Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19

Katarzyna Majstrowicz; Ulrike Honnert; Petra Nikolaus; Vera Schwarz; Stefanie J Oeding; Sandra A Hemkemeyer; Martin Bähler

doi:10.1242/jcs.255844

Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19

Standard

Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19. / Majstrowicz, Katarzyna; Honnert, Ulrike; Nikolaus, Petra; Schwarz, Vera; Oeding, Stefanie J; Hemkemeyer, Sandra A; Bähler, Martin.

in: J CELL SCI, Jahrgang 134, Nr. 10, 15.05.2021, S. 1-18.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Majstrowicz, K, Honnert, U, Nikolaus, P, Schwarz, V, Oeding, SJ, Hemkemeyer, SA & Bähler, M 2021, 'Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19', J CELL SCI, Jg. 134, Nr. 10, S. 1-18. https://doi.org/10.1242/jcs.255844

APA

Majstrowicz, K., Honnert, U., Nikolaus, P., Schwarz, V., Oeding, S. J., Hemkemeyer, S. A., & Bähler, M. (2021). Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19. J CELL SCI, 134(10), 1-18. https://doi.org/10.1242/jcs.255844

Vancouver

Majstrowicz K, Honnert U, Nikolaus P, Schwarz V, Oeding SJ, Hemkemeyer SA et al. Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19. J CELL SCI. 2021 Mai 15;134(10):1-18. https://doi.org/10.1242/jcs.255844

Bibtex

@article{78a515784f46409b9729b1ff4df1d2b1,

title = "Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19",

abstract = "Myosin XIX (Myo19) is an actin-based motor that competes with adaptors of microtubule-based motors for binding to the outer mitochondrial transmembrane proteins Miro1 and Miro2 (collectively Miro, also known as RhoT1 and RhoT2, respectively). Here, we investigate which mitochondrial and cellular processes depend on the coordination of Myo19 and microtubule-based motor activities. To this end, we created Myo19-deficient HEK293T cells. Mitochondria in these cells were not properly fragmented at mitosis and were partitioned asymmetrically to daughter cells. Respiratory functions of mitochondria were impaired and ROS generation was enhanced. On a cellular level, cell proliferation, cytokinesis and cell-matrix adhesion were negatively affected. On a molecular level, Myo19 regulates focal adhesions in interphase, and mitochondrial fusion and mitochondrially associated levels of fission protein Drp1 and adaptor proteins dynactin and TRAK1 at prometaphase. These alterations were due to a disturbed coordination of Myo19 and microtubule-based motor activities by Miro.",

keywords = "Actins/genetics, HEK293 Cells, Humans, Mitochondria/metabolism, Mitochondrial Dynamics, Mitochondrial Membranes/metabolism, Mitochondrial Proteins/genetics, Myosins/metabolism, rho GTP-Binding Proteins/metabolism",

author = "Katarzyna Majstrowicz and Ulrike Honnert and Petra Nikolaus and Vera Schwarz and Oeding, {Stefanie J} and Hemkemeyer, {Sandra A} and Martin B{\"a}hler",

note = "{\textcopyright} 2021. Published by The Company of Biologists Ltd.",

year = "2021",

month = may,

day = "15",

doi = "10.1242/jcs.255844",

language = "English",

volume = "134",

pages = "1--18",

journal = "J CELL SCI",

issn = "0021-9533",

publisher = "Company of Biologists Ltd",

number = "10",

}

RIS

TY - JOUR

T1 - Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19

AU - Majstrowicz, Katarzyna

AU - Honnert, Ulrike

AU - Nikolaus, Petra

AU - Schwarz, Vera

AU - Oeding, Stefanie J

AU - Hemkemeyer, Sandra A

AU - Bähler, Martin

PY - 2021/5/15

Y1 - 2021/5/15

N2 - Myosin XIX (Myo19) is an actin-based motor that competes with adaptors of microtubule-based motors for binding to the outer mitochondrial transmembrane proteins Miro1 and Miro2 (collectively Miro, also known as RhoT1 and RhoT2, respectively). Here, we investigate which mitochondrial and cellular processes depend on the coordination of Myo19 and microtubule-based motor activities. To this end, we created Myo19-deficient HEK293T cells. Mitochondria in these cells were not properly fragmented at mitosis and were partitioned asymmetrically to daughter cells. Respiratory functions of mitochondria were impaired and ROS generation was enhanced. On a cellular level, cell proliferation, cytokinesis and cell-matrix adhesion were negatively affected. On a molecular level, Myo19 regulates focal adhesions in interphase, and mitochondrial fusion and mitochondrially associated levels of fission protein Drp1 and adaptor proteins dynactin and TRAK1 at prometaphase. These alterations were due to a disturbed coordination of Myo19 and microtubule-based motor activities by Miro.

AB - Myosin XIX (Myo19) is an actin-based motor that competes with adaptors of microtubule-based motors for binding to the outer mitochondrial transmembrane proteins Miro1 and Miro2 (collectively Miro, also known as RhoT1 and RhoT2, respectively). Here, we investigate which mitochondrial and cellular processes depend on the coordination of Myo19 and microtubule-based motor activities. To this end, we created Myo19-deficient HEK293T cells. Mitochondria in these cells were not properly fragmented at mitosis and were partitioned asymmetrically to daughter cells. Respiratory functions of mitochondria were impaired and ROS generation was enhanced. On a cellular level, cell proliferation, cytokinesis and cell-matrix adhesion were negatively affected. On a molecular level, Myo19 regulates focal adhesions in interphase, and mitochondrial fusion and mitochondrially associated levels of fission protein Drp1 and adaptor proteins dynactin and TRAK1 at prometaphase. These alterations were due to a disturbed coordination of Myo19 and microtubule-based motor activities by Miro.

KW - Actins/genetics

KW - HEK293 Cells

KW - Humans

KW - Mitochondria/metabolism

KW - Mitochondrial Dynamics

KW - Mitochondrial Membranes/metabolism

KW - Mitochondrial Proteins/genetics

KW - Myosins/metabolism

KW - rho GTP-Binding Proteins/metabolism

U2 - 10.1242/jcs.255844

DO - 10.1242/jcs.255844

M3 - SCORING: Journal article

C2 - 34013964

VL - 134

SP - 1

EP - 18

JO - J CELL SCI

JF - J CELL SCI

SN - 0021-9533

IS - 10

ER -