Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology
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Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology. / Bonifay, Amandine; Mackman, Nigel; Hisada, Yohei; Sachetto, Ana Teresa Azevedo; Hau, Chi; Gray, Elaine; Hogwood, John; Aharon, Anat; Badimon, Lina; Barile, Lucio; Baudar, Justine; Beckmann, Lennart; Benedikter, Birke; Bolis, Sara; Bouriche, Tarik; Brambilla, Marta; Burrello, Jacopo; Camera, Marina; Campello, Elena; Ettelaie, Camille; Faille, Dorothée; Featherby, Sophie; Franco, Corentin; Guldenpfennig, Maite; Hansen, John-Bjarne; Judicone, Coralie; Kim, Yohan; Kristensen, Soren Risom; Laakmann, Katrin; Langer, Florian; Latysheva, Nadezhda; Lucien, Fabrice; de Menezes, Erika Marques; Mullier, François; Norris, Philip; Nybo, Jette; Orbe, Josune; Osterud, Bjarne; Paramo, Jose A; Radu, Claudia M; Roncal, Carmen; Samadi, Nazanin; Snir, Omri; Suades, Rosa; Wahlund, Casper; Chareyre, Corinne; Abdili, Evelyne; Martinod, Kimberly; Thaler, Johannes; Dignat-George, Françoise; Nieuwland, Rienk; Lacroix, Romaric.
in: J THROMB HAEMOST, Jahrgang 22, Nr. 10, 10.2024, S. 2910-2921.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Comparison of assays measuring extracellular vesicle-tissue factor in plasma samples: Communication from the ISTH SSC Subcommittee on Vascular Biology
AU - Bonifay, Amandine
AU - Mackman, Nigel
AU - Hisada, Yohei
AU - Sachetto, Ana Teresa Azevedo
AU - Hau, Chi
AU - Gray, Elaine
AU - Hogwood, John
AU - Aharon, Anat
AU - Badimon, Lina
AU - Barile, Lucio
AU - Baudar, Justine
AU - Beckmann, Lennart
AU - Benedikter, Birke
AU - Bolis, Sara
AU - Bouriche, Tarik
AU - Brambilla, Marta
AU - Burrello, Jacopo
AU - Camera, Marina
AU - Campello, Elena
AU - Ettelaie, Camille
AU - Faille, Dorothée
AU - Featherby, Sophie
AU - Franco, Corentin
AU - Guldenpfennig, Maite
AU - Hansen, John-Bjarne
AU - Judicone, Coralie
AU - Kim, Yohan
AU - Kristensen, Soren Risom
AU - Laakmann, Katrin
AU - Langer, Florian
AU - Latysheva, Nadezhda
AU - Lucien, Fabrice
AU - de Menezes, Erika Marques
AU - Mullier, François
AU - Norris, Philip
AU - Nybo, Jette
AU - Orbe, Josune
AU - Osterud, Bjarne
AU - Paramo, Jose A
AU - Radu, Claudia M
AU - Roncal, Carmen
AU - Samadi, Nazanin
AU - Snir, Omri
AU - Suades, Rosa
AU - Wahlund, Casper
AU - Chareyre, Corinne
AU - Abdili, Evelyne
AU - Martinod, Kimberly
AU - Thaler, Johannes
AU - Dignat-George, Françoise
AU - Nieuwland, Rienk
AU - Lacroix, Romaric
N1 - Copyright © 2024. Published by Elsevier Inc.
PY - 2024/10
Y1 - 2024/10
N2 - BACKGROUND: Scientific and clinical interest in extracellular vesicles (EVs) is growing. EVs that expose tissue factor (TF) bind factor VII/VIIa and can trigger coagulation. Highly procoagulant TF-exposing EVs are detectable in the circulation in various diseases, such as sepsis, COVID-19, or cancer. Many in-house and commercially available assays have been developed to measure EV-TF activity and antigen, but only a few studies have compared some of these assays.OBJECTIVES: The International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Subcommittee on Vascular Biology initiated a multicenter study to compare the sensitivity, specificity, and reproducibility of these assays.METHODS: Platelet-depleted plasma samples were prepared from blood of healthy donors. The plasma samples were spiked either with EVs from human milk or EVs from TF-positive and TF-negative cell lines. Plasma was also prepared from whole human blood with or without lipopolysaccharide stimulation. Twenty-one laboratories measured EV-TF activity and antigen in the prepared samples using their own assays representing 18 functional and 9 antigenic assays.RESULTS: There was a large variability in the absolute values for the different EV-TF activity and antigen assays. Activity assays had higher specificity and sensitivity compared with antigen assays. In addition, there was a large intra-assay and interassay variability. Functional assays that used a blocking anti-TF antibody or immunocapture were the most specific and sensitive. Activity assays that used immunocapture had a lower coefficient of variation compared with assays that isolated EVs by high-speed centrifugation.CONCLUSION: Based on this multicenter study, we recommend measuring EV-TF using a functional assay in the presence of an anti-TF antibody.
AB - BACKGROUND: Scientific and clinical interest in extracellular vesicles (EVs) is growing. EVs that expose tissue factor (TF) bind factor VII/VIIa and can trigger coagulation. Highly procoagulant TF-exposing EVs are detectable in the circulation in various diseases, such as sepsis, COVID-19, or cancer. Many in-house and commercially available assays have been developed to measure EV-TF activity and antigen, but only a few studies have compared some of these assays.OBJECTIVES: The International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Subcommittee on Vascular Biology initiated a multicenter study to compare the sensitivity, specificity, and reproducibility of these assays.METHODS: Platelet-depleted plasma samples were prepared from blood of healthy donors. The plasma samples were spiked either with EVs from human milk or EVs from TF-positive and TF-negative cell lines. Plasma was also prepared from whole human blood with or without lipopolysaccharide stimulation. Twenty-one laboratories measured EV-TF activity and antigen in the prepared samples using their own assays representing 18 functional and 9 antigenic assays.RESULTS: There was a large variability in the absolute values for the different EV-TF activity and antigen assays. Activity assays had higher specificity and sensitivity compared with antigen assays. In addition, there was a large intra-assay and interassay variability. Functional assays that used a blocking anti-TF antibody or immunocapture were the most specific and sensitive. Activity assays that used immunocapture had a lower coefficient of variation compared with assays that isolated EVs by high-speed centrifugation.CONCLUSION: Based on this multicenter study, we recommend measuring EV-TF using a functional assay in the presence of an anti-TF antibody.
U2 - 10.1016/j.jtha.2024.05.037
DO - 10.1016/j.jtha.2024.05.037
M3 - SCORING: Journal article
C2 - 38925490
VL - 22
SP - 2910
EP - 2921
JO - J THROMB HAEMOST
JF - J THROMB HAEMOST
SN - 1538-7933
IS - 10
ER -