Control of type III protein secretion using a minimal genetic system
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Control of type III protein secretion using a minimal genetic system. / Song, Miryoung; Sukovich, David J; Ciccarelli, Luciano; Mayr, Julia; Fernandez-Rodriguez, Jesus; Mirsky, Ethan A; Tucker, Alex C; Gordon, D Benjamin; Marlovits, Thomas C; Voigt, Christopher A.
in: NAT COMMUN, Jahrgang 8, 09.05.2017, S. 14737.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Control of type III protein secretion using a minimal genetic system
AU - Song, Miryoung
AU - Sukovich, David J
AU - Ciccarelli, Luciano
AU - Mayr, Julia
AU - Fernandez-Rodriguez, Jesus
AU - Mirsky, Ethan A
AU - Tucker, Alex C
AU - Gordon, D Benjamin
AU - Marlovits, Thomas C
AU - Voigt, Christopher A
PY - 2017/5/9
Y1 - 2017/5/9
N2 - Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.
AB - Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.
KW - Journal Article
U2 - 10.1038/ncomms14737
DO - 10.1038/ncomms14737
M3 - SCORING: Journal article
C2 - 28485369
VL - 8
SP - 14737
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
ER -