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Control of type III protein secretion using a minimal genetic system

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Control of type III protein secretion using a minimal genetic system. / Song, Miryoung; Sukovich, David J; Ciccarelli, Luciano; Mayr, Julia; Fernandez-Rodriguez, Jesus; Mirsky, Ethan A; Tucker, Alex C; Gordon, D Benjamin; Marlovits, Thomas C; Voigt, Christopher A.

In: NAT COMMUN, Vol. 8, 09.05.2017, p. 14737.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Song, M, Sukovich, DJ, Ciccarelli, L, Mayr, J, Fernandez-Rodriguez, J, Mirsky, EA, Tucker, AC, Gordon, DB, Marlovits, TC & Voigt, CA 2017, 'Control of type III protein secretion using a minimal genetic system', NAT COMMUN, vol. 8, pp. 14737. https://doi.org/10.1038/ncomms14737

APA

Song, M., Sukovich, D. J., Ciccarelli, L., Mayr, J., Fernandez-Rodriguez, J., Mirsky, E. A., Tucker, A. C., Gordon, D. B., Marlovits, T. C., & Voigt, C. A. (2017). Control of type III protein secretion using a minimal genetic system. NAT COMMUN, 8, 14737. https://doi.org/10.1038/ncomms14737

Vancouver

Song M, Sukovich DJ, Ciccarelli L, Mayr J, Fernandez-Rodriguez J, Mirsky EA et al. Control of type III protein secretion using a minimal genetic system. NAT COMMUN. 2017 May 9;8:14737. https://doi.org/10.1038/ncomms14737

Bibtex

@article{f3d9818a11514a32ace2cf8f10dd76ce,
title = "Control of type III protein secretion using a minimal genetic system",
abstract = "Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.",
keywords = "Journal Article",
author = "Miryoung Song and Sukovich, {David J} and Luciano Ciccarelli and Julia Mayr and Jesus Fernandez-Rodriguez and Mirsky, {Ethan A} and Tucker, {Alex C} and Gordon, {D Benjamin} and Marlovits, {Thomas C} and Voigt, {Christopher A}",
year = "2017",
month = may,
day = "9",
doi = "10.1038/ncomms14737",
language = "English",
volume = "8",
pages = "14737",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Control of type III protein secretion using a minimal genetic system

AU - Song, Miryoung

AU - Sukovich, David J

AU - Ciccarelli, Luciano

AU - Mayr, Julia

AU - Fernandez-Rodriguez, Jesus

AU - Mirsky, Ethan A

AU - Tucker, Alex C

AU - Gordon, D Benjamin

AU - Marlovits, Thomas C

AU - Voigt, Christopher A

PY - 2017/5/9

Y1 - 2017/5/9

N2 - Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.

AB - Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.

KW - Journal Article

U2 - 10.1038/ncomms14737

DO - 10.1038/ncomms14737

M3 - SCORING: Journal article

C2 - 28485369

VL - 8

SP - 14737

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

ER -