Compartment specific expression of dendritic cell markers in human glomerulonephritis
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Compartment specific expression of dendritic cell markers in human glomerulonephritis. / Segerer, S; Heller, F; Lindenmeyer, M T; Schmid, H; Cohen, C D; Draganovici, D; Mandelbaum, J; Nelson, P J; Gröne, H-J; Gröne, E F; Figel, A-M; Nössner, E; Schlöndorff, D.
In: KIDNEY INT, Vol. 74, No. 1, 07.2008, p. 37-46.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Compartment specific expression of dendritic cell markers in human glomerulonephritis
AU - Segerer, S
AU - Heller, F
AU - Lindenmeyer, M T
AU - Schmid, H
AU - Cohen, C D
AU - Draganovici, D
AU - Mandelbaum, J
AU - Nelson, P J
AU - Gröne, H-J
AU - Gröne, E F
AU - Figel, A-M
AU - Nössner, E
AU - Schlöndorff, D
PY - 2008/7
Y1 - 2008/7
N2 - Macrophages and dendritic cells are heterogenous and highly plastic bone marrow-derived cells that play major roles in renal diseases. We characterized these cells using immunohistochemistry in 55 renal biopsies from control patients or patients with glomerulonephritis as an initial step towards postulating specific roles for these cells in kidney disease. In proliferative glomerulonephritis numerous CD68 positive (pan monocyte, macrophage and dendritic marker) cells were found in both glomeruli and the tubulointerstitial space, however, a myeloid dendritic cell marker (DC-SIGN) was identified only in the tubulointerstitium. A significant number of plasmacytoid dendritic cells (identified as BDCA-2 positive cells) were seen at sites of interstitial inflammation, including follicular aggregates of inflammatory cells. Langerin positive cells (a marker of Langerhans' cells) were detectable but rare. The area of either CD68 or DC-SIGN positive interstitial cells correlated with serum creatinine. Low levels of DC-SIGN, DC-LAMP and MHC class II mRNA were present in the tubulointerstitial space in controls and increased only in that region in proliferative glomerulonephritis. We demonstrate that the CD68 positive cells infiltrating the glomerulus lack dendritic cell markers (reflecting macrophages), whereas in the tubulointerstitial space the majority of CD68 positive cells are also DC-SIGN positive (reflecting myeloid dendritic cells). Their number correlated with serum creatinine, which further emphasizes the significance of interstitial DCs in progressive glomerular diseases.
AB - Macrophages and dendritic cells are heterogenous and highly plastic bone marrow-derived cells that play major roles in renal diseases. We characterized these cells using immunohistochemistry in 55 renal biopsies from control patients or patients with glomerulonephritis as an initial step towards postulating specific roles for these cells in kidney disease. In proliferative glomerulonephritis numerous CD68 positive (pan monocyte, macrophage and dendritic marker) cells were found in both glomeruli and the tubulointerstitial space, however, a myeloid dendritic cell marker (DC-SIGN) was identified only in the tubulointerstitium. A significant number of plasmacytoid dendritic cells (identified as BDCA-2 positive cells) were seen at sites of interstitial inflammation, including follicular aggregates of inflammatory cells. Langerin positive cells (a marker of Langerhans' cells) were detectable but rare. The area of either CD68 or DC-SIGN positive interstitial cells correlated with serum creatinine. Low levels of DC-SIGN, DC-LAMP and MHC class II mRNA were present in the tubulointerstitial space in controls and increased only in that region in proliferative glomerulonephritis. We demonstrate that the CD68 positive cells infiltrating the glomerulus lack dendritic cell markers (reflecting macrophages), whereas in the tubulointerstitial space the majority of CD68 positive cells are also DC-SIGN positive (reflecting myeloid dendritic cells). Their number correlated with serum creatinine, which further emphasizes the significance of interstitial DCs in progressive glomerular diseases.
KW - Adolescent
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Antigens, CD
KW - Antigens, Differentiation, Myelomonocytic
KW - Biomarkers
KW - Case-Control Studies
KW - Cell Adhesion Molecules
KW - Cell Movement
KW - Dendritic Cells
KW - Disease Progression
KW - Glomerulonephritis
KW - Humans
KW - Immunohistochemistry
KW - Immunophenotyping
KW - Inflammation
KW - Kidney Glomerulus
KW - Lectins, C-Type
KW - Middle Aged
KW - Receptors, Cell Surface
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1038/ki.2008.99
DO - 10.1038/ki.2008.99
M3 - SCORING: Journal article
C2 - 18368027
VL - 74
SP - 37
EP - 46
JO - KIDNEY INT
JF - KIDNEY INT
SN - 0085-2538
IS - 1
ER -