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Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology

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Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology. / Bonifay, Amandine; Mackman, Nigel; Hisada, Yohei; Sachetto, Ana Teresa Azevedo; Hau, Chi; Gray, Elaine; Hogwood, John; Aharon, Anat; Badimon, Lina; Barile, Lucio; Baudar, Justine; Beckmann, Lennart; Benedikter, Birke; Bolis, Sara; Bouriche, Tarik; Brambilla, Marta; Burrello, Jacopo; Camera, Marina; Campello, Elena; Ettelaie, Camille; Faille, Dorothée; Featherby, Sophie; Franco, Corentin; Guldenpfennig, Maite; Hansen, John-Bjarne; Judicone, Coralie; Kim, Yohan; Kristensen, Soren Risom; Laakmann, Katrin; Langer, Florian; Latysheva, Nadezhda; Lucien, Fabrice; de Menezes, Erika Marques; Mullier, François; Norris, Philip; Nybo, Jette; Orbe, Josune; Osterud, Bjarne; Paramo, Jose A; Radu, Claudia M; Roncal, Carmen; Samadi, Nazanin; Snir, Omri; Suades, Rosa; Wahlund, Casper; Chareyre, Corinne; Abdili, Evelyne; Martinod, Kimberly; Thaler, Johannes; Dignat-George, Françoise; Nieuwland, Rienk; Lacroix, Romaric.

In: J THROMB HAEMOST, Vol. 22, No. 10, 10.2024, p. 2910-2921.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bonifay, A, Mackman, N, Hisada, Y, Sachetto, ATA, Hau, C, Gray, E, Hogwood, J, Aharon, A, Badimon, L, Barile, L, Baudar, J, Beckmann, L, Benedikter, B, Bolis, S, Bouriche, T, Brambilla, M, Burrello, J, Camera, M, Campello, E, Ettelaie, C, Faille, D, Featherby, S, Franco, C, Guldenpfennig, M, Hansen, J-B, Judicone, C, Kim, Y, Kristensen, SR, Laakmann, K, Langer, F, Latysheva, N, Lucien, F, de Menezes, EM, Mullier, F, Norris, P, Nybo, J, Orbe, J, Osterud, B, Paramo, JA, Radu, CM, Roncal, C, Samadi, N, Snir, O, Suades, R, Wahlund, C, Chareyre, C, Abdili, E, Martinod, K, Thaler, J, Dignat-George, F, Nieuwland, R & Lacroix, R 2024, 'Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology', J THROMB HAEMOST, vol. 22, no. 10, pp. 2910-2921. https://doi.org/10.1016/j.jtha.2024.05.037

APA

Bonifay, A., Mackman, N., Hisada, Y., Sachetto, A. T. A., Hau, C., Gray, E., Hogwood, J., Aharon, A., Badimon, L., Barile, L., Baudar, J., Beckmann, L., Benedikter, B., Bolis, S., Bouriche, T., Brambilla, M., Burrello, J., Camera, M., Campello, E., ... Lacroix, R. (2024). Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology. J THROMB HAEMOST, 22(10), 2910-2921. https://doi.org/10.1016/j.jtha.2024.05.037

Vancouver

Bonifay A, Mackman N, Hisada Y, Sachetto ATA, Hau C, Gray E et al. Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology. J THROMB HAEMOST. 2024 Oct;22(10):2910-2921. https://doi.org/10.1016/j.jtha.2024.05.037

Bibtex

@article{c36f2f2655744694bf24557669576ef2,
title = "Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology",
abstract = "BACKGROUND: Scientific and clinical interest in extracellular vesicles (EVs) is growing. EVs that expose tissue factor (TF) bind factor VII/VIIa and can trigger coagulation. Highly procoagulant TF-exposing EVs are detectable in the circulation in various diseases, such as sepsis, COVID-19, or cancer. Many in-house and commercially available assays have been developed to measure EV-TF activity and antigen, but only a few studies have compared some of these assays.OBJECTIVES: The International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Subcommittee on Vascular Biology initiated a multicenter study to compare the sensitivity, specificity, and reproducibility of these assays.METHODS: Platelet-depleted plasma samples were prepared from blood of healthy donors. The plasma samples were spiked either with EVs from human milk or EVs from TF-positive and TF-negative cell lines. Plasma was also prepared from whole human blood with or without lipopolysaccharide stimulation. Twenty-one laboratories measured EV-TF activity and antigen in the prepared samples using their own assays representing 18 functional and 9 antigenic assays.RESULTS: There was a large variability in the absolute values for the different EV-TF activity and antigen assays. Activity assays had higher specificity and sensitivity compared with antigen assays. In addition, there was a large intra-assay and interassay variability. Functional assays that used a blocking anti-TF antibody or immunocapture were the most specific and sensitive. Activity assays that used immunocapture had a lower coefficient of variation compared with assays that isolated EVs by high-speed centrifugation.CONCLUSION: Based on this multicenter study, we recommend measuring EV-TF using a functional assay in the presence of an anti-TF antibody.",
author = "Amandine Bonifay and Nigel Mackman and Yohei Hisada and Sachetto, {Ana Teresa Azevedo} and Chi Hau and Elaine Gray and John Hogwood and Anat Aharon and Lina Badimon and Lucio Barile and Justine Baudar and Lennart Beckmann and Birke Benedikter and Sara Bolis and Tarik Bouriche and Marta Brambilla and Jacopo Burrello and Marina Camera and Elena Campello and Camille Ettelaie and Doroth{\'e}e Faille and Sophie Featherby and Corentin Franco and Maite Guldenpfennig and John-Bjarne Hansen and Coralie Judicone and Yohan Kim and Kristensen, {Soren Risom} and Katrin Laakmann and Florian Langer and Nadezhda Latysheva and Fabrice Lucien and {de Menezes}, {Erika Marques} and Fran{\c c}ois Mullier and Philip Norris and Jette Nybo and Josune Orbe and Bjarne Osterud and Paramo, {Jose A} and Radu, {Claudia M} and Carmen Roncal and Nazanin Samadi and Omri Snir and Rosa Suades and Casper Wahlund and Corinne Chareyre and Evelyne Abdili and Kimberly Martinod and Johannes Thaler and Fran{\c c}oise Dignat-George and Rienk Nieuwland and Romaric Lacroix",
note = "Copyright {\textcopyright} 2024. Published by Elsevier Inc.",
year = "2024",
month = oct,
doi = "10.1016/j.jtha.2024.05.037",
language = "English",
volume = "22",
pages = "2910--2921",
journal = "J THROMB HAEMOST",
issn = "1538-7933",
publisher = "Wiley-Blackwell",
number = "10",

}

RIS

TY - JOUR

T1 - Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology

AU - Bonifay, Amandine

AU - Mackman, Nigel

AU - Hisada, Yohei

AU - Sachetto, Ana Teresa Azevedo

AU - Hau, Chi

AU - Gray, Elaine

AU - Hogwood, John

AU - Aharon, Anat

AU - Badimon, Lina

AU - Barile, Lucio

AU - Baudar, Justine

AU - Beckmann, Lennart

AU - Benedikter, Birke

AU - Bolis, Sara

AU - Bouriche, Tarik

AU - Brambilla, Marta

AU - Burrello, Jacopo

AU - Camera, Marina

AU - Campello, Elena

AU - Ettelaie, Camille

AU - Faille, Dorothée

AU - Featherby, Sophie

AU - Franco, Corentin

AU - Guldenpfennig, Maite

AU - Hansen, John-Bjarne

AU - Judicone, Coralie

AU - Kim, Yohan

AU - Kristensen, Soren Risom

AU - Laakmann, Katrin

AU - Langer, Florian

AU - Latysheva, Nadezhda

AU - Lucien, Fabrice

AU - de Menezes, Erika Marques

AU - Mullier, François

AU - Norris, Philip

AU - Nybo, Jette

AU - Orbe, Josune

AU - Osterud, Bjarne

AU - Paramo, Jose A

AU - Radu, Claudia M

AU - Roncal, Carmen

AU - Samadi, Nazanin

AU - Snir, Omri

AU - Suades, Rosa

AU - Wahlund, Casper

AU - Chareyre, Corinne

AU - Abdili, Evelyne

AU - Martinod, Kimberly

AU - Thaler, Johannes

AU - Dignat-George, Françoise

AU - Nieuwland, Rienk

AU - Lacroix, Romaric

N1 - Copyright © 2024. Published by Elsevier Inc.

PY - 2024/10

Y1 - 2024/10

N2 - BACKGROUND: Scientific and clinical interest in extracellular vesicles (EVs) is growing. EVs that expose tissue factor (TF) bind factor VII/VIIa and can trigger coagulation. Highly procoagulant TF-exposing EVs are detectable in the circulation in various diseases, such as sepsis, COVID-19, or cancer. Many in-house and commercially available assays have been developed to measure EV-TF activity and antigen, but only a few studies have compared some of these assays.OBJECTIVES: The International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Subcommittee on Vascular Biology initiated a multicenter study to compare the sensitivity, specificity, and reproducibility of these assays.METHODS: Platelet-depleted plasma samples were prepared from blood of healthy donors. The plasma samples were spiked either with EVs from human milk or EVs from TF-positive and TF-negative cell lines. Plasma was also prepared from whole human blood with or without lipopolysaccharide stimulation. Twenty-one laboratories measured EV-TF activity and antigen in the prepared samples using their own assays representing 18 functional and 9 antigenic assays.RESULTS: There was a large variability in the absolute values for the different EV-TF activity and antigen assays. Activity assays had higher specificity and sensitivity compared with antigen assays. In addition, there was a large intra-assay and interassay variability. Functional assays that used a blocking anti-TF antibody or immunocapture were the most specific and sensitive. Activity assays that used immunocapture had a lower coefficient of variation compared with assays that isolated EVs by high-speed centrifugation.CONCLUSION: Based on this multicenter study, we recommend measuring EV-TF using a functional assay in the presence of an anti-TF antibody.

AB - BACKGROUND: Scientific and clinical interest in extracellular vesicles (EVs) is growing. EVs that expose tissue factor (TF) bind factor VII/VIIa and can trigger coagulation. Highly procoagulant TF-exposing EVs are detectable in the circulation in various diseases, such as sepsis, COVID-19, or cancer. Many in-house and commercially available assays have been developed to measure EV-TF activity and antigen, but only a few studies have compared some of these assays.OBJECTIVES: The International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Subcommittee on Vascular Biology initiated a multicenter study to compare the sensitivity, specificity, and reproducibility of these assays.METHODS: Platelet-depleted plasma samples were prepared from blood of healthy donors. The plasma samples were spiked either with EVs from human milk or EVs from TF-positive and TF-negative cell lines. Plasma was also prepared from whole human blood with or without lipopolysaccharide stimulation. Twenty-one laboratories measured EV-TF activity and antigen in the prepared samples using their own assays representing 18 functional and 9 antigenic assays.RESULTS: There was a large variability in the absolute values for the different EV-TF activity and antigen assays. Activity assays had higher specificity and sensitivity compared with antigen assays. In addition, there was a large intra-assay and interassay variability. Functional assays that used a blocking anti-TF antibody or immunocapture were the most specific and sensitive. Activity assays that used immunocapture had a lower coefficient of variation compared with assays that isolated EVs by high-speed centrifugation.CONCLUSION: Based on this multicenter study, we recommend measuring EV-TF using a functional assay in the presence of an anti-TF antibody.

U2 - 10.1016/j.jtha.2024.05.037

DO - 10.1016/j.jtha.2024.05.037

M3 - SCORING: Journal article

C2 - 38925490

VL - 22

SP - 2910

EP - 2921

JO - J THROMB HAEMOST

JF - J THROMB HAEMOST

SN - 1538-7933

IS - 10

ER -