645-4000 pg/ml) in non-TB pulmonary diseases. Using the receiver operating characteristic curve (ROC) analysis, HEPC and CK-MB had the Area Under the Curve (AUC) of 79% (95% CI 67-91%) and 81% (95% CI 69-93%), respectively. Both markers correlated with TB diagnosis as a single marker. PLa2G2A and MYBPC1 with AUCs of 48% (95% CI 36-65%) and 62% (95% CI 48-76%) did not performed well as single biomarkers. The three markers'model (CK-MB-HEPC-PLa2G2A) had the highest diagnostic accuracy at 82% (95% CI 56-82%) after cross-validation.CONCLUSION: CK-MB and HEPC levels were statistically different between confirmed TB cases and non-TB cases. This study yields promising results for the rapid diagnosis of TB disease using a single marker or three biomarkers model.
AB - BACKGROUND: The present study aimed to evaluate the diagnostic utility of creatine kinase-MB (CK-MB), hepcidin (HEPC), phospholipase A2 group IIA (PLa2G2A), and myosin-binding protein C (MYBPC1) for tuberculosis (TB). These four biomarkers are differentially regulated between quiescent Mycobacterium tuberculosis (Mtb) infected individuals (non-progressors to TB disease) and Mtb-infected TB disease progressors 6 months before the onset of symptoms.METHODS: We enrolled samples from patients experiencing moderate-to-severe pulmonary infections diseases including 23 TB cases confirmed by smear microscopy and culture, and 34 TB-negative cases. For each participant, the serum levels of the four biomarkers were measured using ELISA.RESULTS: The levels of CK-MB and HEPC were significantly reduced in patients with active TB disease. CK-MB median level was 2045 pg/ml (1455-4000 pg/ml) in active TB cases and 3245 pg/ml (1645-4000 pg/ml) in non-TB pulmonary diseases. Using the receiver operating characteristic curve (ROC) analysis, HEPC and CK-MB had the Area Under the Curve (AUC) of 79% (95% CI 67-91%) and 81% (95% CI 69-93%), respectively. Both markers correlated with TB diagnosis as a single marker. PLa2G2A and MYBPC1 with AUCs of 48% (95% CI 36-65%) and 62% (95% CI 48-76%) did not performed well as single biomarkers. The three markers'model (CK-MB-HEPC-PLa2G2A) had the highest diagnostic accuracy at 82% (95% CI 56-82%) after cross-validation.CONCLUSION: CK-MB and HEPC levels were statistically different between confirmed TB cases and non-TB cases. This study yields promising results for the rapid diagnosis of TB disease using a single marker or three biomarkers model.
KW - Biomarkers
KW - Creatine Kinase, MB Form
KW - Early Diagnosis
KW - Gabon
KW - Hepcidins
KW - Humans
KW - Mycobacterium tuberculosis
KW - ROC Curve
KW - Sensitivity and Specificity
KW - Tuberculosis/diagnosis
KW - Tuberculosis, Pulmonary/diagnosis
U2 - 10.1007/s15010-022-01760-8
DO - 10.1007/s15010-022-01760-8
M3 - SCORING: Journal article
C2 - 35133607
VL - 50
SP - 897
EP - 905
JO - INFECTION
JF - INFECTION
SN - 0300-8126
IS - 4
ER -