Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19
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Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19. / Majstrowicz, Katarzyna; Honnert, Ulrike; Nikolaus, Petra; Schwarz, Vera; Oeding, Stefanie J; Hemkemeyer, Sandra A; Bähler, Martin.
In: J CELL SCI, Vol. 134, No. 10, 15.05.2021, p. 1-18.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19
AU - Majstrowicz, Katarzyna
AU - Honnert, Ulrike
AU - Nikolaus, Petra
AU - Schwarz, Vera
AU - Oeding, Stefanie J
AU - Hemkemeyer, Sandra A
AU - Bähler, Martin
N1 - © 2021. Published by The Company of Biologists Ltd.
PY - 2021/5/15
Y1 - 2021/5/15
N2 - Myosin XIX (Myo19) is an actin-based motor that competes with adaptors of microtubule-based motors for binding to the outer mitochondrial transmembrane proteins Miro1 and Miro2 (collectively Miro, also known as RhoT1 and RhoT2, respectively). Here, we investigate which mitochondrial and cellular processes depend on the coordination of Myo19 and microtubule-based motor activities. To this end, we created Myo19-deficient HEK293T cells. Mitochondria in these cells were not properly fragmented at mitosis and were partitioned asymmetrically to daughter cells. Respiratory functions of mitochondria were impaired and ROS generation was enhanced. On a cellular level, cell proliferation, cytokinesis and cell-matrix adhesion were negatively affected. On a molecular level, Myo19 regulates focal adhesions in interphase, and mitochondrial fusion and mitochondrially associated levels of fission protein Drp1 and adaptor proteins dynactin and TRAK1 at prometaphase. These alterations were due to a disturbed coordination of Myo19 and microtubule-based motor activities by Miro.
AB - Myosin XIX (Myo19) is an actin-based motor that competes with adaptors of microtubule-based motors for binding to the outer mitochondrial transmembrane proteins Miro1 and Miro2 (collectively Miro, also known as RhoT1 and RhoT2, respectively). Here, we investigate which mitochondrial and cellular processes depend on the coordination of Myo19 and microtubule-based motor activities. To this end, we created Myo19-deficient HEK293T cells. Mitochondria in these cells were not properly fragmented at mitosis and were partitioned asymmetrically to daughter cells. Respiratory functions of mitochondria were impaired and ROS generation was enhanced. On a cellular level, cell proliferation, cytokinesis and cell-matrix adhesion were negatively affected. On a molecular level, Myo19 regulates focal adhesions in interphase, and mitochondrial fusion and mitochondrially associated levels of fission protein Drp1 and adaptor proteins dynactin and TRAK1 at prometaphase. These alterations were due to a disturbed coordination of Myo19 and microtubule-based motor activities by Miro.
KW - Actins/genetics
KW - HEK293 Cells
KW - Humans
KW - Mitochondria/metabolism
KW - Mitochondrial Dynamics
KW - Mitochondrial Membranes/metabolism
KW - Mitochondrial Proteins/genetics
KW - Myosins/metabolism
KW - rho GTP-Binding Proteins/metabolism
U2 - 10.1242/jcs.255844
DO - 10.1242/jcs.255844
M3 - SCORING: Journal article
C2 - 34013964
VL - 134
SP - 1
EP - 18
JO - J CELL SCI
JF - J CELL SCI
SN - 0021-9533
IS - 10
ER -